We at the Icahn School of Medicine at Mount Sinai (ISMMS) provide support for innovative cancer research through the following shared resources:
Cancer Cell Organoid Core
The Cancer Cell Organoid Core (CCOC) is an Oncological Sciences resource for the establishment of human tumor lines from ISMMS clinical specimens. New techniques in sample processing and cell culture methods have made it possible to reproducibly establish such lines as novel in vitro models of studying disease and modeling therapeutic strategies that may one day be applied clinically.
Presently, breast tumors of different subtypes and stages are being explanted into culture on an as available basis. Researchers can submit a short research proposal to the CCOC to obtain such cultures for their investigations. Culture reagents are the responsibility of the individual researchers, but protocols will be provided to assist researchers in maintenance of these cells.
Contact Pamela Cheung at firstname.lastname@example.org for additional information.
We maintain a state-of-the-art cryogenics facility for long-term storage of cell lines by ISMMS investigators. We can accommodate deposit and delivery of cells as well as viable freezing of cells on a regularly scheduled basis. You can rent a safety deposit box, which contains space for 81 individual vials for $60 per year, and you can negotiate discounts for larger needs. We can provide back-up to investigators who have their own freezers or serve as the primary means of viable cell storage. A cost estimate can be obtained by filling out a Cryogenic Facility Rental Request Form.
Contact Ed Hicks at email@example.com for additional information.
We provide tissue, embedding, sectioning and histochemistry services in support of ISMMS investigators involved in grant or institutionally funded cancer-related projects using a charge-back system for technologist time and effort. You can submit a request for limited support of non-funded pilot studies that are are aimed at developing preliminary data for cancer-relevant grant proposals. We can arrange for pathology consultation on an ad hoc basis. For a cost estimate, fill out a Histo-Immunology Lab Worksheet.
Contact Rui Qiao at firstname.lastname@example.org for additional information.
We offer fully automated immunohistochemistry (IHC), immunofluorescence staining (IF), and in situ hybridization (ISH) on cells and tissues. Our Immunostaining facility, managed by Mihaela Skobe, PhD, is equipped with a LeicaBond RX instrument (Leica Biosystems) that can stain up to 30 slides per run while testing multiple protocols simultaneously. The LeicaBond instrument offers the speed and flexibility required to optimize new antibodies or to process numerous slides/protocols in a timely manner with high-quality results. We can stain both frozen and FFPE tissues. We offer both validated protocols as well as direct assistance with protocol development.
For consultation and scheduling, contact Anita Rogic at email@example.com. You can reserve the LeicaBond RX online through eRAP.
Laser Capture Microscopy
We offer laser capture microscopy for micro-dissection of tissue sections. You can use this state-of-the-art equipment to identify and isolate fluorescent-labeled cells in tissue sections. We offer a free training session to allow investigators to use the equipment for a nominal charge ($25/hour). Our department technologists are also available to perform micro-dissection on a charge-back basis for time and effort. You can arrange for pathology consultation on an ad hoc basis.
Contact Rui Qiao at firstname.lastname@example.org for a cost estimate.
We offer a self-contained sequencing core that consists of the Nextseq 500 from Illumina and associated instruments for deep-sequencing including the Agilent 2100 bioanalyzer (for quality and quantity measurements of RNA and DNA), the Covaris M220 sonicator (for fragmenting RNA and DNA) and the Qubit fluorometer 2.0 (for nucleic acid quantification). These instruments offer fast turn-around time and the ability to perform real-time research and fine-tune methods in the lab. We have a single flow-cell that yields 400-500 million reads per sample, with a run time of approximately 8 hours (75 bp). We can do 75, 150 and 300 bp reads, paired-end or single-end. We can also do dual indices. This volume allows us to multiplex large numbers of samples (10 mRNAseq samples and many more for ChIPseq and other applications). We encourage users to share runs with others, to enable debugging, testing and reduce costs. We are trying to develop best practices and new methods, so please consult with us before proceeding with sample prep. We use BaseSpace to share data.
Contact Ravi Sachidanandam at email@example.com for additional information.