Icahn School of Medicine at Mount Sinai
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Resources Genomics Core Facility Genomic Technology Applications

Genomic Technology Applications

Next-generation sequencing using the Illumina and Pacific Biosciences platforms enables genome-wide view of biology. This section highlights methods currently available as a service from the genomics facility.


Genetic Event/Application Method Available Technology
Single molecule sequencing Amplification free library preparation PacBio RS
5-Methylcytosine Methylation arrays
Bisulfite sequencing
Illumina BeadArray
Illumina HiSeq 2500
PacBio RS
Discovery of novel epigenetic modifications Direct observation of polymerase kinetics PacBio RS
Whole-genome sequencing for organisms with no genome assembly (bacteria, viruses, non-model organisms) Amplification-free 2kb library preparation Illumina gDNA library preparation PacBio RS Illumina HiSeq 2500
Somatic and germ line mutations, deletions, genomic rearrangements and CNVs Genotyping by microarray Whole-Exome sequencing Amplicon sequencing Illumina BeadArray
Illumina HiSeq 2500
PacBio RS
Chromatin structure and in-vivo protein/DNA interactions Chromatin Immuno-precipitation and library preparation ("ChIP-Seq") Illumina HiSeq 2500
Illumina MiSeq
Plasmid/Amplicon Sequencing Sanger dye-terminator sequencing AB 3730xl Capillary Sequener
Gene expression cDNA library construction ("RNA-Seq")
cRNA direct Hybridization
Illumina HiSeq 2500
PacBio RS
Alternative splicing cDNA library construction followed by junction analysis Illumina HiSeq 2500
PacBio RS
Small RNA profiling and discovery RNA adapter ligation and PCR Illumina MiSeq
Illumina HiSeq2500


Gene Transcript Profiling Whole Exome Sequencing