Recognition and Publications

Recognition

Ground-breaking Paper of 2006: A bio-artificial sinus node constructed via in vivo gene transfer of an engineered pacemaker (HCN) channel reduces the dependence on electronic pacemaker in a sick sinus syndrome model. Circulation. 114(10):1000-11. Circulation, American Heart Association.

Best Basic Science Paper of 2005: Functional integration of electrically-active cardiac derivatives from genetically-engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: Insights into the development of cell-based pacemakers. Circulation. 111(1):11-20. Circulation, American Heart Association (2005).

First Prize to Ronald Li, PhD, Basic Research Faculty Award, Department of Medicine, The Johns Hopkins University (2004).

First Prize to Ronald Li, Young Investigator Award, Heart Rhythm Society (formerly known as North American Society of Pacing and Electrophysiology) (2002).

First Prize to Ronald Li, Basic Research Junior Faculty Award, Department of Medicine, The Johns Hopkins University (2002).

Research Career Development Award to Ronald Li, Cardiac Arrhythmias Research & Education Foundation, (2001).

Top Prize for Basic Research to Ronald Li, Young Investigator (Helen B. Taussig) Award, The Johns Hopkins University School of Medicine (2001).

First Prize to Ronald Li, Basic Research Fellow Award, Department of Medicine, The Johns Hopkins University (2001).

Heart and Stroke Foundation of Canada Post-doctoral Fellowship Award (1999-2001).

Selected Publications

1. Xiaolin Wang, Shuxun Chena, Marco Kong, Zuankai Wang, Kevin D. Costa, Ronald A. Li, and Dong Sun. 2011. Enhanced cell sorting and manipulation with combined optical tweezer and microfluidic chip technologies. Lab on a Chip. In press.

2. Zhang J, Calafiore M, Zeng Q, Zhang X, Huang Y, Li RA, Deng W, Zhao M. 2011. Electrically Guiding Migration of Human Induced Pluripotent Stem Cells. Stem Cell Rev. Mar 5. [Epub ahead of print]

3. Luna JI, Ciriza J, Garcia-Ojeda ME, Kong M, Herren A, Lieu DK, Li RA, Fowlkes CC, Khine M, McCloskey KE. Multiscale biomimetic topography for the alignment of neonatal and embryonic stem cell-derived heart cells. Tissue Eng Part C Methods. May;17(5):579-88.

4. Chan YC, Tse HF, Siu CW, Wang K, Li RA. 2010. Automaticity and conduction properties of bio-artificial pacemakers assessed in an in vitro monolayer model of neonatal rat ventricular myocytes. Europace. 12(8):1178-87.

5. Wilson KD, Hu S, Venkatasubrahmanyam S, Fu JD, Sun N, Abilez OJ, Baugh JJ, Jia F, Ghosh Z, Li RA, Butte AJ, Wu JC. 2010 Dynamic MicroRNA Expression Programs during Cardiac Differentiation of Human Embryonic Stem Cells: Role for miR-499. Circ Cardiovasc Genet. 3(5):426-35.

6. Liao SY, Liu Y, Siu CW, Zhang Y, Lai WH, Au KW, Lee YK, Chan YC, Yip PM, Wu EX, Wu Y, Lau CP, Li RA, Tse HF. 2010 Pro-arrhythmic Risk of Embryonic Stem Cell-Derived Cardiomyocytes Transplantation in Infarcted Myocardium. Heart Rhythm. 7(12):1852-9.

7. Hung-fat Tse, Chung-wah Siu, Song-Yan Liao, Wing-Sza Chan, Ed X Wu, Yin Wu, John Nicholls, Ronald A. Li, Michael E Benser, Stuart P Rosenberg, Euljoon Park, Chu-Pak Lau. 2010. Attenuation of left ventricular adverse remodeling with epicardial patching after myocardial infarction. Attenuation of Left Ventricular Adverse Remodeling with Epicardial Patching after Myocardial Infarction. Journal of Cardiac Failure. 16(7):590-8.

8. Kwong-Man, Lee Yee-Ki, Chan Yau-Chi, Lai Wai-hong, Fung Man-Lung, Ronald A. Li Siu Chung-Wah, Tse Hung-Fat. 2010. Exogenous Expression of HIF-1alpha Promotes Cardiac Differentiation of Embryonic Stem Cells. J Mol Cell Cardio. 48(6):1129-37

9. Fu Jidong, Peng Jiang, Ha Tran, Jing Liu, Ronald A. Li (2009). Na+/Ca2+ exchanger in hESC-derived ventricular cardiomyocytes is a functional determinant of excitation-contraction coupling during cardiac development. Stem Cell & Development. 19(6):773-82.

10. Peng Jiang, Stephanie Rushing, Jidong Fu, Wenbin Deng, Ronald A. Li. 2009. Electrophysiological Properties of Human Induced Pluripotent Stem Cells. Am J Physiol. 459(1):25-38.

11. Chung-Wah Siu, Ka-wing Au, Chu-Pak Lau, Hung-Fat Tse, Ronald A. Li (2009). State-dependent dynamic rearrangement of the HCN-encoded pacemaker channel pore. J Mem Biol. 230(1):35-47.

12. Yau-chi Chan, George Wang, Chu-Pak Lau, Hung-Fat Tse, Ronald A. Li. (2009) Probing the bradycardic drug receptor of HCN-encoded pacemaker channels. Pflugers Archive. 12(8):1178-87.

13. Jing Liu, Jidong Fu, Ronald A. Li. 2009. Functional Consequences of Overexpressing Calsequestrin in the Calcium-Handling of Human Embryonic Stem Cell-Derived Cardiomyocytes: A Strategy for Driven Maturation. Am J Physiology (Cell). 297(1):C152-9.

14. Deborah K. Lieu, Jing Liu, Chung Wah Siu, Gregory P. McNerney, Amir Abu-Khalil, Thomas Huser, Ronald A. Li (2009). The Lack of T-tubules in Human Embryonic Stem Cell-Derived Cardiomyocytes Leads to Immature Ca2+ Wave propagation. Stem Cell & Development. 18(10):1493-50.

15. Hung-Fat Tse, David Chung-Wah Siu, Virginia Yee-man Lau, Chu-Pak Lau, Ronald A. Li (2009). Synergistic interactions between the inward rectifier (IK1) and pacemaker (If) currents promote bioengineered cardiac automaticity. J Cardiac Electrophysiology. 81(4):1324-31.

16. James W. Chan, Deborah K. Lieu, Thomas Huser, Ronald A. Li (2009) Label-free spectroscopic separation of human embryonic stem cells and their cardiac derivatives. Analytical Chemistry. 81(4):1324-31.

17. Ka-Wing Au, Song-Yan Liao, Yee-Ki Lee, Wing-Hon Lai, Man-Kwong Ng, Yau-Chi Chan, Mei-Chu Yip, Chung-Yee Ho, EdX Wu, Ronald A. Li, Chung-Wah Siu, Hung-Fat Tse. (2009) Effects of Iron Oxide Nanoparticles on Cardiac Differentiation of Embryonic Stem Cells. Biochem Biophy Res Co. 379(4):898-903.

18. Deborah K. Lieu, Yau-chi Chan, Chu-Pak Lau, Hung-Fat Tse, David Chung-Wah Siu, Ronald A. Li (2008). Over-expression of HCN-encoded pacemaker current silences bio-artificial pacemakers. Heart Rhythms. 5(9):1310-7. See Commentary 5(9):1318.

19. Feng Cao, Roger A. Wagner, Kitchener D. Wilson, Xiaoyan Xie, Micha Drukker, Thomas Quertermous, Ronald A. Li, Sanjiv S. Gambhir, Irving L. Weissman, Robert C. Robbins, Joseph C. Wu (2008). Transcriptional and Functional Profiling of Human Embryonic Stem Cell-Derived Cardiomyocytes. Plos ONE. 3(10): e3474.

20. Jennifer C. Moore, Suk-ying Tsang, Stephanie Rushing, David Chung-Wah Siu, Rika van Huizen, Hung-Fat Tse, Camie W. Chan, Ronald A. Li (2008). Functional consequences of overexpression the gap junction protein Cx43 in the cardiogenic potential of human embryonic stem cells. Biochem Biophy Res Co. 377(1):46-51.

21. Kirill V. Tarasov, Yelena S. Tarasova, Wai Leong Tam, Steven T. Elliott, Daniel R. Riordon, Gianluca Testa, Gabriela Kania, Maria Volkova, Jinliang Li, David G. Crider, Faye Tsang, Sergey V. Anisimov, Ronald A. Li, Colin L. Stewart, Robert P. Wersto, Jennifer E. Van Eyk, Anna M. Wobus and Kenneth R. Boheler (2008). MYBL2 (B-MYB) is an Essential Transcription Factor Required for the Regulation of pou5f1 (oct4) and the Maintenance of Embryonic Stem Cells in the Undifferentiated State. PloS ONE. 3(6):e2478.

22. Jennifer C. Moore, David Chung-Wah Siu, Jidong Fu, Ha Tran, Yau-Chi Chan, Chu-Pak Lau, Camie Chan, Hung-Fat Tse, Ronald A. Li (2008). Distinct preferences of two human embryonic stem cell (hESC) lines to differentiate into chamber-specific cardiomyocytes and the underlying basis, as probed by electrophysiological and proteomic analyses. Biochem Biophy Res Co. 372(4):553-8.

23. Jidong Fu, Camie W. Chan, Ronald A. Li (2008). An inducible transgene expression system for conditional phenotypic modification of human embryonic stem cells. Stem Cells Dev. 17(2):315-24.

24. Karen Au, David Chung-Wah Siu, Chu-Pak Lau, Hung-Fat Tse, Ronald A. Li (2008). Structural and functional determinants in the S5-P linker of HCN-encoded pacemaker channels. Am J Physiology (Cell). 294(1):C136-44.

25. Jing Liu, Jidong Fu, Ronald A. Li. 2007. Functional Sarcoplasmic Reticulum for Calcium-Handling of Human Embryonic Stem Cell-Derived Cardiomyocytes: Insights for Driven Maturation. Stem Cells. 25(12):3038-44.

26. David Chung-Wah Siu, Jennifer Moore, Ronald A. Li (2007). Human Embryonic Stem Cell-Derived Cardiomyocytes as a Potential Solution to Heart Diseases. Cardiovascular & Hematological Disorders - Drug Targets. 7(2):145-52.

27. David Chung-Wah Siu, Deborah K. Lieu, Ronald A. Li (2007). HCN-encoded Pacemaker Channels: From Physiology, Biophysics to Bioengineering. J. Mem. Biol. 214(3):115-22.

28. Tian Xue, David Chung-Wah Siu, Deborah K. Lieu, Chu-Pak Lau, Hung-Fat Tse, Ronald A. Li (2007). Mechanistic role of If revealed by induction of ventricular automaticity by somatic gene transfer of gating-engineered pacemaker (HCN) channels. Circulation. 115(14):1839-50.

29. Jennifer C. Moore, Suk-ying Tsang, Rika van Huizen, Camie W. Chan, Ronald A. Li (2007). Ectopic expression of systemic RNA interference defective protein (SID-1) in embryonic stem cells. Biochem Biophy Res Co. 357(2):480-6.

30. Wasim El-kholy, Tian Xue, Jingyu Diao, Catherine B. Chan, Xiaolin Wang, Patrick E. MacDonald, Ronald A. Li, Robert G. Tsushima, Michael B. Wheeler (2007). Hyperpolarization-activated currents in pancreatic β cells modulate insulin secretion . Mol. Endocrinology. 21(3):753-64.

31. Hung-Fat Tse, Chu-Pak Lau, Chung-Wah Siu, Kai Wang, Qing-Yong Zhang, Tian Xue, Ronald A. Li (2006). A bio-artificial sinus node constructed via in vivo gene transfer of an engineered pacemaker (HCN) channel reduces the dependence on electronic pacemaker in a sick sinus syndrome model. Circulation. 114(10):1000-11. See Editorial Comments "Change in Pacing Paradigm" in the same issue 114(10):986-8. Groundbreaking Study of 2006.

32. Ronald A. Li, Jennifer Moore, Yelena Tarasova, Kenneth R. Boheler (2006). Human Embryonic Stem Cell-Derived Cardiomyocytes: Therapeutic Potentials and Limitations. Journal of Stem Cells. 1(2):109.

33. Wei Xiong, Ronald A. Li and Gordon F. Tomaselli. (2006). A conserved ring of charge in mammalian Na+ channels: a molecular regulator of the outer pore conformation during slow inactivation. J. Physiol. 2006 576:739-54.

34. George Wang, Tian Xue, Suk-ying Tsang, Johnny Wong, Linzhao Cheng, Janet Zhang, Guirong Li, Chu-Pak Lau, Hung-Fat Tse, Ronald A. Li. (2005) Electrophysiological properties of pluripotent human and mouse embryonic stem cells. Stem Cells. 23(10):1526-34.

35. Jennifer C. Moore, Linda W. van Laake, Stefan R. Braahm, Tian Xue, Suk-Ying Tsang, Dorien Ward, Robert Passier, Leon L. Tertoolen, Ronald A. Li, and Christine L. Mummery. 2005. Human Embryonic Stem Cells: Genetic Manipulation on the Way to Cardiac Cell Therapies. Reproductive Toxicology. 20(3):377-91.

36. Ezana Azene, Eduardo Marban, Gordon F. Tomaselli, Ronald A. Li (2005). Non-equilibrium behavior of HCN channels: Insights into the role of HCN channels in native and engineered pacemakers. Cardiovas. Res. 67(2): 263-73.

37. Tian, Xue, Ronald A. Li (2005). Circulation. 112(6):e82-83.

38. Roselle Abraham, Charles Henrikson, Leslie Tung, Miguel Aon, Tian Xue, Ronald A. Li, Brian O'Rourke, Eduardo Marban. (2005). Anti-arrhythmic engineering of skeletal muscle myoblasts for cardiac transplantation. Circ. Res. 97(2): 159-67.

39. Suk-ying Tsang, Robert G. Tsushima, Gordon F. Tomaselli, Ronald A. Li, Peter H. Backx (2005) A multi-functional aromatic residue in the external pore vestibule of Na+ Channels contributes to the local anesthetic receptor. Mol. Pharm. 67(2):424-434.

40. Ezana Azene, Dongpei Sang, Suk-ying Tsang, Ronald A. Li (2005). Pore-to-gate coupling of pacemaker (HCN) channels revealed by a pore variant that contributes to gating but not permeation. Biochem Bioph Res Co. 327(4):1131-1142.

41. Tian, Xue, Heecheol, Cho, Fadi Akar, Suk-ying Tsang, Steven Jones, Eduardo Marbán, Gordon F. Tomaselli, Ronald A. Li (2005). Functional integration of electrically-active cardiac derivatives from genetically-engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: Insights into the development of cell-based pacemakers. Circulation. 111(1):11-20. See Comments 112(6):e82-83. Best Basic Study of 2005.

Reviews, Books & Chapters

1. Ellen Poon, Deborah K. Lieu, Ronald A. Li. 2011.. MicroRNA- and Pluripotent Stem Cell-based Heart Therapies: From the Electrophysiological Perspectives. Editor: Michael Sanguinetti.

2. Ellen Poon, Marco Kong, Ronald A. Li. 2011. Human pluripotent stem cells in cardiovascular research and regenerative medicine. Embryonic Stem Cells Book 2. ISBN 979-953-307-217-2

3. Fadi G. Akar, Ronald A. Li. 2011. Cell Therapy for Ventricular Arrhythmias & Ventricular Arrhythmias in Cell Therapy. Editor: Sam C. Dudley.

4. Ellen Poon, Marco Kong, Ronald A. Li. 2011. Pluripotent Stem Cell- and Gene-Based Approaches for Myocardial Repair. Mol Pharm. In press.

5. Rushing SN, Herren AW, Lieu DK, Ronald A. Li. (2011). RNAi Strategies for Genetic Manipulations of Human Embryonic Stem Cells for Myocardial Repair. METHODS IN MOLECULAR BIOLOGY. Editor: Nicole I. zur Nieden. Humana Press, NJ. Methods Mol Biol. 690:107-20.

6. Kong CW, Akar FG, Li RA. Translational potential of human embryonic and induced pluripotent stem cells for myocardial repair: insights from experimental models. Thromb Haemost. 2010 Jul 5;104(1):30-8. Epub 2010 Jun 10.

7. Ronald A. Li. 2008. Human Embryonic Stem Cells for Myocardial Repair. Editor: Jozef Anne. Springer Press, MA. Invited.

8. Ronald A. Li. Editor. 2008. The Sino-atrial Node: From Basic Research to Clinical Applications. Invited by Springer Press, MA.

9. Ronald A. Li, Jennifer Moore, Yelena Tarasova, Kenneth R. Boheler: Human Embryonic Stem Cell Derived Cardiomyocytes: Therapeutic Potential and Limitation. In Progress in Stem Cell Research, Edited by: P Koka, Nova Science Pub Inc, Hauppauge NY, United States, February 2008, pp. 149-169.

10. Ronald A. Li, Nipavan Chiamvimonvat (2007) Adrenergic Signaling and Cardiac Ion Channels: From Cell to Bedside. 5th Edition. Edited by Douglas P. Zipes and Jose Jalife. Philadelphia, Pa, Saunders.