Two-Photon Microscopy

Olympus FV1000 MPE

The Olympus FluoView FV1000MPE is a multi-photon laser scanning microscope that allows fluorescence imaging deep within specimens.  It has a Coherent Chameleon Vision II laser, tunable from 680nm to 1080nm, as well as a 473nm laser diode for single photon excitation.  It has four external (non-descan) detectors, as well as three confocal PMTs and one transmitted light detector.  It is capable of timelapse and FRET imaging experiments.  The motorized stage contains adapters for different dishes, slides, and small animals to accommodate a wide range of experimental setups, and is also capable of automated montage acquisition and stitching to generate high resolution images of large areas. Dipping lenses allow imaging directly into specimens in media.

 

Technical Specifications

Laser Lines

Detectors

Emission Filters

Dichroics

Internal IR Cut Filter

Scanning Modes

Bit Depth

Resolution

Ti:sapph (680-1080 nm)

CH1:

DAPI CFP

BA420-460

BA460-500

DM485LP

SDM505LP

SDM570LP

BA685RIF (692-1100 nm)

Frame

12

max: 4096 x 4096

LD 473

CH2:

GFP YFP

BA495-540HQ A520-560

 

DM485LP

SDM505LP

SDM570LP

 

Time Lapse

 

min: 64 x 64

 

CH3: WIDE DAPI GFP

A380-560

SDM570

 

Montage

 

 

 

CH4: RFP

BA575-630

SDM570

 

FRET

 

 

 

3 confocal PMTs

 

 

 

 

 

 

 

TD1

 

 

 

 

 

 

Objectives

Magnification

Immersion

NA

25x (dipping)*

water

1.05

10x (dipping)

water

0.3

20x (dipping)

water

0.95

40x (dipping)

water

0.8

60x (dipping)

water

0.9

100x (dipping)

water

1.0

10x

air

0.4

20x

air

0.75

40x

air

0.6

60x

oil

1.4

60x

water

1.2

User Protocol
Mosaic Protocol