Hugh A Sampson, MD
- PROFESSOR | Pediatrics, Allergy and Immunology
Specialties:Pediatrics, Pediatric Allergy and Immunology
Research Topics:Allergy, B Cells, Immunological Tolerance, T Cells
Dr. Hugh Sampson is the Kurt Hirschhorn Professor of Pediatrics at the Icahn School of Medicine at Mount Sinai and Director Emeritus of the Jaffe Food Allergy Institute. Dr. Sampson's research interests have focused on food allergic disorders, and now include work on the pathogenesis of food-induced anaphylaxis, characterization of allergenic food proteins and their processing by the immune system, genetics of food allergy, development of novel diagnostic tests, and mechanisms of immunotherapeutic strategies for treating food allergies including basic studies and clinical trials in oral, sublingual and epicutaneous immunotherapy and the potential use of biologics, such as anti-IgE and anti-cytokine monoclonal antibodies. His research is funded by a number of grants from the National Institutes of Health, including the recently funded Exposure to Vaginal Microbiome in C-section Infants at High-risk for Allergies – A Pilot Study. Dr. Sampson supervises one clinic/week in pediatric allergy and is involved in teaching fellows and residents. He is an Associate Editor of Pediatric Allergy & Immunology, past-president of the American Academy of Allergy, Asthma & Immunology, and a member of the National Academy of Medicine.
In the News
Dr. Sampson talks about food allergies in The Daily News featureThe Daily Check Up.
Dr. Sampson discusses childhood food allergies in The Daily News feature The Daily Check Up.
American Board of Allergy & Immunology
American Board of Pediatrics
Multi-Disciplinary Training AreasImmunology [IMM], Public Health [PH]
MD, S.U.N.Y., Buffalo
Residency, Pediatrics, Children's Memorial Hospital
Fellowship, Allergy & Immunology, Duke University Hospital
Brett Ratner Award
Outstanding Research in Food Hypersensitivity Disorders
International Award in Nutrition
New York Top Doctors
Allergic Disease Academic Award
Specific Clinical/Research Interest: Immunopathogenic mechanisms of food allergic disorders and asthma; immunomodulatory therapies
Postdoctoral Fellows: Jacob Kattan, MD
Research Personnel: Alexander Grishin, PhD; Madhan Masilamani, PhD, Jing S Lin, PhD, Luda Bardina, MS; Galina Grishin, MS; Gustavo Gimenez, BS, Mohanapriya Kamalakannan, BS
Summary of Research Studies:
Our laboratory is evaluating immunopathologic mechanisms of food allergic disorders. Specifically we are identifying allergenic proteins at a molecular and structural level, and investigating the interaction between IgE antibodies and allergenic proteins and the immune response at a cellular and molecular level. Studies utilize patient specimens and murine models in an attempt to elucidate underlying mechanisms. Allergenic proteins in egg, milk, peanut, several tree nuts, shrimp and some fish have been fully characterized and full-length cDNAs isolated and cloned. A number of therapeutic strategies are under investigation utilizing murine models of anaphylaxis and asthma including the use of recombinant proteins, DNA vaccines, and CpG-conjugated proteins. Several early stage, human clincial trials are in progress to treat food allergic disorders including oral and sublingual immunotherapy, herbal therapies, and engineered recombinant protein vaccines.
In addition, our laboratory is serving as the mechanistic center for the NIAID Inner City Asthma Consortium and the Consortiium for Food Allergy Research. The asthma consortium is investigating the role of allergic sensitization in inner city children and its potential role in the increased morbidity and mortality found in this population. The overall goal is to determine whether the nature and quantity of environmental allergens within the inner city, especially cockroach, are unique in their ability to determine and drive the intensity of allergic inflammation in sensitized children residing in the inner city and thus the severity of their asthma.
The food allergy consortium is investigating the immunologic mechanisms associated with the devleopment of peanut allergy and the development of tolerance ["outgrowing"] to egg and milk allergy, the immunologic consequences of oral immunotherapy for egg allergy, and the immunologic consequences of sublingual immunotherapy for peanut allergy. The lab employs a variety of techniques to identify and purify proteins including SDS-PAGE, immunoblotting and HPLC. Recombinant proteins are generated from cDNA isolated from appropriate cDNA libraries. A variety of techniques are utilized to study both humoral and cellular responses of patient groups and controls. Characterization of cellular responses includes intracytoplasmic staining, mRNAgeneration, and characterization of cytokines secreted into cell supernatants. Similar studies are conducted in the murine models.
Jaffe Food Allergy Institute
Shreffler WG, Lencer DA, Bardina L, Sampson HA. IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2. J Allergy Clin Immunol 2005; 116: 893-899.
Jarvinen KM, Beyer K, Vila L, Bardina L, Mishoe M, Cooke SH, Sampson HA. Specificity of IgE antibodies to linear epitopes of hen's egg ovomucoid as a marker for persistence of egg allergy. Allergy 2007; 62: 758-765.
Beyer K, Grishina G, Bardina L, Sampson HA. Identification of two new sesame seed allergens -Ses i 6 and Ses i 7. J Allergy Clin Immunol 2007; 119: 1554-1556.
Chehade M, Sampson HA, Morotti RA, Magid MS. Esophageal subepithelial fibrosis in children with eosinophilic esophagitis. J Pediatr Gastroenterol Nutr 2007 Sep; 45(3): 319-328.
Han N, Jarvinen KM, Cocco RR, Busse PJ, Sampson HA, Beyer K. Identification of amino acids critical for IgE-binding to sequential epitopes of bovine kappa-casein and the similarity of these epitopes to the corresponding human kappa-casein sequence. Allergy 2008 Feb; 63(2): 198-204.
Jarvinen KM, Han N, Cocco R, Busse PJ, Sampson HA, Beyer K. Identification of Amino Acids Critical for IgE-binding to Bovine-Casein and Potential homology between of IgE-binding Epitopes of Cow's Milk Caseins and Caseins of Man. Allergy 2008; 63(2): 198-204.
Nowak-Wegrzyn A, Bloom KA, Sicherer SH, Shreffler WG, Noone S, Wanich N, Sampson HA. Tolerance to extensively heated milk in children with cow's milk allergy. J Allergy Clin Immunol 2008 Aug; 122(2): 342-347.
Cerecedo I, Zamora J, Shreffler WG, Lin J, Bardina L, Dieguez MC, Wang J, Muriel A, de la Hoz B, Sampson HA. Mapping of the IgE and IgG4 sequential epitopes of milk allergens with a peptide microarray-based immunoassay. J Allergy Clin Immunol 2008 Sep; 122(3): 589-594.
Lemon-Mule H, Sampson HA, Sicherer SH, Shreffler WG, Noone S, Nowak-Wegrzyn A. Immunologic changes in children with egg allergy ingesting extensively heated egg. J Allergy Clin Immunol 2008 Nov; 122(5): 977-983.
Shreffler WG, Wanich N, Maloney M, Nowak-Wegrzyn A, Sampson HA. The Association of Allergen-Specific Regulatory T Cells with the Onset of Clinical Tolerance to Milk Protein. J Allergy Clin Immunol 2009 Jan; 123(1): 43-52.
Pochard P, Vickery B, Berin MC, Grishin A, Sampson HA, Caplan M, Bottomly K. Targeting Toll-like receptors on dendritic cells modifies the T(H)2 response to peanut allergens in vitro. The Journal of allergy and clinical immunology 2010 Jul; 126(1).
Konstantinou GN, Ramon B, Grishin A, Caubet JC, Bardina L, Sicherer SH, Sampson HA, Nowak-Wegrzyn A. The role of casein-specific IgA and TGF-beta in children with Food Protein-Induced Enterocolitis Syndrome to milk. Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 2014 Oct;.
Tordesillas L, Goswami R, Benede S, Grishina G, Dunkin D, Jarvinen KM, Maleki SJ, Sampson HA, Berin MC. Skin exposure promotes a Th2-dependent sensitization to peanut allergens. The Journal of clinical investigation 2014 Oct;.
Simons FE, Sampson HA. Anaphylaxis: Unique aspects of clinical diagnosis and management in infants (birth to age 2 years). The Journal of allergy and clinical immunology 2014 Oct;.
Järvinen KM, Suárez-Fariñas M, Savilahti E, Sampson HA, Berin MC. Immune factors in breast milk related to infant milk allergy are independent of maternal atopy. The Journal of allergy and clinical immunology 2014 Dec;.
Lee TD, Gimenez G, Grishina G, Mishoe M, Sampson HA, Bunyavanich S. Profile of a milk-allergic patient who tolerated partially hydrolyzed whey formula. The journal of allergy and clinical immunology. In practice 2015 Jan-Feb; 3(1).
Burks AW, Wood RA, Jones SM, Sicherer SH, Fleischer DM, Scurlock AM, Vickery BP, Liu AH, Henning AK, Lindblad R, Dawson P, Plaut M, Sampson HA. Sublingual immunotherapy for peanut allergy: Long-term follow-up of a randomized multicenter trial. The Journal of allergy and clinical immunology 2015 Feb;.
Gruchalla RS, Sampson HA. Preventing peanut allergy through early consumption--ready for prime time?. The New England journal of medicine 2015 Feb; 372(9).
Pascal M, Grishina G, Yang AC, Sánchez-García S, Lin J, Towle D, Ibañez MD, Sastre J, Sampson HA, Ayuso R. Molecular Diagnosis of Shrimp Allergy: Efficiency of Several Allergens to Predict Clinical Reactivity. The journal of allergy and clinical immunology. In practice 2015 Mar;.
Sampson HA. Historical background, definitions and differential diagnosis. Chemical immunology and allergy 2015 May; 101.
Gruchalla RS, Sampson HA. Peanut consumption in infants at risk for peanut allergy. The New England journal of medicine 2015 May; 372(22).
Wang J, Jones SM, Pongracic JA, Song Y, Yang N, Sicherer SH, Makhija MM, Robison RG, Moshier E, Godbold J, Sampson HA, Li XM. Safety, clinical, and immunologic efficacy of a Chinese herbal medicine (Food Allergy Herbal Formula-2) for food allergy. The Journal of allergy and clinical immunology 2015 Jun;.
Chang LM, Song Y, Li XM, Sampson HA, Masilamani M. Dietary Elimination of Soybean Components Enhances Allergic Immune Response to Peanuts in BALB/c Mice. International archives of allergy and immunology 2015 Jun; 166(4).
Kattan JD, Sampson HA. Clinical reactivity to soy is best identified by component testing to Gly m 8. The journal of allergy and clinical immunology. In practice 2015 Jul;.
Goldberg MR, Nachshon L, Appel MY, Elizur A, Levy MB, Eisenberg E, Sampson HA, Katz Y. Efficacy of baked milk oral immunotherapy in baked milk-reactive allergic patients. The Journal of allergy and clinical immunology 2015 Jul;.
Andreae DA, Grishina G, Sackesen C, Ibáñez MD, Sampson HA. High similarity between lentil and other lentil-like-proteins (dal) complicates recommendations on avoidance in lentil allergic patients. The journal of allergy and clinical immunology. In practice 2015 Jul;.
Wood RA, Kim JS, Lindblad R, Nadeau K, Henning AK, Dawson P, Plaut M, Sampson HA. A randomized, double-blind, placebo-controlled study of omalizumab combined with oral immunotherapy for the treatment of cow's milk allergy. The Journal of allergy and clinical immunology 2015 Oct;.
Fleischer DM, Sampson HA. Reply. The Journal of allergy and clinical immunology 2015 Nov;.
Fleischer DM, Sampson HA. Reply. The Journal of allergy and clinical immunology 2015 Nov;.
Schoos AM, Kattan JD, Gimenez G, Sampson HA. Sensitization phenotypes based on protein groups and associations to allergic diseases in children. The Journal of allergy and clinical immunology 2015 Nov;.
Gruchalla RS, Sampson HA, Liu AH, Shreffler W, Wallace PK, Togias A, David G, Calatroni A, LeBeau P. Effects of omalizumab on T lymphocyte function in inner-city children with asthma. Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 2015 Nov;.
Järvinen KM, Westfall J, De Jesus M, Mantis NJ, Carroll JA, Metzger DW, Sampson HA, Berin MC. Role of Maternal Dietary Peanut Exposure in Development of Food Allergy and Oral Tolerance. PloS one 2015 Dec; 10(12).
Sicherer SH, Wood RA, Vickery BP, Perry TT, Jones SM, Leung DY, Blackwell B, Dawson P, Burks AW, Lindblad R, Sampson HA. Impact of Allergic Reactions on Food-Specific IgE Concentrations and Skin Test Results. The journal of allergy and clinical immunology. In practice 2015 Dec;.
Gregory JA, Shepley-McTaggart A, Umpierrez M, Hurlburt BK, Maleki SJ, Sampson HA, Mayfield SP, Berin MC. Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice. Plant biotechnology journal 2016 Jan;.
Goodman RE, Ebisawa M, Ferreira F, Sampson HA, van Ree R, Vieths S, Baumert JL, Bohle B, Lalithambika S, Wise J, Taylor SL. AllergenOnline: A peer-reviewed, curated allergen database to assess novel food proteins for potential cross-reactivity. Molecular nutrition & food research 2016 Feb;.
Jones SM, Burks AW, Keet C, Vickery BP, Scurlock AM, Wood RA, Liu AH, Sicherer SH, Henning AK, Lindblad RW, Dawson P, Berin C, Fleischer DM, Leung DY, Plaut M, Sampson HA. Long-term treatment with egg oral immunotherapy enhances sustained unresponsiveness that persists after cessation of therapy. The Journal of allergy and clinical immunology 2016 Feb;.
Kamalakannan M, Chang LM, Grishina G, Sampson HA, Masilamani M. Identification and characterization of DC-SIGN-binding glycoproteins in allergenic foods. Allergy 2016 Mar;.
Ramesh M, Yuenyongviwat A, Konstantinou GN, Lieberman J, Pascal M, Masilamani M, Sampson HA. Peanut T-cell epitope discovery: Ara h 1. The Journal of allergy and clinical immunology 2016 Mar;.
Wright BL, Kulis M, Orgel KA, Burks AW, Dawson P, Henning AK, Jones SM, Wood RA, Sicherer SH, Lindblad RW, Stablein D, Leung DY, Vickery BP, Sampson HA. Component-resolved analysis of IgA, IgE, and IgG4 during egg OIT identifies markers associated with sustained unresponsiveness. Allergy 2016 Mar;.
Srivastava KD, Siefert A, Fahmy TM, Caplan MJ, Li XM, Sampson HA. Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy. The Journal of allergy and clinical immunology 2016 Apr;.
Han Y, Lin J, Bardina L, Grishina GA, Lee C, Seo WH, Sampson HA. What Characteristics Confer Proteins the Ability to Induce Allergic Responses? IgE Epitope Mapping and Comparison of the Structure of Soybean 2S Albumins and Ara h 2. Molecules (Basel, Switzerland) 2016 May; 21(5).
Tordesillas L, Mondoulet L, Blazquez AB, Benhamou PH, Sampson HA, Berin MC. Epicutaneous immunotherapy induces gastrointestinal LAP(+) regulatory T cells and prevents food-induced anaphylaxis. The Journal of allergy and clinical immunology 2016 Jun;.
Smith PK, Masilamani M, Li XM, Sampson HA. The false alarm hypothesis: Food allergy is associated with high dietary advanced glycation end-products and proglycating dietary sugars that mimic alarmins. The Journal of allergy and clinical immunology 2016 Jul;.
Caubet JC, Bencharitiwong R, Ross A, Sampson HA, Berin MC, Nowak-Węgrzyn A. Humoral and cellular responses to casein in patients with food protein-induced enterocolitis to cow's milk. The Journal of allergy and clinical immunology 2016 Jul;.
Ungar B, Correa da Rosa J, Shemer A, Czarnowicki T, Estrada YD, Fuentes-Duculan J, Xu H, Zheng X, Peng X, Suárez-Fariñas M, Nowak-Wegrzyn A, Sampson HA, Krueger JG, Guttman-Yassky E. Patch testing of food allergens promotes Th17 and Th2 responses with increased IL-33: A pilot study. Experimental dermatology 2016 Aug;.
Tordesillas L, Rahman AH, Hartmann BM, Sampson HA, Berin MC. Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut. The Journal of allergy and clinical immunology 2016 Aug;.
Sampson HA. Food allergy: Past, present and future. Allergology international : official journal of the Japanese Society of Allergology 2016 Sep;.