About Us

Director
Roger J. Hajjar, MD

Co-Directors
Yoshiaki Kawase, MD
Kiyotake Ishikawa, MD

Director of Operations
Kenneth Fish, PhD

Post-Doctoral Fellows
Jaume Aguero, MD

Technician
Lauren Leonardson, LVT

The newly built Cardiovascular Translational Laboratory of Physiology and Imaging is located on the 26th floor of the Annenberg building at the Mount Sinai Health System. It has a conventional cardiac catheterization laboratory for animal research that is identical to the human laboratories. It also has a surgical suite for animal cardiac surgery with cardiac bypass capabilities that are located in a single center. The dimension of this laboratory is 8.0 x 4.0 m. This catheter laboratory as shown below is equipped with a monoplane cardiovascular system for experiment procedures with a ceiling suspended C-arm stand and digital imaging. (INTEGRIS CV Systems, PHILIPS) A MARK V PROVIS INJECTOR (MEDRAD) is also installed for contrast injection. The laboratory serves as a pre-clinical testing site for gene and cell transfer. Large animal models with cardiovascular anatomy comparable to humans (ovine and porcine) are routinely studied in this laboratory.

Technologies Available in the Laboratory
All the available methods within the Large Animal Phenotyping and Gene Transfer Core are described below:

Table 1:

Device name Parameters to be measured
Trans-Thoracic Echocardiography (PHILIPS) 2D, 3D echo image. TDI, speckle strain analysis
High fidelity catheter tip micromanometry (Millar instrument) HR, Peak systolic left ventricular pressure, left ventricular end diastolic pressure, (dp/dt)max, (dp/dt)min, Tau (time constant of isovolumic relaxation) value measurement
Conductance catheter (Millar instrument) PV loop analysis
Swan-Ganz catheter Cardiac output, Right atrium pressure, Pulmonary artery pressure, Pulmonary vascular resistance, Systemic vascular resistance.
Coronary angiogram
(INTEGRIS CV Systems, PHILIPS)
High resolution angiogram. (100mm)
Aortogram with digital subtraction.
Mobile fluorescence imaging system Detection of the distribution of injected fluorescence dye and microspheres in vivo and in vitro setting. Using different wavelength fluorescence microspheres, the different distribution of microspheres with different modality or different timing within the same animal is possible.
Microplate readers and spectrophotometers (Synergy 2, BioTek) Precise regional coronary flow measurement using fluorescence microspheres is possible with this machine.
Reveal plus (Medtronic) Continuous monitor of arrhythmia is possible with this machine with a minimum invasive procedure. (3cm incision)

                  


Contact Us

Kenneth Fish, PhD
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Annenberg, 26th floor
Room 214