The New York Structural Biology Center (NYSBC) is a unique facility of latest-generation instrumentation in structural biology. This is the only facility in the United States for biomolecular structure determination that provides such advanced resources in all three major technologies - nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography and cryo-electron microscopy. It is owned and operated by a consortium comprised of the 10 New York academic research institutions that includes Icahn School of Medicine.
The NYSBC is located at 89 Convent Avenue, at 133rd Street on the CUNY City College campus and has following instrumentation in NMR spectroscopy, X-ray crystallography and cryo-electron microscopy:
Nuclear Magnetic Resonance Spectrometers
The NYSBC operates a collection of ultra-high-field NMR spectrometers from Bruker that include two 900 MHz systems, three 800 MHz systems, one 700 MHz system, one 600 and one 500 MHz systems; and one 750/89 solids spectrometer for 1H/13C/15N experiments with MAS. All the liquid state NMR instruments at the NYSBC are equipped with high sensitivity cryogenic probes.
The NYSBC operates synchrotron beamlines at the Brookhaven National Laboratory on Long Island. Its X4A beamline is a tunable, multi-wavelength (MAD) beamline equipped with a CCD detector, and a second beamline X4C is designed to have a higher flux density than that of X4A.
The NYSBC supports 3D structure determination of macromolecular complexes using methods of electron crystallography, single particle analysis and electron tomography. The Cryo-EM Facility operates a JEOL 300kV Field Emission Gun (FEG) electron microscope with liquid He stage in-column energy filter and a 4kx4k CCD camera, two 200kV FEG electron microscope systems (a JEOL 2100F and a FEI Tecnai TF20), and a JEOL 120kV microscope. Sample preparation accessories, film densitometers, and computing processing facilities are available to support applications in tomography, two-dimensional crystallography, and single particle reconstruction.
Ming-Ming Zhou, PhD
Aneel Aggarwal, PhD
Iban Ubarretxena-Belandia, PhD
David Cowburn, PhD
President and CEO at the NYSBC