Jean K Lim, PhD
- ASSOCIATE PROFESSOR | Microbiology
Research Topics:Biodefense, Cellular Immunity, Chemokines, Immunology, Infectious Disease, Inflammation, Trafficking, Viruses and Virology
The Lim lab focuses on understanding the pathogenesis of vector-borne viruses, such as West Nile virus (WNV) and how cell trafficking controls viral spread and disease. West Nile virus is a highly virulent re-emerging human pathogen and the leading cause of arthropod-borne encephalitis in the US. Neither vaccines nor antiviral or immunomodulator therapies are available for WNV, and new concepts based on precise understanding of molecular pathogenesis are needed. WNV causes meningitis and multifocal encephalitis in both human disease and mouse models, characterized by the accumulation of leukocytes, including monocytes and T cells, in the brain. The major research projects in the lab are to 1) understand leukocyte trafficking events, both at the molecular and cellular level, as they relate to host defense against WNV, 2) understand host genetic susceptibility to WNV by evaluating WNV-infected human samples; and 3) develop new models to evaluate WNV and other flavivirus infection. The primary tools used to evaluate these questions are a well-established mouse model of WNV infection, samples acquired from WNV-infected individuals, and an ex vivo model of WNV infection in human lymphoid tissue. A combination of these approaches will provide a comprehensive understanding of WNV pathogenesis and virus-host interactions.
Watch a video featuring the Microbiology and Virology PhD Graduate School Program.
Multi-Disciplinary Training AreasImmunology [IMM], Microbiology [MIC]
NIAID, National Institutes of Health
PhD, University of Maryland
Aguado LC, Schmid S, Sachs D, Shim JV, Lim JK, tenOever BR. microRNA Function Is Limited to Cytokine Control in the Acute Response to Virus Infection. Cell host & microbe 2015 Dec; 18(6).
Drummond RA, Collar AL, Swamydas M, Rodriguez CA, Lim JK, Mendez LM, Fink DL, Hsu AP, Zhai B, Karauzum H, Mikelis CM, Rose SR, Ferre EM, Yockey L, Lemberg K, Kuehn HS, Rosenzweig SD, Lin X, Chittiboina P, Datta SK, Belhorn TH, Weimer ET, Hernandez ML, Hohl TM, Kuhns DB, Lionakis MS. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS pathogens 2015 Dec; 11(12).
Bardina SV, Michlmayr D, Hoffman KW, Obara CJ, Sum J, Charo IF, Lu W, Pletnev AG, Lim JK. Differential Roles of Chemokines CCL2 and CCL7 in Monocytosis and Leukocyte Migration during West Nile Virus Infection. Journal of immunology (Baltimore, Md. : 1950) 2015 Nov; 195(9).
Long KM, Ferris MT, Whitmore AC, Montgomery SA, Thurlow LR, McGee CE, Rodriguez CA, Lim JK, Heise MT. γδ T Cells Play a Protective Role in Chikungunya Virus-Induced Disease. Journal of virology 2015; 90(1).
Break TJ, Jaeger M, Solis NV, Filler SG, Rodriguez CA, Lim JK, Lee CC, Sobel JD, Netea MG, Lionakis MS. CX3CR1 is dispensable for control of mucosal Candida albicans infections in mice and humans. Infection and immunity 2015 Mar; 83(3).
Heaton NS, Langlois RA, Sachs D, Lim JK, Palese P, tenOever BR. Long-term survival of influenza virus infected club cells drives immunopathology. The Journal of experimental medicine 2014 Aug; 211(9).
Michlmayr D, Lim JK. Chemokine receptors as important regulators of pathogenesis during arboviral encephalitis. Frontiers in cellular neuroscience 2014; 8.
Lionakis MS, Swamydas M, Fischer BG, Plantinga TS, Johnson MD, Jaeger M, Green NM, Masedunskas A, Weigert R, Mikelis C, Wan W, Lee CC, Lim JK, Rivollier A, Yang JC, Laird GM, Wheeler RT, Alexander BD, Perfect JR, Gao JL, Kullberg BJ, Netea MG, Murphy PM. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival. The Journal of clinical investigation 2013 Dec; 123(12).
Bardina SV, Lim JK. The role of chemokines in the pathogenesis of neurotropic flaviviruses. Immunologic research 2012 Dec; 54(1-3).
Lionakis MS, Fischer BG, Lim JK, Swamydas M, Wan W, Richard Lee CC, Cohen JI, Scheinberg P , Gao JL, Murphy PM. Chemokine Receptor Ccr1 Drives Neutrophil-mediated Kidney Immunopathology and Mortality in Invasive Candidiasis. PLoS Path 2012 Aug; 8(8).
Wan W, Lim JK, Lionakis MS, Rivollier A, McDermott DH, Kelsall BL, Farber JM, Murphy PM. Genetic Deletion of Chemokine Receptor Ccr6 Decreases Atherogenesis in ApoE-Deficient Mice. Circ Res 2011 Jun;.
Lim JK, Murphy PM. Chemokine control of West Nile virus infection. Exp Cell Res 2011 Mar; 317(5): 569-74.
Lionakis MS, Lim JK, Lee CC, Murphy PM. Organ-Specific Innate Immune Responses in a Mouse Model of Invasive Candidiasis.. J Innate Immun 2010 Nov; 3(2): 180-99.
Lim JK, Obara CJ, Rivolier A, Pletnev AG, Murphy PM. Chemokine receptor Ccr2 is critical for monocyte accumulation and survival in West Nile virus encephalitis. J Immunol 2011 Jan; 186(1): 471-478.
Lim JK, McDermott DH, Lisco A, Foster GA, Krysztof D, Follmann D, Stramer SL, Murphy PM. CCR5 deficiency is a risk factor for early clinical manifestations of West Nile virus infection but not for viral transmission. The Journal of infectious diseases 2010 Jan; 201(2).
Lim JK, Lisco A, McDermott DH, Huynh L, Ward JM, Johnson B, Johnson H, Pape J, Foster GA, Kryztof D, Follmann D, Stramer SL, Margolis LB, Murphy PM. Genetic variation in OAS1 is a risk factor for initial infection with West Nile virus in man. PLoS Pathog 2009 Feb; 5(2:e1000321).
Lim JK, Louie CY, Glaser C, Jean C, Johnson B, Johnson H, McDermott DH, Murphy PM. Genetic deficiency of chemokine receptor CCR5 is a strong risk factor for symptomatic West Nile virus infection: a meta-analysis of 4 cohorts in the US epidemic. J Infect Dis 2008 Jan; 197(2): 262-265.
Lambert JS, Machado ES, Watson DC, Sill AM, Lim JK, Charurat M, Cunha SM, Afonso AO, Oliviera RH, Tanuri A, DeVico AL. Production of the HIV-suppressive chemokines CCL3/MIP-1alpha and CCL22/MDC is associated with more effective antiretroviral therapy in HIV-infected children. Pediatr Infect Dis J 2007 Oct; 26(10): 936-44.
Lim JK, Lu W, Hartley O, DeVico AL. N-terminal proteolytic processing by cathepsin G converts RANTES/CCL5 and related analogs into a truncated 4-68 variant. J Leukoc Biol 2006 Dec; 80(6): 1395-404.
Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD. Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.. Int Immunol 2006 Aug; 18(8): 1243-51.
Lim JK, Glass WG, McDermott DH, Murphy PM. CCR5: no longer a good-for-nothing gene--chemokine control of West Nile virus infection. Trends Immunol 2006 Jul; 27(7): 308-312.
Glass WG, McDermott DH, Lim JK, Lekhong S, Yu SF, Frank WA, Pape J, Cheshier RC, Murphy PM. CCR5 deficiency increases risk of symptomatic West Nile virus infection.. J Exp Med 2006 Jan; 203(1): 35-40.
Glass* WG, Lim* JK, Cholera R, Pletnev AG, Gao JL, Murphy PM. Chemokine receptor CCR5 promotes leukocyte trafficking to the brain and survival in West Nile virus infection.. J Exp Med 2005 Oct; 202(8): 1087-1098.
Lim JK, Burns JM, Lu W, DeVico AL. Multiple pathways of amino terminal processing produce two truncated variants of RANTES/CCL5. J Leukoc Biol 2005 Aug; 78(2): 442-52.
Berlyn KA, Ponniah S, Stass SA, Malone JG, Hamlin-Green G, Lim JK, Cottler-Fox M, Tricot G, Alexander RB, Mann DL, Malone RW. Developing dendritic cell polynucleotide vaccination for prostate cancer immunotherapy. Journal of biotechnology 1999 Aug; 73(2-3).
Lim JK, Hunter J, Fernandez-Vina M, Mann DL. Characterization of LMP polymorphism in homozygous typing cells and a random population.. Hum Immunol 1999 Feb; 60(2): 145-51.
Lim JK, Gunther NW 4th, Zhao H, Johnson DE, Keay SK, Mobley HL. In vivo phase variation of Escherichia coli type 1 fimbrial genes in women with urinary tract infection.. Infect Immun 1998 Jul; 66(7): 3303-10.