Production of Human Monoclonal Antibodies

To circumvent the anti-species response, technology for the direct production of human mAbs are currently being developed. Depending upon the immune status of the donor (patient or subject), antibody secreting B cells can be isolated through a number of methods that are currently available in our facility.

Human monoclonal antibodies are being produced by multiple methods including in vitro immortalization of memory B cell and V gene cloning from antigen specific B cells single cell sorted on a FACS sorter. The Center for Therapeutic Antibody Development facility utilizes molecular technology to generate V gene clones from the isolated monoclonal antibody producing B cells for the purposes of generating recombinant versions of the antibodies for production, characterization, genetic manipulation, and DNA storage. Sophisticated analysis of antibody specificity and binding is being performed (surface plasmon resonance, etc) to maintain binding affinity and specificity of cloned molecules.

Humanized Antibody