FAQ

Learn how the Human Immune Monitoring CoRE can help your research, through our answers to the following frequently asked questions.

A:  We are highly experienced in measuring differences in cytokines, in the serum or other fluids, between cohorts as well as isolating RNA from samples for sequencing. The HIMC can compare serum antibody profiles to self-antigens in two populations. We can look at differences in regulatory T cells, myeloid-derived suppressor cells (MDSC), lymphocytes, etc. between cohorts by collecting peripheral mononuclear cells from blood or other sites.

A: We are highly experienced in measuring differences in cytokines, in the serum or other fluids, between cohorts as well as isolating RNA from samples for sequencing. The HIMC can compare serum antibody profiles to self-antigens in two populations. We can look at differences in regulatory T cells, myeloid-derived suppressor cells (MDSC), lymphocytes, etc. between cohorts by collecting peripheral mononuclear cells from blood or other sites via flow cytometry and/or CYTOF

A: The HIMC can perform flow cytometric and CyTO acquisition on samples that have been prepared and stained for in-depth analysis. Our equipment is meticulously calibrated and maintained to provide consistent readings. Our staff can also assist with the development of Standard Operating Procedures and quality control procedures.

A:  The HIMC can measure serum or plasma antibody responses specific for the antigen. We can also look for immune responses to unrelated antigens following immunization, explained by recognition of other targets related to the tumor or virus.

A: The HIMC can process and cryopreserve plasma, serum and peripheral blood mononuclear cells to ensure viability of cells for future studies studies via complete biospecimens life cycle management with bar-coding system though LIMS.

A: The HIMC can process and store blood for a variety of applications via complete biospecimens life cycle management with bar-coding system though LIMS. The HIMC can sort human cells, and works closely with the genomic CoRE to prepare samples for genomic analysis. Other biological fluids or tissue samples may be accepted. 

The HIMC performs Luminex assays, which allows for the measurement of panels of cytokines, chemokines, metabolic hormones, gut hormones, adipokines, stem cell markers, cell signaling molecules in cell extracts, and cardiovascular disease biomarkers in variety of sample matrix including serum, plasma, tissue/cell lysate and culture supernatants.