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Home > Resources > Shared Resource Facilities > Mouse Genetics and Gene Targeting > Protocols
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Shared Resource Facilities

  • BioMedical Engineering Resource
  • Flow Cytometry
  • Histology
  • Human Embryonic Stem Cell
  • In-Vivo Molecular Imaging
  • Irradiator
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  • Mouse Genetics and Gene Targeting
    • Overview
    • Protocols
    • Services
    • Request Forms
  • Quantitative PCR
  • Administration

Protocols

Transgene Purification for Microinjection [PDF](<20kb)

Tail DNA Purification for Genotyping [PDF]

ES Culture and Gene Targeting

  1. Preparation of Irradiated Primary Embryo Fibroblasts [PDF]
  2. Electroporation and Selection of ES Cells [PDF]
  3. Isolation, Growth and Analysis of ES Clones [PDF]
  4. Preparation of ES Clones for Blastocyst Injection [PDF]

 


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