Multi-Photon Microscopy

Olympus FluoView FV1000MPE

The Olympus FluoView FV1000MPE is newest addition to the Microscopy SRF funded by the NIH’s Shared Instrumentation Grant program. The FV1000 is a multi-photon laser scanning microscope that allows fluorescence imaging deep within specimens. It is the ideal microscope system for fluorescent imaging of thick tissue section, whole mount, or small animal imaging. It can also be used for visualizing collagen in tissue using second harmonic generation(SHG) imaging. Utilizing a Coherent Chameleon Vision II laser with pre-compensation, the FV1000 multi-photon system is able to image hundreds of microns into specimens thanks to the penetration of IR light, long working distance objectives, and it’s four external (non-descan) detectors. The Vision II laser tunable from 680nm to 1080nm. Single photon excitation can also be achieved on this system with a 473nm laser diode.