Carlo Iomini, PhD
- ASSISTANT PROFESSOR | Ophthalmology
Research Topics:Cell Biology, Cell Motility, Cytoskeleton, Developmental Biology, Developmental Neurobiology, Epithelial Cells, Microtubules, Molecular Motors, Organogenesis, Proteomics, Vision
M.S. (Diploma di Laurea), University of Milano
Ph.D., Museum National d'Histoire Naturelle, Paris
Mount Sinai School of Medicine, New York
Washington University, St. Louis
Dolly Green Special Scholar Award
Young Investigator Award
Eye patterning, morphogenesis and diseases
The Iomini Lab is interested in understanding the role of primary cilia during tissue morphogenesis and repair. We are using the anterior segment of the eye as a novel paradigm to address this question. Our approach involves mouse genetics in combination with advance microscopy, genomic technology and biochemistry.
Primary cilia are microtubule-based cellular organelles that assemble at the cellular surface and organize sensory transduction including Shh, calcium and cell polarization signaling. Although several ultrastructural studies documented cilia morphology and presence in many vertebrate tissues and cell types it wasn’t until recent years that their protein composition started to be uncovered. However, much remains to be determined regarding their biogenesis and function during development, homeostasis and regeneration of complex tissues. Disfunctional cilia lead to a class of inherited human diseases collectively called “ciliopathies” and characterized by loss of vision, obesity, mental retardation and morphogenetic defects in many tissues. Ciliopathies such as Meckel, Bardet-Biedel and Joubert syndromes present conditions of the anterior segment of the eye including poorly understood disorders such as abnormal iridocorneal angle, sclerocornea, keratoconus, microphthalmia, microcornea, glaucoma, corneal thickening, edema, and cataract.
We have recently shown that primary cilia play an essential role in instructing cellular remodeling and tissue morphogenesis during development and repair of corneal cell layers. Our long-term goals are:
1) To elucidate the role of primary cilia in signaling pathways required for proper cell differentiation and tissue patterning during corneal development.
2) To identify new cilia-related therapeutic targets to counteract corneal cell loss, due to aging, trauma or diseases, and improve corneal repair.
3) To uncover the role of primary cilia during eye development with a particular focus on congenital anterior segment diseases and glaucoma.
Balmer S, Dussert A, Collu GM, Benitez E, Iomini C, Mlodzik M. Components of Intraflagellar Transport Complex A Function Independently of the Cilium to Regulate Canonical Wnt Signaling in Drosophila. Developmental cell 2015 Sep; 34(6).
Luo N, Conwell MD, Chen X, Kettenhofen CI, Westlake CJ, Cantor LB, Wells CD, Weinreb RN, Corson TW, Spandau DF, Joos KM, Iomini C, Obukhov AG, Sun Y. Primary cilia signaling mediates intraocular pressure sensation. Proceedings of the National Academy of Sciences of the United States of America 2014 Sep; 111(35).
Gentile RC, Iomini C. Beating Cilia and Whipping Flagella: More Than Meets the Eye. Journal of pediatric ophthalmology and strabismus; 52(3).
Aguilar A, Becker L, Tedeschi T, Heller S, Iomini C, Nachury MV. Α-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion. Molecular biology of the cell 2014 Jun; 25(12).
Blitzer AL, Panagis L, Gusella GL, Danias J, Mlodzik M, Iomini C. Primary cilia dynamics instruct tissue patterning and repair of corneal endothelium. Proceedings of the National Academy of Sciences of the United States of America 2011 Feb; 108(7).
Li X, Iomini C, Hyink D, Wilson PD. PRKX critically regulates endothelial cell proliferation, migration, and vascular-like structure formation. Developmental biology 2011 Aug; 356(2).
Iomini C, Li L, Esparza JM, Dutcher SK. Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 2009 Nov; 183(3).
Iomini C, Till JE, Dutcher SK. Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii. Methods in cell biology 2009; 93.
Iomini C, Li L, Mo W, Dutcher SK, Piperno G. Two flagellar genes, AGG2 and AGG3, mediate orientation to light in Chlamydomonas. Current biology : CB 2006 Jun; 16(11).
Iomini C, Tejada K, Mo W, Vaananen H, Piperno G. Primary cilia of human endothelial cells disassemble under laminar shear stress. The Journal of cell biology 2004 Mar; 164(6).
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G. Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. The Journal of cell biology 2001 Apr; 153(1).