Carmen Argmann, PhD
- ASSOCIATE PROFESSOR | Genetics and Genomic Sciences
Dr. Argmann has a doctorate from the faculty of Science at the University of Western Ontario, Canada where she showed that PPARγ and LXR activation could dramatically reduce macrophage foam cell formation, a key event in atherosclerosis. During her postdoctoral studies at the Institut de Génétique et de Biologie Moléculaire et Cellulaire in Strasbourg, France she contributed to the development of high-throughput mouse metabolic phenotyping protocols and demonstrated that resveratrol, a compound found in red wine, improves mitochondrial function and protects against metabolic disease in vivo.
As a research scientist in Dr Schadt’s genetics group at Rosetta Inpharmatics she contributed to the designing of large-scale genetic mouse crosses to address novel facets of metabolic disease. She was involved in integrating DNA variation, gene expression, and clinical data collected, in order to uncover core networks associated with metabolic disease processes, which in turn were used to identify novel therapeutic targets for the Diabetes and Obesity franchise.
In 2010, during her time in Dr. Aerts’s lab at the Academic Medical Center of the University of Amsterdam, she developed further into an integrative biologist. Her main focus has become applying novel integrative systems biology approaches to understand the hallmarks and key drivers of various human diseases.
Since 2013 she is an assistant professor at Mount Sinai in the Institute for Genomics and Multiscale Biology and is actively applying her integrative biology approaches in various large scale collaborations associated with: generating network models in inflammatory bowel disease; finding novel human beta cell regeneration strategies for Type 2 Diabetes and uncovering genetic modifiers of screenable inborn errors of metabolism.
Figure: Example schema of the integrative approaches applied to understanding disease processes.
Currently we have one postdoctoral position available. Please contact me at email@example.com
Multi-Disciplinary Training AreaGenetics and Data Science [GDS]
PhD, University of Western Ontario
Identifying novel biological insights on complex human diseases requires spanning both the biological and computational worlds. Our biological data mining strategies aim to do this by helping to ask the right computational questions in order to get the right biological answers.
One major part of our group’s research is to facilitate generation of large scale datasets which can be integrated into causal predictive molecular networks which we then interpret for the pathophysiology of underlying complex biological questions. Our network insights are refined into candidate genes and pathways and formulated into testable hypotheses, some of which we perform experimental validation of ourselves.
Ultimately we aim to use this knowledge to predict novel therapeutic candidates for diseases of interest. We have applied these methods in multiple disciplines covering complex diseases (metabolic syndrome, IBD and cancer), complex traits (aging) as well as inborn errors of metabolism (Gaucher’s disease and Mitochondrial disorders).
Our system biology approaches are highly collaborative projects as they require various expertise from clinicians in the clinic, to experimentalist in the wet lab to the computational analyst in the dry lab. Three of my group’s main projects are summarized:
Project 1: Innovative data-driven multiscale biology approaches to discover modifiers of screenable disorders in newborns
Background: Inborn errors of metabolism (IEM) are increasingly viewed as complex diseases as they often present as a spectrum of disease phenotypes with a clear disconnect between the severity of mutation at the primary affected locus and the phenotype. The lack of genotype and phenotype correlation greatly impacts the ability to predict a patient’s disease course. It also illustrates the existence of a fundamental gap in our knowledge of disease pathophysiology. The era of one-gene one-disease is being abandoned, and the contribution of modifying factors considered. However, identifying the modifying factors is not trivial, as rare diseases have rare data.
Hypothesis: We hypothesize that by embracing the concept of IEM as complex diseases that 1. datasets and 2. network approaches generated in populations with common disorders can be used to study disease modifying biology in IEM thereby overcoming the rare disease rare data drawback. Our strategy is based on observing that differentially expressed genes from IEM experimental models highlight highly connected subnetworks in the molecular networks established in common disease populations.
Aim: Our lab’s aim is to use our innovative data-driven multi-scale computational approach to derive and then wet-lab validate novel candidate modifying genes and their associated biology related to the screenable inborn errors of fatty acid oxidation (FAO) the lysosomal storage disorder, Gaucher disease (GD). This is a key collaboration between myself and a faculty expert in IEM, Dr Sander Houten, also of the Department of Genetics and Genomics Sciences at ICAHN.
Impact: Combined these two aims will break new ground for FAO and GD and rare diseases in general by overcoming inherent limitations of rare data through combining novel methodologies with existing data. We furthermore perform validations and these methods which could point the IEM research field into multiple new directions. These novel insights are needed to propel the IEM field into the next generation of understanding.
Project 3: Generating predictive network models for Inflammatory Bowel Disease (IBD):
Background: Inflammatory bowel disease (IBD) involves chronic inflammation of all or part of your digestive tract and primarily includes ulcerative colitis and Crohn's disease. IBD can be debilitating with severe diarrhea, pain, fatigue and weight loss and is sometimes associated with life-threatening complications. Currently there is an unmet therapeutic need with many patients either non-responsive or refractory to current treatment options. MSSM from the clinic, to preclinical models to computational modelers have extensive expertise in IBD. Because of this expertise Janssen pharmaceuticals in 2013 entered into a multi-million dollar collaboration with MSSM clinicians, preclinical scientists and computational biologists to generate new patient information, understand preclinical IBD mouse models and computational interpret all the data as predictive networks of IBD.
Hypothesis: The underlying hypothesis we are testing is that the through multi-scale modeling of IBD patient derived molecular insights that we can better understand the disease pathology and predict candidate novel genes and biology for potential therapeutic development. I have been involved in various aspects of this collaboration using my integrative biologist skill set to help generate data for and interpret the networks.
Impact: Data driven models have proven effective in understanding other complex diseases (Diabetes, obesity CVD) thus the application of these models to IBD is a much needed complementary approach to interpreting a very complex disease process.
For a complete list of Dr. Argmann's publications http://www.ncbi.nlm.nih.gov/pubmed/?term=argmann+C
Project 2: Learning about human beta cell regeneration by integrating molecular landscapes of human insulinomas
Background: Adult human beta cells have proven remarkably resistant to therapeutic replication and expansion. This has significantly limited therapeutic options for Type 1 and 2 diabetics, both types which result entirely or in part from a deficiency of normal insulin-producing pancreatic beta cells.
Hypothesis/Aims: This knowledge gap has prompted an extensive search for strategies to regenerate or replace lost or dysfunctional beta cells. An intriguing, unexplored proliferative model is insulinoma, rare tumors of the adult beta cell. With this rationale, Dr Andrew Stewart (Director of the Diabetes and Obesity Institute at MSSM) initiated a collaboration with myself, Dr Bojan Losic (MSSM GGS faculty) and Dr Eric Schadt (Director of the Icahn Institute for Multi-Scale Biology and GGS department) in order to perform the first comprehensive genomic and transcriptomic characterization of human insulinomas. We are then integrating these molecular landscapes with those of normal beta cell transcriptomes in order to reveal underlying biological processes in insulinomas including proliferation mechanisms. Several predicted cell cycle gene candidates are being experimentally confirmed.
Impact: Overall our integrative approach hopes to uncover the key hallmarks and drivers of human insulinoma which distinguish them from normal beta cells and may serve as therapeutic targets for the diabetes field.
Tol MJ, Ottenhoff R, van Eijk M, Zelcer N, Aten J, Houten SM, Geerts D, van Roomen C, Bierlaagh MC, Scheij S, Hoeksema MA, Aerts JM, Bogan JS, Dorn GW, Argmann CA, Verhoeven AJ. A PPARγ-Bnip3 axis couples adipose mitochondrial fusion-fission balance to systemic insulin sensitivity. Diabetes 2016 Jun;.
Williams EG, Wu Y, Jha P, Dubuis S, Blattmann P, Argmann CA, Houten SM, Amariuta T, Wolski W, Zamboni N, Aebersold R, Auwerx J. Systems proteomics of liver mitochondria function. Science (New York, N.Y.) 2016 Jun; 352(6291).
Long Q, Argmann C, Houten SM, Huang T, Peng S, Zhao Y, Tu Z, Zhu J. Inter-tissue coexpression network analysis reveals DPP4 as an important gene in heart to blood communication. Genome medicine 2016; 8(1).
Argmann CA, Houten SM, Zhu J, Schadt EE. A Next Generation Multiscale View of Inborn Errors of Metabolism. Cell metabolism 2016 Jan; 23(1).
Wu Y, Williams EG, Dubuis S, Mottis A, Jovaisaite V, Houten SM, Argmann CA, Faridi P, Wolski W, Kutalik Z, Zamboni N, Auwerx J, Aebersold R. Multilayered genetic and omics dissection of mitochondrial activity in a mouse reference population. Cell 2014 Sep; 158(6).
Houtkooper RH, Argmann C, Houten SM, Cantó C, Jeninga EH, Andreux PA, Thomas C, Doenlen R, Schoonjans K, Auwerx J. The metabolic footprint of aging in mice. Scientific reports 2011; 1.
Argmann C, Dobrin R, Heikkinen S, Auburtin A, Pouilly L, Cock TA, Koutnikova H, Zhu J, Schadt EE, Auwerx J. Ppargamma2 is a key driver of longevity in the mouse. PLoS genetics 2009 Dec; 5(12).
Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, Geny B, Laakso M, Puigserver P, Auwerx J. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell 2006 Dec; 127(6).
Di Narzo AF, Peters LA, Argmann C, Stojmirovic A, Perrigoue J, Li K, Telesco S, Kidd B, Walker J, Dudley J, Cho J, Schadt EE, Kasarskis A, Curran M, Dobrin R, Hao K. Blood and Intestine eQTLs from an Anti-TNF-Resistant Crohn's Disease Cohort Inform IBD Genetic Association Loci. Clinical and translational gastroenterology 2016; 7(6).
Houten SM, Herrema H, Te Brinke H, Denis S, Ruiter JP, van Dijk TH, Argmann CA, Ottenhoff R, Müller M, Groen AK, Kuipers F, Reijngoud DJ, Wanders RJ. Impaired amino acid metabolism contributes to fasting-induced hypoglycemia in fatty acid oxidation defects. Human molecular genetics 2013 Dec; 22(25).
Wegdam W, Argmann CA, Kramer G, Vissers JP, Buist MR, Kenter GG, Aerts JM, Meijer D, Moerland PD. Label-free LC-MSe in tissue and serum reveals protein networks underlying differences between benign and malignant serous ovarian tumors. PloS one 2014; 9(9).