s Technology Platforms - Icahn Institute - Genomics and Multiscale Biology
Icahn School of Medicine at Mount Sinai
Mount Sinai - Twitter Mount Sinai - YouTube Mount Sinai - Facebook

About

Resources Genomics Core Facility Technology Platforms

Technology Platforms

DNA Sequencing and nucleic acid characterization instruments

  • Illumina HiSeq 2000 - second generation sequencer from Illumina; capacity of 120-150M reads of 100 nt in length. Libraries may be sequenced from one or both ends
  • Illumina MiSeq - "personal" second generation sequencer based on HiSeq chemistry; capacity of 12-15M reads in 24 hours
  • Pacific Biosciences RS - third generation single molecule sequencer with read lengths up to 6kb
  • Applied Biosystems 3730xl - 96 capillary dye-terminator sequencer
  • Applied Biosystems 7900HT - real-time thermocycler for quantitative PCR and end-point allele discrimination for use by GGS department members

Microarray-based SNP and Transcriptomics Instruments

  • Illumina HiScan - most adavanced bead-array reader on the market for high-density SNP detection, mRNA-profiling and methylation microarrays
  • Illumina BeadXPress - medium density, custom SNP detection platform

Sample Preparation Instruments

  • Covaris E210 Focused Acoustic Disrupter - sonicator for automated high yield preparation of multiple genomic/mRNA libraries
  • Two TECAN Liquid Handling robot - Automated Bead Array hybridization and washing
  • Three Agilent BioAnalyzer - microfluidics-based platform for sizing, quantification and quality control of DNA and RNA; crucial in preparation of libraries for Illumina sequencing
  • Agilent Bravo - sample preparation of Illumina sequencing libraries using SPRI magnetic bead technology
  • Beckman BioMek FX liquid handling robot - automated Sanger sequencing reaction cleanup and real-time PCR plate set-up