Genomic Technology Applications

At the Icahn Institute and Department of Genetics and Genomic Sciences, we use next-generation sequencing platforms to create a genome-wide view of biology. Below are services currently available from our genomics facility:

Genetic Event/Application

Method

Available Technology

Single molecule sequencing

Amplification free library preparation

PacBio RS

5-Methylcytosine

Methylation arrays

Bisulfite sequencing

Illumina BeadArray

Illumina HiSeq 2500

PacBio RS

Discovery of novel epigenetic modifications

Direct observation of polymerase kinetics

PacBio RS

Whole-genome sequencing for organisms with no genome assembly (bacteria, viruses, non-model organisms)

Amplification-free 2kb library preparation Illumina gDNA library preparation

PacBio RS Illumina HiSeq 2500

Somatic and germ line mutations, deletions, genomic rearrangements and CNVs

Genotyping by microarray Whole-Exome sequencing Amplicon sequencing

Illumina BeadArray

Illumina HiSeq 2500

PacBio RS

Chromatin structure and in-vivo protein/DNA interactions

Chromatin Immuno-precipitation and library preparation ("ChIP-Seq")

Illumina HiSeq 2500

Illumina MiSeq

Plasmid/Amplicon Sequencing

Sanger dye-terminator sequencing

AB 3730xl Capillary Sequener

Gene expression

cDNA library construction ("RNA-Seq")

cRNA direct Hybridization

Illumina HiSeq 2500

PacBio RS

Alternative splicing

cDNA library construction followed by junction analysis

Illumina HiSeq 2500

PacBio RS

Small RNA profiling and discovery

RNA adapter ligation and PCR

Illumina MiSeq

Illumina HiSeq2500