- ASSISTANT PROFESSOR Pharmacology and Systems Therapeutics
MS, Basel University
Ph.D., Basel Institute for Immunology/Basel University
Postdoctoral Fellow, Princeton University
Stem Cell Biology
Reprogramming, disease modeling and therapeutics with pluripotnent stem cellsOur research interests are to investigate the molecular mechanisms of (re)programming cell fate changes and to study disease pathology and facilitate the development of novel therapeutic strategies with induced pluripotent stem cells. The revolutionary discovery that specialized, adult somatic cells when exposed to pluripotency-associated factors and/or microRNAs, become plastic and regain embryonic stem cell (ESC) characteristics was a ground-breaking advance in stem cell research. These so-called induced pluripotent stem cell (iPSCs) generate an unlimited source of cells to model human diseases in a dish, to develop diagnostics and novel therapeutic strategies. This model allows us to test the efficacy and safety of different small molecule/drug treatments by using a “virtual clinical trial in a dish” format. Target cells differentiated from patient-specific iPSCs and disease-corrected hiPSC can be used to develop personalized pharmacologic treatments to which patients show the best response. Additionally, they can be used to screen for (novel) small compounds that can ameliorate the patient’s functional defect.
Gaspar-Maia A, Qadeer ZA, Hasson D, Ratnakumar K, Adrian Leu N, Leroy G, Liu S, Costanzi C, Valle-Garcia D, Schaniel C, Lemischka I, Garcia B, Pehrson JR, Bernstein E. MacroH2A histone variants act as a barrier upon reprogramming towards pluripotency. Nature Communications 2013; 4: 1565.
Young W, D'Souza SL, Lemischka IR, Schaniel C. Patient-specific induced pluripotent stem cells as a platform for disease modeling, drug discovery and precision personalized medicine. The Journal of Stem Cell Research & Therapy 2012; S10-010.
Lee DF, Su J, Ang YS, Carvajal-Vergara X, Mulero-Navarro S, Pereira CF, Gingold J, Wang HL, Zhao R, Sevilla A, Darr H, Williamson AJ, Chang B, Niu X, Aguilo F, Flores ER, Sher YP, Hung MC, Whetton AD, Gelb BD, Moore KA, Snoeck HW, Ma'ayan A, Schaniel C, Lemischka IR. Regulation of embryonic and induced pluripotency by Aurora kinase-p53 signaling. Cell Stem Cell 2012; 11(2): 179-194.
Lee DF, Su J, Sevilla A, Gingold J, Schaniel C, Lemischka IR. Combining competition assays with genetic complementation strategies to dissect mouse embryonic stem cell self-renewal and pluripotency. Nature Protocols 2012; 7(4): 729-748.
Chang B, Lemischka IR, Schaniel C. MicroRNAs in development, stem cell differentiation and regenerative medicine. In: Regulatory RNAs . Springer;prettyprintp, 409-442.
Schaniel C, Sirabella D, Qiu J, Niu X, Lemischka IR, Moore KA. Wnt-inhibitory factor 1 dysregulation of the bone marrow niche exhausts hematopoietic stem cells. Blood 2011; 118(9): 2420-2429.
Ang YS, Tsai SY, Lee DF, Monk J, Su J, Ratnakumar K, Ding J, Ge Y, Darr H, Chang B, Wang J, Rendl M, Bernstein E, Schaniel C, Lemischka IR. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011; 145(2): 183-197.
Green MD, Chen A, Nostro MC, d'Souza SL, Schaniel C, Lemischka IR, Gouon-Evans V, Keller G, Snoeck HW. Generation of anterior foregut endoderm from human embryonic and induced pluripotent stem cells. Nature Biotechnology 2011; 29(3): 267-272.
Xu H, Schaniel C, Lemischka IR, Ma'ayan A. Toward a complete in silico, multi-layered embryonic stem cell regulatory network [review]. Wiley Interdisciplinary Reviews. Systems Biology and Medicine 2010; 2(6): 708-733.
Schaniel C, Lee DF, Lemischka IR, Gonsalves FC, DasGupta R. Exploration of self-renewal and pluripotency in ES cells using RNAi. Methods in Enzymology 2010 477 477:351-365.
Carvajal-Vergara X, Sevilla A, D'Souza SL, Ang YS, Schaniel C, Lee D, Yang L, Kaplan AD, Adler ED, Rozov R, Ge Y, Cohen N, Edelmann LJ, Chang B, Waghray A, Su J, Pardo S, Lichtenbelt KD, Tartaglia M, Gelb BD, Lemischka IR. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome. Nature 2010; 465(7299): 808-812.
Schaniel C, Moore KA. Genetic models to study quiescent stem cells and their niches. Ann N Y Acad Sci 2009; 1176: 26-35.
Schaniel C, Ang YS, Ratnakumar K, Cormier C, James T, Bernstein E, Lemischka IR, Paddison PJ. Smarcc1/Baf155 couples self-renewal gene repression with changes in chromatin structure in mouse embryonic stem cells. Stem Cells 2009; 27(12): 2979-2991.
Schaniel C, Li F, Schafer XL, Moore T, Lemischka IR, Paddison PJ. Delivery of short hairpin RNAs--triggers of gene silencing--into mouse embryonic stem cells. Nature Methods 2006; 3(5): 397-400.
Rolink AG, Schaniel C, Melchers F. Stability and plasticity of wild-type and Pax5-deficient precursor B cells. Immunological Reviews 2002; 187: 87-95.
Ivanova NB, Dimos JT, Schaniel C, Hackney JA, Moore KA, Lemischka IR. A stem cell molecular signature. Science 2002; 298(5593): 601-604.
Bruno L, Schaniel C, Rolink A. Plasticity of Pax-5(-/-) pre-B I cells. Cells, Tissues, Organs 2002; 171(1): 38-43.
Rolink AG, Schaniel C, Bruno L, Melchers F. In vitro and in vivo plasticity of Pax5-deficient pre-B I cells. Immunology Letters 2002; 82(1-2): 35-40.
Schaniel C, Gottar M, Roosnek E, Melchers F, Rolink AG. Extensive in vivo self-renewal, long-term reconstitution capacity, and hematopoietic multipotency of Pax5-deficient precursor B-cell clones. Blood 2002; 99(8): 2760-2766.
Schaniel C, Bruno L, Melchers F, Rolink AG. Multiple hematopoietic cell lineages develop in vivo from transplanted Pax5-deficient pre-B I-cell clones. Blood 2002; 99(2): 472-478.
Ghia P, Transidico P, Veiga JP, Schaniel C, Sallusto F, Matsushima K, Sallan SE, Rolink AG, Mantovani A, Nadler LM, Cardoso AA. Chemoattractants MDC and TARC are secreted by malignant B-cell precursors following CD40 ligation and support the migration of leukemia-specific T cells. Blood 2001; 98(3): 533-540.
Schaniel C, Rolink AG, Melchers F. Attractions and migrations of lymphoid cells in the organization of humoral immune responses. Advances in Immunology 2001; 78: 111-168.
Rolink AG, Schaniel C, Andersson J, Melchers F. Selection events operating at various stages in B cell development. Current Opinion in Immunology 2001; 13(2): 202-207.
Schaniel C, Melchers F, Rolink AG. The cluster of ABCD chemokines which organizes T cell-dependent B cell responses. Current Topics in Microbiology and Immunology 2000; 251: 181-189.
Rolink AG, Schaniel C, Busslinger M, Nutt SL, Melchers F. Fidelity and infidelity in commitment to B-lymphocyte lineage development. Immunological Reviews 2000; 175: 104-111.
Melchers F, Rolink AG, Schaniel C. The role of chemokines in regulating cell migration during humoral immune responses. Cell 1999; 99(4): 351-354.
Yamagami T, ten Boekel E, Schaniel C, Andersson J, Rolink A, Melchers F. Four of five RAG-expressing JCkappa-/- small pre-BII cells have no L chain gene rearrangements: detection by high-efficiency single cell PCR. Immunity 1999; 11(3): 309-316.
Schaniel C, Sallusto F, Ruedl C, Sideras P, Melchers F, Rolink AG. Three chemokines with potential functions in T lymphocyte-independent and -dependent B lymphocyte stimulation. European Journal of Immunology 1999; 29(9): 2934-2947.
Ghia P, Schaniel C, Rolink AG, Nadler LM, Cardoso AA. Human macrophage-derived chemokine (MDC) is strongly expressed following activation of both normal and malignant precursor and mature B cells. Current Topics in Microbiology and Immunology 1999; 246: 103-110.
Schaniel C, Sallusto F, Sideras P, Melchers F, Rolink AG. A novel CC chemokine ABCD-1, produced by dendritic cells and activated B cells, exclusively attracts activated T lymphocytes. Current Topics in Microbiology and Immunology 1999; 246: 95-101.
Sallusto F, Schaerli P, Loetscher P, Schaniel C, Lenig D, Mackay CR, Qin S, Lanzavecchia A. Rapid and coordinated switch in chemokine receptor expression during dendritic cell maturation. European Journal of Immunology 1998; 28(9): 2760-2769.
Schaniel C, Pardali E, Sallusto F, Speletas M, Ruedl C, Shimizu T, Seidl T, Andersson J, Melchers F, Rolink AG, Sideras P. Activated murine B lymphocytes and dendritic cells produce a novel CC chemokine which acts selectively on activated T cells. The Journal of Experimental Medicine 1998; 188(3): 451-463.
Physicians and scientists on the faculty of the Icahn School of Medicine at Mount Sinai often interact with pharmaceutical, device and biotechnology companies to improve patient care, develop new therapies and achieve scientific breakthroughs. In order to promote an ethical and transparent environment for conducting research, providing clinical care and teaching, Mount Sinai requires that salaried faculty inform the School of their relationships with such companies.
Dr. Schaniel did not report having any of the following types of financial relationships with industry during 2012 and/or 2013: consulting, scientific advisory board, industry-sponsored lectures, service on Board of Directors, participation on industry-sponsored committees, equity ownership valued at greater than 5% of a publicly traded company or any value in a privately held company. Please note that this information may differ from information posted on corporate sites due to timing or classification differences.
Mount Sinai's faculty policies relating to faculty collaboration with industry are posted on our website at http://icahn.mssm.edu/about-us/services-and-resources/faculty-resources/handbooks-and-policies/faculty-handbook. Patients may wish to ask their physician about the activities they perform for companies.
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