Techniques

Sendai viral reprogramming techniques are used to reprogram patient fibroblasts to induced pluripotent stem cells. We are continuously testing and adapting state-of-the-art neural differentiation protocols to improve the yield and purity of glutamatergic, GABAergic, and dopaminergic neurons generated from our hiPSCs. Molecular profiles of control and SZ neural progenitor cells and neurons are compared by RNAseq and microarrays. Cellular phenotypes of NPCs are identified by assays for cellular replication, death, oxidative stress, and migration. SZ neurons are phenotyping by assays for dendritic arborization, synaptic density, rabies virus transneuronal tracing, calcium imaging, and electrophysiology.

Contact Us

Kristen Brennand, PhD
Office: 212-659-8259
Laboratory: 212-803-6740
Fax: 212-803-6740
E-mail: kristen.brennand@mssm.edu

Icahn Medical Institute
1425 Madison Avenue
13th Floor, Room 13-23B
New York, NY 10029