What’s New at the Molecular Pathology Lab
We frequently bring in new molecular tests with proven clinical utility for the diagnosis or monitoring of disease or for selecting targeted therapy. Here are recent updates to the clinical services that we offer:
BRAF GENOTYPING: BRAF is a serine/threonine-protein kinase, an important component in EGFR-RAS-RAF-MAPK pathway and frequently found to be mutated in various cancers. In 90% of cases, an activating point mutation results in the substitution of glutamic acid (Glu) for valine (VAL) at amino acid residue 600 (V600E). This mutation has been widely observed in papillary thyroid carcinoma (~50%), colorectal cancer (~5%), lung cancer (~2%), melanomas (80%), and hairy cell leukemia (~100%). BRAF genotyping has been shown to provide valuable clinical information for the treatment of colorectal carcinoma and melanoma.
- The Molecular Pathology lab offers BRAF mutation analysis using DNA extracted from the following specimens: 1) fresh tissue, 2) frozen (snap or OCT-embedded) tissue, and 3) formalin-fixed paraffin-embedded tissue block or unstained slides. Extracted DNA is analyzed by ARMS-PCR and gel electrophoresis. Histology slides (H&E) should also be submitted along with the specimens to evaluate specimen adequacy. The test is performed once a week, and can be ordered as a primary test (melanoma) or as a reflex test to wild-type KRAS genotyping (colorectal cancer).
- To order the BRAF genotyping assay, please contact the Molecular Pathology Laboratory (X89272) or fax the attached requisition to 212-427-2082.