Flow Cytometry

Flow cytometry measures high-order multidimensional data with single cell granularity. This powerful science acquires these measurements at rates of thousands per second and can work with virtually unlimited population sizes, offering incredible statistical uses. To use this data and return a highly purified subpopulation of live functional cells is the magic of cell sorting.

Given this power, the CoRE provides all members of the Mount Sinai community the instrumentation and subject expertise to incorporate flow cytometry and cell sorting into any research project. Please explore these web pages to learn more about our amazing services and brilliant people.

Meet the Team, Flow Cytometry

Meet the scientists in charge of the Flow Cytometry CoRE.

Jordi Ochando, PhD
Director
jordi.ochando@mssm.edu
Dr. Ochando is Assistant Professor at the Department of Medicine and Oncological Sciences and is a member of the Immunology Institute and Tisch Cancer Institute. His laboratory investigates the origin, development and immune regulatory function of monocyte-derived suppressive cells (MDSC). Since MDSC have considerable relevance to the crucial problem as why a growing tumor cannot be rejected despite the recognition of tumor associated antigens, Dr. Ochando's  laboratory investigates the immunological mechanisms of tumor acceptance mediated by MDSC, which can be exploited to prevent allograft rejection in transplantation.

Christopher Bare
Laboratory Operations Manager
christopher.bare@mssm.edu
Christopher Bare has been a flow cytometry professional since 1995 in a variety of commercial and academic institutions including BD Biosciences, Rockefeller University, AnaptysBio, MSKCC, Weill Cornell, Galapagos Genomics, and UCSF Gladstone Institutes. His research experience has encompassed human disease from HIV to diabetes to psoriasis, as well as the prokaryotic and plant kingdoms. Chris received his degree from Cornell University, with additional graduate study at the University of Medicine and Dentistry of New Jersey.

XuQiang Qiao, PhD
Senior Associate Researcher
xuqiang.qiao@mssm.edu
Xuqiang Qiao has worked in the Mount Sinai flow facility since 2008, with his involvement if flow cytometry starting earlier in 2006. Before discovering his calling in cell sorting, he had worked 5 years in a bioresearch lab. Xuqiang Qiao received his bachelor degree from University of Electronic Science and Technology of China. 

Laura Grisanti
Senior Associate Researcher
Laura.grisanti@mssm.edu
Laura obtained her PhD degree in Cell Science and Cellular Technology from La Sapienza University of Rome, Italy. She joined Mount Sinai in January 2009 as a postdoc studying the function of niche stem cells during hair follicle morphogenesis and subsequently the epithelial regeneration of cornea. Laura joined the Flow Cytometry Facility in June 2016. 

Pei-Yu Kuo
Associate Scientist
Pei-yu.kuo@mssm.edu
Pei-Yu Kuo is an immunologist with 10+ years of experience in applying multi-color flow cytometry to basic science and translational fields such as oncological research and Immunology.   She has expertise in developing and optimizing protocols for enriching rare populations from human clinical samples and genetic-engineered mouse models using flow and magnetic sorting.  Her research interests have been focusing on hematological malignancies.  Pei-Yi is a certified clinical laboratory technologist. She received both her bachelor degree in Medical Technology and Master degree in Immunology from National Taiwan University.

Venu Pothula
Associate Scientist II
Venu.pothula@mssm.edu
Venu is our resident expert on nuclei sorting. He is deeply interested in developing new cytometry technology and is a co-inventor on hardware modifications to facilitate 384 well sorting for single cell sequencing applications. Venu joined the CORE in 2015 from the ISMMS Neuroscience Department.

About Acknowledgments

The lifeblood of an academic institution is publication. Likewise the metric of success of a shared resource facility is acknowledgement. Please remember to acknowledge the contribution of the CoRE to your publications.

Authorship, however, should follow the guidelines set forth in the Faculty Handbook.

The Flow Cytometry Center of Research Excellence maintains three rooms in the Icahn Medical Institute and two in the Hess Center for Science and Medicine within the Tisch Cancer Institute.

Room 13-05
This room contains the BD LSRII and FACSCantoII analyzer cytometers, as well as standalone data analysis workstations. This room is open access, however, cytometer use requires CoRE training. This room is also for user training.

Room 13-52a
This room contains two FACSAria cell sorters placed within biosafety cabinets to facilitate BSL2+ sorting of human and potentially pathogenic material. Access to this room is restricted to approved sorter users who have up-to-date bloodborne pathogen training. Access is through the CoRE Staff or by training as a self-operate sort user.

Room 13-72
This room contains the non-containment Influx six-way sorter. This room does not provide any biohazard containment and is restricted to non-human and non-pathogenic material. Access is through the CoRE Staff only.

Room S5-304
This room contains a FACSAria cell sorters placed within biosafety cabinet to facilitate BSL2+ sorting of human and potentially pathogenic material. Access to this room is restricted to approved sorter users who have up-to-date bloodborne pathogen training. Access is through the CoRE Staff or by training as a self-operate sort user.


Room S6-308
This room is an open access room containing data analysis workstations. This room also contains the Facility Manager and is a site for user training.