Benjamin R tenOever, PhD

• 2017
  Pew Innovator Award
  

• 2015
  Tocqueville Distinguished Chair Award
  

• 2013
  American Society of Virology Ann Palmenberg Award
  

• 2012
  Vilcek Award for Creative Promise
  

• 2012
  American Society of Gene and Cell Therapy Young Investigator Award
  

• 2011
  Cozzarelli Prize Recipient in Biomedical Sciences
  

• 2011
  Investigator in the Pathogenesis of Infectious Diseases
  

• 2010
  Young Investigator Award
  

• 2009
  Milstein Young Investigator Award
  

• 2008
  Presidential Early Career Award for Scientists and Engineers (PECASE)
  

• 2008
  Pew Scholar
  

Specific Clinical/Research Interests: Host-Pathogen Interactions

Current Students: Julie Eggenberger, Rasmus Moeller

Postdoctoral Fellows: Tristan Jordan, Daniel Melo-Blanco, Benjamin Nilsson

Research Personnel: Maryline Panis

Overview:
The tenOever lab is interested in the way cells have evolved to defend themselves against virus. More specifically, the lab focuses on what constitutes different cellular defense systems, how these systems have been shaped over time, and how viruses circumvent them and cause disease.

Perez JT, Pham AM, Lorini MH, Chua MA, Steel J, tenOever BR. MicroRNA-Mediated Species-Specific Attenuation of Influenza A Virus. Nat Biotechnol 2009 Jun; 27(6): 572-576.

Perez JT, Varble A, Sachidanandam R, Zlatev I, Manoharan M, García-Sastre A, tenOever BR. Influenza A virus-generated small RNAs regulate the switch from transcription to replication. Proceedings of the National Academy of Sciences of the United States of America 2010 Jun; 107(25).

Shapiro JS, Varble A, tenOever BR. Non-Canonical Cytoplasmic Processing of Viral microRNAs. RNA 2010; 16: 2068-74.

Varble A, Chua MA, Perez JT, Manicassamy B, Garcia-Sastre A, tenOever BR. Engineered RNA Viral Synthesis of microRNAs. PNAS 2010; 107(25): 11519-24.

Schmid S, Mordstein M, Kochs G, Garcia-Sastre A, tenOever BR. Transcription Factor Redundancy Ensures Induction of the Antiviral State. JBC 2010; 285(53): 42013-22.

Langlois RA, Shapiro JS, Pham AM, tenOever BR. In vivo delivery of cytoplasmic RNA virus-derived miRNAs. Molecular therapy : the journal of the American Society of Gene Therapy 2012 Feb; 20(2).

Pham AM, Langlois RA, tenOever BR. Replication in cells of hematopoietic origin is necessary for Dengue virus dissemination. PLoS pathogens 2012 Jan; 8(1).

Shapiro JS, Langlois RA, Pham AM, tenOever BR. Evidence for a cytoplasmic microprocessor of pri-miRNAs. RNA (New York, N.Y.) 2012 Jul; 18(7).

Langlois RA, Varble A, Chua MA, García-Sastre A, tenOever BR. Hematopoietic-specific targeting of influenza A virus reveals replication requirements for induction of antiviral immune responses. Proceedings of the National Academy of Sciences of the United States of America 2012 Jul; 109(30).

Backes S, Shapiro JS, Sabin LR, Pham AM, Reyes I, Moss B, Cherry S, tenOever BR. Degradation of host microRNAs by poxvirus poly(A) polymerase reveals terminal RNA methylation as a protective antiviral mechanism. Cell host & microbe 2012 Aug; 12(2).

Perez JT, Zlatev I, Aggarwal S, Subramanian S, Sachidanandam R, Kim B, Manoharan M, tenOever BR. A small-RNA enhancer of viral polymerase activity. Journal of virology 2012 Dec; 86(24).

Chua MA, Schmid S, Perez JT, Langlois RA, tenOever BR. Influenza A Virus Utilizes Suboptimal Splicing to Coordinate the Timing of Infection. Cell reports 2013 Jan;.

tenOever BR. RNA viruses and the host microRNA machinery. Nature reviews. Microbiology 2013 Mar; 11(3).

Langlois RA, Albrecht RA, Kimble B, Sutton T, Shapiro JS, Finch C, Angel M, Chua MA, Gonzalez-Reiche AS, Xu K, Perez D, García-Sastre A, tenOever BR. MicroRNA-based strategy to mitigate the risk of gain-of-function influenza studies. Nature biotechnology 2013 Sep; 31(9).

Varble A, Benitez AA, Schmid S, Sachs D, Shim JV, Rodriguez-Barrueco R, Panis M, Crumiller M, Silva JM, Sachidanandam R, tenOever BR. An in vivo RNAi screening approach to identify host determinants of virus replication. Cell host & microbe 2013 Sep; 14(3).

Cullen BR, Cherry S, tenOever BR. Is RNA interference a physiologically relevant innate antiviral immune response in mammals?. Cell host & microbe 2013 Oct; 14(4).

Schmid S, Sachs D, tenOever BR. Mitogen-activated Protein Kinase-mediated Licensing of Interferon Regulatory Factor 3/7 Reinforces the Cell Response to Virus. The Journal of biological chemistry 2014 Jan; 289(1).

Schmid S, Zony LC, tenOever BR. A versatile RNA vector for delivery of coding and non-coding RNAs. Journal of Virology 2013 Dec;.

Han J, Perez JT, Chen C, Li Y, Benitez A, Kandasamy M, Lee Y, Andrade J, tenOever B, Manicassamy B. Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Cell reports 2018 Apr; 23(2).

Aguado LC, tenOever BR. RNase III Nucleases and the Evolution of Antiviral Systems. BioEssays : news and reviews in molecular, cellular and developmental biology 2018 Feb; 40(2).

Wohlbold TJ, Podolsky KA, Chromikova V, Kirkpatrick E, Falconieri V, Meade P, Amanat F, Tan J, tenOever BR, Tan GS, Subramaniam S, Palese P, Krammer F. Broadly protective murine monoclonal antibodies against influenza B virus target highly conserved neuraminidase epitopes. Nature microbiology 2017 Oct; 2(10).

Duehr J, Wohlbold TJ, Oestereich L, Chromikova V, Amanat F, Rajendran M, Gomez-Medina S, Mena I, tenOever BR, García-Sastre A, Basler CF, Munoz-Fontela C, Krammer F. Novel Cross-Reactive Monoclonal Antibodies against Ebolavirus Glycoproteins Show Protection in a Murine Challenge Model. Journal of virology 2017 08; 91(16).

tenOever BR. Questioning antiviral RNAi in mammals. Nature microbiology 2017 Apr; 2.

Beaty SM, Park A, Won ST, Hong P, Lyons M, Vigant F, Freiberg AN, tenOever BR, Duprex WP, Lee B. Efficient and Robust Reverse Genetics Systems. mSphere; 2(2).

Morales L, Oliveros JC, Fernandez-Delgado R, tenOever BR, Enjuanes L, Sola I. SARS-CoV-Encoded Small RNAs Contribute to Infection-Associated Lung Pathology. Cell host & microbe 2017 Mar; 21(3).

Aguado LC, Schmid S, May J, Sabin LR, Panis M, Blanco-Melo D, Shim JV, Sachs D, Cherry S, Simon AE, Levraud JP, tenOever BR. RNase III nucleases from diverse kingdoms serve as antiviral effectors. Nature 2017 07; 547(7661).

Moy RH, Cole BS, Yasunaga A, Gold B, Shankarling G, Varble A, Molleston JM, tenOever BR, Lynch KW, Cherry S. Stem-loop recognition by DDX17 facilitates miRNA processing and antiviral defense. Cell 2014 Aug; 158(4).

Backes S, Langlois RA, Schmid S, Varble A, Shim JV, Sachs D, tenOever BR. The Mammalian response to virus infection is independent of small RNA silencing. Cell reports 2014 Jul; 8(1).

Thapa RJ, Ingram JP, Ragan KB, Nogusa S, Boyd DF, Benitez AA, Sridharan H, Kosoff R, Shubina M, Landsteiner VJ, Andrake M, Vogel P, Sigal LJ, tenOever BR, Thomas PG, Upton JW, Balachandran S. DAI Senses Influenza A Virus Genomic RNA and Activates RIPK3-Dependent Cell Death. Cell host & microbe 2016 Nov; 20(5).

Moy RH, Cole BS, Yasunaga A, Gold B, Shankarling G, Varble A, Molleston JM, tenOever BR, Lynch KW, Cherry S. Stem-loop recognition by DDX17 facilitates miRNA processing and antiviral defense. Cell 2014 Aug; 158(4).

Rajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Martínez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, García-Sastre A. Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKKε kinase-mediated antiviral response. Immunity 2014 Jun; 40(6).

Shapiro JS, Schmid S, Aguado LC, Sabin LR, Yasunaga A, Shim JV, Sachs D, Cherry S, tenOever BR. Drosha as an interferon-independent antiviral factor. Proceedings of the National Academy of Sciences of the United States of America 2014 May; 111(19).

Benitez AA, Spanko LA, Bouhaddou M, Sachs D, tenOever BR. Engineered Mammalian RNAi Can Elicit Antiviral Protection that Negates the Requirement for the Interferon Response. Cell reports 2015 Nov; 13(7).

Benitez AA, Panis M, Xue J, Varble A, Shim JV, Frick AL, López CB, Sachs D, tenOever BR. In Vivo RNAi Screening Identifies MDA5 as a Significant Contributor to the Cellular Defense against Influenza A Virus. Cell reports 2015 Jun; 11(11).

Varble A, Albrecht RA, Backes S, Crumiller M, Bouvier NM, Sachs D, García-Sastre A, tenOever BR. Influenza A virus transmission bottlenecks are defined by infection route and recipient host. Cell host & microbe 2014 Nov; 16(5).

tenOever BR. Response to Voinnet et al. Cell reports 2014 Nov; 9(3).

Aguado LC, Schmid S, Sachs D, Shim JV, Lim JK, tenOever BR. microRNA Function Is Limited to Cytokine Control in the Acute Response to Virus Infection. Cell host & microbe 2015 Dec; 18(6).

tenOever BR. The Evolution of Antiviral Defense Systems. Cell host & microbe 2016 Feb; 19(2).

Laurent-Rolle M, Morrison J, Rajsbaum R, Macleod JM, Pisanelli G, Pham A, Ayllon J, Miorin L, Martinez C, tenOever BR, García-Sastre A. The interferon signaling antagonist function of yellow fever virus NS5 protein is activated by type I interferon. Cell host & microbe 2014 Sep; 16(3).

Heaton NS, Langlois RA, Sachs D, Lim JK, Palese P, tenOever BR. Long-term survival of influenza virus infected club cells drives immunopathology. The Journal of experimental medicine 2014 Aug; 211(9).