Technology Platforms

DNA Sequencing and nucleic acid characterization instruments

Illumina HiSeq 2000 – second generation sequencer from Illumina; capacity of 120-150M reads of 100 nt in length. Libraries may be sequenced from one or both ends
Illumina MiSeq – “personal” second generation sequencer based on HiSeq chemistry; capacity of 12-15M reads in 24 hours
Pacific Biosciences RS – third generation single molecule sequencer with read lengths up to 6kb
Applied Biosystems 3730xl – 96 capillary dye-terminator sequencer
Applied Biosystems 7900HT – real-time thermocycler for quantitative PCR and end-point allele discrimination for use by GGS department members

Illumina HiScan – most adavanced bead-array reader on the market for high-density SNP detection, mRNA-profiling and methylation microarrays
Illumina BeadXPress - medium density, custom SNP detection platform

Covaris E210 Focused Acoustic Disrupter – sonicator for automated high yield preparation of multiple genomic/mRNA libraries
Two TECAN Liquid Handling robot – Automated Bead Array hybridization and washing
Three Agilent BioAnalyzer – microfluidics-based platform for sizing, quantification and quality control of DNA and RNA; crucial in preparation of libraries for Illumina sequencing
Agilent Bravo - sample preparation of Illumina sequencing libraries using SPRI magnetic bead technology
Beckman BioMek FX liquid handling robot – automated Sanger sequencing reaction cleanup and real-time PCR plate set-up