The goal of the Translational Chemical Biology Center (TCBC) is to provide research investigators at Icahn School of Medicine access to small molecule chemical compounds that can be used as tools to advance discovery basic and translational research of human biology and disease, and also to facilitate validation and discovery of novel therapeutic targets for new disease treatment. Specifically, the Center is to provide sustained support of specialized technical expertise in organizing and maintaining various generic and focused chemical libraries, and in handling high-throughput screening (HTS) of small-molecule chemicals by using target-based biochemical assays as well as cell-based functional assays for research investigators at MSSM. The Center staff can assist investigators in design and development of various screening assays, and facilitate the exchange of assays being developed among participating laboratories and also organize training activities. The main functions of the Small-Molecule Screening Facility in the TCBC are to:
- Format and maintain libraries of small-molecule chemical compounds for HTS;
- Assist researchers in creating and formatting high-throughput assays;
- Perform target structure-based chemical screening;
- Assist with selection of compounds to be purchased and tracking and analyzing screening data;
- Facilitate structure-activity relationship (SAR) analyses following chemical screening by structure-based, biochemical or in silico methods;
- Assist in design of new compounds to be synthesized, and coordinate purchase of compound libraries.
The chemical screening resources in the TCBC include libraries of chemical compounds (i.e. a collection of 115,000 chemicals, of which a library of 14,000 compounds are formatted in DMSO-d6 for NMR structure-based screening) and automatic liquid handling robotic systems (TECAN EVO 150 and 200). In addition, the 500 MHz NMR spectrometer in the Mount Sinai's NMR Facility that is equipped with an automated NMR sample changer is available for NMR-based chemical screening. It is expected that an expanded collection of protein family-based chemical libraries will be generated through combinatorial or parallel synthesis. The handling of these chemical libraries (including chemical analysis and library formatting) and "customization" for individual screening will be carried out by staff chemists at the Center.