Quantitative PCR Shared Resource Facility
The quantitative PCR Shared Resource Facility (qPCR SRF) provides access to fully automated instruments for Nanostring nCounter analysis, droplet digital PCR, real-time PCR, automated RNA/DNA isolation, and liquid handling. The facility offers services involving Nanostring nCounter analysis, PCR assay development, allelic discrimination assays, RNA/DNA extractions, cDNA synthesis, setting-up PCR reactions, and data analysis.
- The facility is equipped with two ABI PRISM 7900HT sequence detection systems, configured to use 384-well or 96-well plates with SYBR green or TaqMan probes and capable of unattended 24-hour operation using a robotic arm to process plates in the queue.
- The facility has a QX100 Droplet Digital PCR System. It has many potential applications, including the detection and quantification of low levels of pathogens, rare genetic sequences, copy number variations, relative gene expression in single cells, and analysis of heterogeneous DNA methylation.
- The facility provides access to the Nanostring Technologies nCounter platform that allows for highly multiplexed (up to 8000 targets per assay) digital quantification of nucleic acids.
- An eight channel 384/96-well format Beckman pipetting robot (Beckman Biomek NXp Laboratory Automation Workstation) is available for setting up PCR reactions in 384 and 96 well format.
- A Qiagen BioRobot Universal system is available for automated large-scale RNA and DNA isolation.
Visit eRAP to make a reservation for the facility equipment.
For funding purposes, it is important to acknowledge the qPCR Shared Resource Facility in all publications that include data derived from the facility.
Venugopalan Nair, PhD
Assistant Professor, Dept. of Neurology
For reservations, supplies, and appointments
Room 14-72, Box 1137
One Gustave L. Levy Place
New York, NY 10029