Mass Cytometer

The CyTOF2 is a state of the art mass cytometer that allows highly multi-parametric single cell analysis. Conceptually similar to conventional flow cytometry, mass cytometry employs heavy metal-conjugated antibodies instead of fluorescent antibodies to label populations of cells. This allows the simultaneous detection of 30-40 antibody tags with minimal signal overlap, minimal background and comparable signal intensity.

Before a CyTOF project can be initiated, all investigators must submit a CyTOF Project Proposal Form and schedule an initial meeting with Adeeb Rahman and Jordi Ochando.

The CORE offers training, consultation and assistance in all aspects of mass cytometry workflow including antibody panel design, antibody conjugation, sample preparation and data analysis. The CORE has compiled standardized panels of metal-labeled antibodies that can be used for in-depth profiling of major cell subsets in mice and humans. Custom antibody conjugations can be done upon request. Please contact Adeeb Rahman for more information on services and current antibody availability.


Initial consultation, panel development and project planning Fee
Additional consultation $50/hr
Antibody use from standard panels (DVS) $4/antibody/sample
Custom conjugated antibodies $8/antibody/sample
Sample acquisition on the CyTOF
(~200,000 events/10 minutes/0.5ml sample injection)
Data analysis/figure preparation $50/hr

National CyTOF Meeting

9:00-9:15 Steven Burakoff, MD, Director of The Tisch Cancer Institute; Lillian and Henry M. Stratton Professor of Cancer Medicine; Professor of Medicine, Hematology, and Medical Oncology; and Professor of Oncological Sciences, Icahn School of Medicine at Mount Sinai, Welcome Address

9:15-10:00 Erin Simonds, PhD, UCSF
Dissecting Intratumor Heterogeneity with CyTOF Mass Cytometry

10:15-10:40 Adeeb Rahman, PhD, Icahn School of Medicine at Mount Sinai
Strategies for Establishing an Effective Mass Cytometry Program

10:40-11:15 TJ Chen, PhD, Cytobank
Cytobank: Enabling Single Cell Analysis and Personalized Medicine

11:15-11:45 Josh Brody, MD, Assistant Professor Medicine, Hematology and Medical Oncology, Icahn School of Medicine at Mount Sinai
Turning a Tumor into a Vaccine Factory: In Situ Vaccination for Low-Grade

11:45-12:15 Michelle Poulin, PhD, Fluidigm Corp.
Considerations for Generating High Quality CyTOF Data

1:15-2:00 Brent Ferrell, MD, Vanderbilt University
Mass Cytometry Phenotyping of Early Therapy Response in Acute Myeloid

2:00-2:30 Scott Tanner, PhD, Fluidigm Corp.
The Evolution of Mass Cytometry Technologies

Wednesday, October 29, 2014
Davis Conference Center
Second Floor, Hess Center for Science and Medicine
Icahn School of Medicine at Mount Sinai
1470 Madison Ave
New York, NY 10029

Contact Us

Jordi Ochando, PhD
Tel: 212-824-8430 

Christopher Bare
Laboratory Operations Manager
Tel: 646-630-0142

Icahn Building IMI 13-05 Analyzers
Tel: 212-659-9440

Icahn Building IMI 13-52a Sorters 
Tel: 212-659-8201

Icahn Building IMI 13-72 Sorters
Tel: 212-659-9439

Hess Building CSM S5-304 Sorter
Tel: 212-824-9596

Hess Building CSM S6-204Sorter  
Tel: 212-824-9363

Hess Building CSM S6-308 Sorter
Tel: 212-824-9595


Hess Center for Science and Medicine
1470 Madison Avenue
5th Fl., Rm. S5-113
New York, New York 10029

Related Resources

CyTOF Project Proposal [PDF]

Cost Example [PDF]