Equipment and Rates

The Flow Cytometry CoRE maintains all BD analyzers and cell sorters (with the exception of the Influx) with a detector configuration that allows for template portability, as well as preventing figures and data from being mislabeled in publication. The most common fluorochromes in each detector are listed in a table that can be used to choose the appropriate instrument for your samples.

Rates

All instrument usage rates are indicated at the point of reservation within the iLab.

Cancellation Charges

All scheduled sessions in the iLab system are subject to a 24 hour advance cancellation period. Cancellation within 24 hours of the scheduled start of a session will result in a charge equal to the entire scheduled session time. This cancellation charge will be pro-rated based upon assumption of the cancelled time by another user.

Data Workstations - No charge

The CoRE maintains PC workstations for use free-of-charge to analyze data generated within the CoRE. These workstations have up-to-date versions of DiVa, FlowJo, and Cytobank, as well as Microsoft Office. Other data analysis packages may be available as beta test versions predicated on Mount Sinai’s reputation for high volume flow cytometry and broad subject matter.

Two workstations are placed in IMI13-05 and two in CSM S6-308 for use on a 24/7 schedule.

Software

The CoRE provides a site license for FCS Express 5 Flow Cytometry analysis software. You may request a license and access additional resources to help you get started with FCS Express.

The team at De Novo Software is committed to your success. Contact support@denovosoftware.com for immediate assistance or register for free Q&A sessions.

Below are the various types of analyzers we employ.

Canto II

Manufactured by BD, this instrument features two lasers and six colors, as well as semi-automated fluidic operation.

LSRIIA

Manufactured by BD, this instrument features five lasers and 13 colors for maximum complexity of experimental design. This instrument has a high throughput HTS option available to read samples from 96 and 384 well microtitre plates.

LSRIIB

Manufactured by BD, this instrument features five lasers and 14 colors for maximum complexity of experimental design.

Our CoRE works with five different sorters as detailed below.

IMI3L

Manufactured by BD, this instrument features three lasers and ten colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation. 

IMI5L

Manufactured by BD, this instrument features five lasers and 14 colors inside a biosafety cabinet for sorting human or potentially infectious material.

CSM5L

Manufactured by BD, this instrument features five lasers and 16 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.

CSM4L

Manufactured by BD, this instrument features four lasers and 11 colors without biohazard containment, and therefore restricted to non-human and non-pathogenic material. This instrument is preferentially scheduled for self-operation.

Influx

Manufactured by BD, this instrument features five lasers and 13 colors without biohazard containment, and therefore restricted to non-human and non-pathogenic material. This instrument also features six-way sorting. The Influx optical configuration can be viewed here.