Selected Publications

Janto B, Ahmed A, Ito M, Liu J, Hicks DB, Pagni S, Fackelmayer OJ, Smith TA, Earl J, Elbourne LD, Hassan K, Paulsen IT, Kolstø AB, Tourasse NJ, Ehrlich GD, Boissy R, Ivey DM, Li G, Xue Y, Ma Y, Hu FZ, Krulwich TA. Environ Microbiol. 2011 Dec;13(12):3289-309. 

Liu J, Fackelmayer OJ, Hicks DB, Preiss L, Meier T, Sobie EA, Krulwich TA. Mutations in a helix-1 motif of the ATP synthase c-Subunit of Bacillus pseudofirmus OF4 cause functional deficits and changes in the c-ring stability and mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Biochemistry. 2011 Jun 21;50(24):5497-506.

Krulwich TA, Sachs G, Padan E. Molecular aspects of bacterial pH sensing and homeostasis. Nat Rev Microbiol. 2011 May;9(5):330-43. 

Hoffmann J, Sokolova L, Preiss L, Hicks DB, Krulwich TA, Morgner N, Wittig I, Schägger H, Meier T, Brutschy B. ATP synthases: cellular nanomotors characterized by LILBID mass spectrometry. Phys Chem Chem Phys. 2010 Nov 7;12(41):13375-82.

Fujisawa M, Fackelmayer O, Liu J, Krulwich TA, Hicks DB. The ATP synthase a-subunit of extreme alkaliphiles is a distinct variant. Mutations in the critical alkaliphile-specific residue Lys-180 and other residues that support alkaliphile OXPHOS. J Biol Chem. 2010 Oct 15;285(42):32105-15.

Morino M, Natsui S, Ono T, Swartz TH, Krulwich TA, Ito M. Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4 that affect Na /H antiport activity, sodium exclusion, individual Mrp protein levels or Mrp complex formation. J Biol Chem. 2010 Oct 1;285(40):30942-50.

Preiss L, Yildiz O, Hicks DB, Krulwich TA, Meier T. A new type of proton coordination in an F(1)F(o)-ATP synthase rotor ring. PLoS Biol. 2010 Aug 3;8(8):e1000443.

Hicks DB, Liu J, Fujisawa M, Krulwich TA. F(1)F(0)-ATP synthases of alkaliphilic bacteria: Lessons from their adaptations. Biochim Biophys Acta. 2010 Aug;1797(8):1362-1377.

Fujinami S, Terahara N, Krulwich TA, Ito M.  Motility and chemotaxis in alkaliphilic Bacillus species. Future Microbiol. 2009 Nov;4:1137-49.

Krulwich TA, Hicks DB, Ito M. Cation/proton antiporter complements of bacteria: why so large and diverse? Mol Microbiol. 2009 Oct;74(2):257-60.

Slonczewski JL, Fujisawa M, Dopson M, Krulwich TA. Cytoplasmic pH measurement and homeostasis in bacteria and archaea. Adv Microb Physiol. 2009;55:1-79, 317.

Liu J, Fujisawa M, Hicks DB, Krulwich TA.  Characterization of the Functionally Critical AXAXAXA and PXXEXXP Motifs of the ATP Synthase c-Subunit from an Alkaliphilic Bacillus. J Biol Chem. 2009 Mar 27;284(13):8714-25.

Terahara N, Krulwich TA, Ito M. Mutations alter the sodium versus proton use of a Bacillus clausii flagellar motor and confer dual ion use on Bacillus subtilis motors. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14359-64.

Morino M, Natsui S, Swartz TH, Krulwich TA, Ito M. Single gene deletions of mrpA to mrpG and mrpE point mutations affect activity of the Mrp Na /H antiporter of alkaliphilic Bacillus and formation of hetero-oligomeric Mrp complexes. J Bacteriol. 2008 Jun;190(12):4162-72.

Liu J, Krulwich TA, Hicks DB. Purification of two putative type II NADH dehydrogenases with different substrate specificities from alkaliphilic Bacillus pseudofirmus OF4. Biochim Biophys Acta. 2008 May;1777(5):453-61.

Fujinami S, Sato T, Trimmer JS, Spiller BW, Clapham DE, Krulwich TA, Kawagishi I, Ito M. The voltage-gated Na channel NaVBP co-localizes with methyl-accepting chemotaxis protein at cell poles of alkaliphilic Bacillus pseudofirmus OF4. Microbiology. 2007 Dec;153(Pt 12):4027-38.

Fujisawa M, Ito M, Krulwich TA. Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity. Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13289-94.

Wei Y, Liu J, Ma Y, Krulwich TA. Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant. Microbiology. 2007 Jul;153(Pt 7):2168-79.

Swartz TH, Ito M, Ohira T, Natsui S, Hicks DB, Krulwich TA. Catalytic properties of Staphylococcus aureus and Bacillus members of the secondary cation/proton antiporter-3 (Mrp) family are revealed by an optimized assay in an Escherichia coli host. J Bacteriol. 2007 Apr;189(8):3081-90.

Liu X, Gong X, Hicks DB, Krulwich TA, Yu L, Yu CA. Interaction between cytochrome caa3 and F1F0-ATP synthase of alkaliphilic Bacillus pseudofirmus OF4 is demonstrated by saturation transfer electron paramagnetic resonance and differential scanning calorimetry assays. Biochemistry. 2007 Jan 9;46(1):306-13.

Terahara N, Fujisawa M, Powers B, Henkin TM, Krulwich TA, Ito M. An intergenic stem-loop mutation in the Bacillus subtilis ccpA-motPS operon increases motPS transcription and the MotPS contribution to motility. J Bacteriol. 2006 Apr;188(7):2701-5.

Wei Y, Deikus G, Powers B, Shelden V, Krulwich TA, Bechhofer DH. Adaptive gene expression in Bacillus subtilis strains deleted for tetL. J Bacteriol. 2006 Oct;188(20):7090-100.

De Jesus M, Jin J, Guffanti AA, Krulwich TA. Importance of the GP Dipeptide of the Antiporter Motif and Other Membrane-Embedded Proline and Glycine Residues in Tetracycline Efflux Protein Tet(L). Biochemistry. 2005 Sep 27;44(38):12896-904.

Ito M, Terahara N, Fujinami S, Krulwich TA. Properties of motility in Bacillus subtilis powered by the H -coupled MotAB flagellar stator, Na -coupled MotPS or hybrid stators MotAS or MotPB. J Mol Biol. 2005 Sep 16;352(2):396-408.

Swartz TH, Ikewada S, Ishikawa O, Ito M, Krulwich TA. The Mrp system: a giant among monovalent cation/proton antiporters? Extremophiles. 2005 Oct;9(5):345-54.

Krulwich TA, Lewinson O, Padan E, Bibi E. Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study. Nat Rev Microbiol. 2005 Jul;3(7):566-72.

Rivera-Torres IO, Krueger-Koplin RD, Hicks DB, Cahill SM, Krulwich TA, Girvin ME. pKa of the essential Glu54 and backbone conformation for subunit c from the H -coupled F1F0 ATP synthase from an alkaliphilic Bacillus. FEBS Lett. 2004 Sep 24;575(1-3):131-5.

Ito M, Hicks DB, Henkin TM, Guffanti AA, Powers BD, Zvi L, Uematsu K, Krulwich TA. MotPS is the stator-force generator for motility of alkaliphilic Bacillus, and its homologue is a second functional Mot in Bacillus subtilis. Mol Microbiol. 2004 Aug;53(4):1035-49.

Ito M, Xu H, Guffanti AA, Wei Y, Zvi L, Clapham DE, Krulwich TA. The voltage-gated Na channel NaVBP has a role in motility, chemotaxis, and pH homeostasis of an alkaliphilic Bacillus. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10566-71.

Wang Z, Hicks DB, Guffanti AA, Baldwin K, Krulwich TA. Replacement of amino acid sequence features of a- and c-subunits of ATP synthases of Alkaliphilic Bacillus with the Bacillus consensus sequence results in defective oxidative phosphorylation and non-fermentative growth at pH 10.5. J Biol Chem. 2004 Jun 18;279(25):26546-54.

Krueger-Koplin RD, Sorgen PL, Krueger-Koplin ST, Rivera-Torres IO, Cahill SM, Hicks DB, Grinius L, Krulwich TA, Girvin ME. An evaluation of detergents for NMR structural studies of membrane proteins. J Biomol NMR. 2004 Jan;28(1):43-57.

Safferling M, Griffith H, Jin J, Sharp J, De Jesus M, Ng C, Krulwich TA, Wang DN. TetL tetracycline efflux protein from Bacillus subtilis is a dimer in the membrane and in detergent solution. Biochemistry. 2003 Dec 2;42(47):13969-76.

Wei Y, Southworth TW, Kloster H, Ito M, Guffanti AA, Moir A, Krulwich TA. Mutational loss of a K and NH4 transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus OF4. J Bacteriol. 2003 Sep;185(17):5133-47.

Hicks DB, Wang Z, Wei Y, Kent R, Guffanti AA, Banciu H, Bechhofer DH, Krulwich TA. A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2 uptake. Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10213-8.

Jin J, Guffanti AA, Bechhofer DH, Krulwich TA. Tet(L) and Tet(K) tetracycline-divalent metal/H antiporters: characterization of multiple catalytic modes and a mutagenesis approach to differences in their efflux substrate and coupling ion preferences. J Bacteriol. 2002 Sep;184(17):4722-32.

Jin J, Krulwich TA. Site-directed mutagenesis studies of selected motif and charged residues and of cysteines of the multifunctional tetracycline efflux protein Tet(L). J Bacteriol. 2002 Mar;184(6):1796-800.

Guffanti AA, Wei Y, Rood SV, Krulwich TA. An antiport mechanism for a member of the cation diffusion facilitator family: divalent cations efflux in exchange for K and H . Mol Microbiol. 2002 Jul;45(1):145-53.

Southworth TW, Guffanti AA, Moir A, Krulwich TA. GerN, an endospore germination protein of Bacillus cereus , is an Na( )/H( )-K( ) antiporter. J Bacteriol. 2001 Oct;183(20):5896-903.

Ito M, Guffanti AA, Krulwich TA. Mrp-dependent Na( )/H( ) antiporters of Bacillus exhibit characteristics that are unanticipated for completely secondary active transporters. FEBS Lett. 2001 May 11;496(2-3):117-20.

Krulwich TA, Jin J, Guffanti AA, Bechhofer H. Functions of tetracycline efflux proteins that do not involve tetracycline. J Mol Microbiol Biotechnol. 2001 Apr;3(2):237-46.

Jin J, Guffanti AA, Beck C, Krulwich TA. Twelve-transmembrane-segment (TMS) version (DTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity. J Bacteriol. 2001 Apr;183(8):2667-71.

Krulwich TA, Ito M, Guffanti AA. The Na( )-dependence of alkaliphily in Bacillus. Biochim Biophys Acta. 2001 May 1;1505(1):158-68.

Gilmour R, Messner P, Guffanti AA, Kent R, Scheberl A, Kendrick N, Krulwich TA. Two dimensional gel electrophoresis analyses of pH-dependent protein expression in facultatively alkaliphilic Bacillus pseudofirmus OF4 lead to characterization of an S-layer protein with a role in alkaliphily. J Bacteriol. 2000 Nov;182(21):5969-81.


Contact Us

Terry A. Krulwich, PhD
Send e-mail
Tel: 212-241-7280
Fax: 212-996-7214

Annenberg Building Room 19-24
Pharmacology and Systems Therapeutics
1468 Madison Avenue
New York NY 10029