Services

The main flow of work is separation of proteins of interest from their source material by liquid chromatography and identification of these proteins by mass spectrometric sequencing. The approach depends on the requirements of the customer and could be one or a combination of the following:

1. Development of a liquid chromatographic technique to separate an active protein(s) while retaining biological activity. Here active peaks will be identified by assays performed in the customer’s lab. The identity of protein(s) in active peaks will be determined by mass spectrometry. Proteomics Laboratory equipment used: Akta Explorer 100, BeckmanCoulter PF2D, ThermoFisher LTQ mass spectrometer (see “Equipment and Techniques” tab for description of this instrumentation).

2. Proteome Partitioning. Removal of the 12 most highly abundant protein species from serum and plasma using ProteomeLab IgY affinity chromatography yields an enriched pool of low abundance proteins for analysis by LC/MS/MS or for separation by chromatography or comparative proteomics. Proteomics Laboratory equipment used: Akta Explorer 100, BeckmanCoulter PF2D (see “Equipment and Techniques” tab for description of this instrumentation).

3. Comparative Chromatography in which comparable complex proteomes (such as treated and untreated cultivated cells) are separated by consecutive chromatographic procedures and the chromatographic profiles compared for differences in expression of proteins. Identity of proteins in difference peaks will be determined by mass spectrometry. Proteomics Laboratory equipment used: BeckmanCoulter PF2D (see “Equipment and Techniques” tab for description of this instrumentation).

4. Determination of the identity of proteins in a band or spot from 1D or 2D gels respectively or in a peak from liquid chromatography provided by the customer. Proteomics Laboratory equipment used: ThermoFisher LTQ mass spectrometer (see “Equipment and Techniques” tab for description of this instrument).

Note All preparation work prior to mass spectrometry (denaturation, alkylation and digestion) will be done by the Proteomics Laboratory in a protected environment to reduce the possibility for contamination.