Jianlong Wang, PhD

• 2013
  The Dr. Harold and Golden Lamport Research Award
  

• 2013
  Irma T. Hirschl and Weill-Caulier Trusts Career Scientist Award
  

• 2007
  Cell Development Award
  

• 2000
  LRFA Postdoctoral Fellowship
  

• 1995
  University Graduate Fellowship
  

• 1988
  University Undergraduate Award
  

Specific Clinical/Research Interest:
Genetic and epigenetic regulation of stem cell pluripotency and somatic cell reprogramming

Wang Lab Personnel:
Current Members
Xin Huang, Ph.D.
Title: Postdoctoral Fellow
Nationality: Chinese
Research Interest: Proteomics, Epigenetics
Email: xin.huang@mssm.edu

Diana Guallar, Ph.D.
Title: Postdoctoral Fellow
Nationality: Spanish
Research Interest: Transcriptional control of stem cell pluripotency
Email: diana.guallar@mssm.edu

Miguel Fidalgo, Ph.D.
Title: Postdoctoral Fellow
Nationality: Spanish
Research Interest: Naive and primed pluripotency and somatic cell reprogramming
Email: miguel.fidalgo-perez@mssm.edu

Francesco Faiola, Ph.D.
Title: Postdoctoral Fellow
Nationality: Italian
Research Interest: Protein-Protein Interaction and Stem Cell Pluripotency
Email: francesco.faiola@mssm.edu

Junjun Ding, Ph.D.
Title: Postdoctoral Fellow
Nationality: China
Research Interest: Epigenetic regulation of stem cell pluripotency
Email: junjun.ding@mssm.edu

Arven Saunders, M.S.
Title: PhD candidate
Nationality: USA
Research Interest: Nanog control of pluripotency and reprogramming

Former members:
Chandra P. Shekar, Ph.D.
Title: Postdoctoral Fellow (2009-2010)
Nationality: India
Research Interest: Novel pluripotency factors for stem cell pluripotency and development
Email: prabhc01@mssm.edu

Sarah H. Orkin, B.S. (2009-2010)
Title: Research Coordinator
Nationality: United States
Research Interest: Technician and lab manager

Summary of Research Studies:
Embryonic stem (ES) cells serve as a potentially inexhaustible source for tissue replacement in regenerative medicine due to their capability of unlimited self-renewal and multi-lineage differentiation. Vital cellular functions of ES cells require the coordinated action of a large number of proteins that assemble into an array of multi-protein complexes of distinct composition and structure (protein-protein interactions). In addition, physical interactions between regulatory pluripotency transcription factors and their target genes (protein-DNA interactions) provide insights into differential gene expression dictating the pluripotency program. Analysis of protein complexes encompassing intricate protein-protein and regulatory protein-DNA interactions is key to understanding stem cell pluripotency.

Recently, we tested the utility of in vivo biotinylation of transcription factors in mouse ES cells, and have established an in vivo biotinylation system for BirA-mediated specific biotinylation of critical pluripotency factors in mouse ES cells. We developed and optimized an approach for affinity purification of pluripotency protein complexes involving streptavidin capture of biotinylated proteins (dubbed bioSAIP) and demonstrated the feasibility of in vivo biotinylation for mapping global/chromosomal targets of many different transcription factors (dubbed bioChIP-chip). Utilizing the technologies we developed, we have constructed a protein interaction network surrounding the pluripotency factor Nanog in mouse ES cells (Wang et al., Nature 2006) and mapped an extended transcriptional network for pluripotency of mouse ES cells (Kim et al. Cell 2008). The network is highly enriched for factors known to be critical in ES cell biology and appears to function as a module for pluripotency. Pluripotency is maintained by many transcription factors that form a highly interconnected protein interaction network including the two homeobox proteins Nanog and Oct4, and a battery of associated proteins of known and unknown functions linking to multiple co-repressor pathways.

Further dissection of the pluripotency network in human ES cells (and induced pluripotent stem cells) and understanding molecular function of the novel factors should illuminate fundamental properties of stem cells and the process of cellular reprogramming, and ultimately lead to precise manipulation and realization of the full clinical therapeutic benefits of these unique cells. Therefore, my lab will be focusing on the following three research areas:
1) Defining protein-protein and protein-DNA interaction networks for pluripotency of human ES cells (and human iPS cells);
2) Dissecting molecular action of novel pluripotency factors on stem cell self-renewal and pluripotency;
3) Elucidating functional significance of novel pluripotency factors in early development and somatic cell reprogramming.

For more information, please visit the Wang Laboratory website.

Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature 2006; 444: 364-368.

Ackerman KG, Wang J, Luo L, Fujiwara Y, Orkin SH, Beier DR. Gata4 is necessary for normal pulmonary lobar development. Am J Respir Cell Mol Biol 2007; 36: 391-397.

Wang J, Theunissen TW, Orkin SH. Site-directed, virus-free and inducible RNAi in embryonic stem cells. Proc. Natl. Acad. Sci. USA 2007; 104: 20850-20855.

Wang J, Orkin SH. A protein roadmap to pluripotency and faithful reprogramming. Cells Tissues Organs 2008; 188(1-2): 23-30.

Levasseur DN, Wang J, Dorschner MO, Stamatoyannopoulos JA, Orkin SH. Oct4 dependence of chromatin structure within the extended Nanog locus in ES cells. Genes and Development 2008; 22(5): 575-580.

Kim J, Chu J, Shen X, Wang J, Orkin SH. An extended transcriptional network for pluripotency of embryonic stem cells. Cell 2008; 132(6): 1049-1061.

Wang J, Levasseur DN, Orkin SH. Requirement of Nanog dimerization for stem cell self-renewal and pluripotency. Proc. Natl. Acad. Sci. USA 2008; 105(17): 6326-6331.

Wang J, Trowbridge JJ, Rao S, Orkin SH. Proteomic studies of stem cells. (edited by Bernstein, B. E. and Lemischka, I.), published online in stembook.org. Stembook 2008;(1.4.1).

Orkin SH, Wang J, Kim J, Chu J, Rao S, Theunissen TW, Shen X, Levasseur DN. The transcriptional network controlling pluripotency in ES cells. Cold Spring Harbor Symposia on Quantitative Biology 2008;(73): 195-202.

Kim J, Cantor AB, Orkin SH, Wang J. Use of in vivo biotinylation both protein-protein and protein-DNA interaction networks in mouse embryonic stem cells. Nature Protocols 2009;(4): 506-517.

Wang J, Cantor AB, Orkin SH. Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation. Current Protocol in Stem Cell Biology 2009;(Chapter 1): Unit 1B.5-1B.5.17.

Wang J. Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs. Methods Mol Biol. 2010;(650): 241-256.

Fidalgo M, Shekar PC, Ang YS, Fujiwara Y, Orkin SH, Wang J. Zfp281 functions as a transcriptional repressor for pluripotency of mouse embryonic stem cells. Stem cells (Dayton, Ohio) 2011 Nov; 29(11).

Ang YS, Tsai SY, Lee DF, Monk J, Su J, Ratnakumar K, Ding J, Ge Y, Darr H, Chang B, Wang J, Rendl M, Bernstein E, Schaniel C, Lemischka IR. Wdr5 mediates self-renewal and reprogramming via the embryonic stem cell core transcriptional network. Cell 2011 Apr; 145(2).

Ding J, Xu H, Faiola F, Ma'ayan A, Wang J. Oct4 links multiple epigenetic pathways to the pluripotency network. Cell research 2012 Jan; 22(1).

Fidalgo M, Faiola F, Pereira CF, Ding J, Saunders A, Gingold J, Schaniel C, Lemischka IR, Silva JC, Wang J. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proceedings of the National Academy of Sciences of the United States of America 2012 Oct; 109(40).

MacArthur BD, Sevilla A, Lenz M, Müller FJ, Schuldt BM, Schuppert AA, Ridden SJ, Stumpf PS, Fidalgo M, Ma'ayan A, Wang J, Lemischka IR. Nanog-dependent feedback loops regulate murine embryonic stem cell heterogeneity. Nature cell biology 2012 Nov; 14(11).

Saunders A, Faiola F, Wang J. Concise review: pursuing self-renewal and pluripotency with the stem cell factor nanog. Stem cells (Dayton, Ohio) 2013 Jul; 31(7).

Costa Y, Ding J, Theunissen TW, Faiola F, Hore TA, Shliaha PV, Fidalgo M, Saunders A, Lawrence M, Dietmann S, Das S, Levasseur DN, Li Z, Xu M, Reik W, Silva JC, Wang J. NANOG-dependent function of TET1 and TET2 in establishment of pluripotency. Nature 2013 Mar; 495(7441).

Huang X, Wang J. A Determined . Molecular cell 2017 Apr; 66(2).

Yang J, Guo R, Wang H, Ye X, Zhou Z, Dan J, Wang H, Gong P, Deng W, Yin Y, Mao S, Wang L, Ding J, Li J, Keefe DL, Dawlaty MM, Wang J, Xu G, Liu L. Tet Enzymes Regulate Telomere Maintenance and Chromosomal Stability of Mouse ESCs. Cell reports 2016 May;.

Guallar D, Wang J. Taking the RISC of exiting naïve pluripotency. Genome biology 2016 May; 17(1).

Fidalgo M, Huang X, Guallar D, Sanchez-Priego C, Valdes VJ, Saunders A, Ding J, Wu WS, Clavel C, Wang J. Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States. Cell stem cell 2016 Jun;.

Zhang W, Shi S, Wang Y, Yu S, Zhu W, Zhang X, Zhang D, Yang B, Wang X, Wang J. Versatile molybdenum disulfide based antibacterial composites for in vitro enhanced sterilization and in vivo focal infection therapy. Nanoscale 2016 Jun; 8(22).

Chen H, Sun Z, Yang Z, Zhang Z, Wang J, Feng M, Yang Q. Degradation of 3,4-dichlorobenzotrifluoride by the Fenton-like process using zirconia-coated magnetite magnetic nanoparticles as an effective heterogeneous catalyst. Environmental science and pollution research international 2017 Aug; 24(22).

Song L, Chen L, Wang J, Chen F, Lan G. Prediction of crystal morphology of 3,4-Dinitro-1H-pyrazole (DNP) in different solvents. Journal of molecular graphics & modelling 2017 Aug; 75.

Faiola F, Yin N, Fidalgo M, Huang X, Saunders A, Ding J, Guallar D, Dang B, Wang J. NAC1 Regulates Somatic Cell Reprogramming by Controlling Zeb1 and E-cadherin Expression. Stem cell reports 2017 Sep; 9(3).

Wang S, Wang J. Trimethoprim degradation by Fenton and Fe(II)-activated persulfate processes. Chemosphere 2017 Oct; 191.

Liu Y, Zhou A, Liu Y, Wang J. Enhanced degradation and mineralization of 4-chloro-3-methyl phenol by Zn-CNTs/O3 system. Chemosphere 2017 Oct; 191.

Wang S, Wang J. Biodegradation and metabolic pathway of sulfamethoxazole by a novel strain Acinetobacter sp. Applied microbiology and biotechnology 2017 Oct;.

Wang J, Nguyen AV. A review on data and predictions of water dielectric spectra for calculations of van der Waals surface forces. Advances in colloid and interface science 2017 Oct;.

Yin Y, Zhang Y, Karakashev DB, Wang J, Angelidaki I. Biological caproate production by Clostridium kluyveri from ethanol and acetate as carbon sources. Bioresource technology 2017 Oct; 241.

Yang G, Wang J. Co-fermentation of sewage sludge with ryegrass for enhancing hydrogen production: Performance evaluation and kinetic analysis. Bioresource technology 2017 Nov; 243.

Huang X, Balmer S, Yang F, Fidalgo M, Li D, Guallar D, Hadjantonakis AK, Wang J. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. eLife 2017 Nov; 6.

Yang Y, Chen J, Huang Q, Tang S, Wang J, Hu P, Shao G. Can liming reduce cadmium (Cd) accumulation in rice (Oryza sativa) in slightly acidic soils? A contradictory dynamic equilibrium between Cd uptake capacity of roots and Cd immobilisation in soils. Chemosphere 2017 Nov; 193.

Huang L, Zhu W, Zhang W, Chen K, Wang J, Wang R, Yang Q, Hu N, Suo Y, Wang J. Layered vanadium(IV) disulfide nanosheets as a peroxidase-like nanozyme for colorimetric detection of glucose. Mikrochimica acta 2017 Dec; 185(1).

Tao Y, Xin M, Cheng H, Huang Z, Hu T, Zhang T, Wang J. TRIM37 promotes tumor cell proliferation and drug resistance in pediatric osteosarcoma. Oncology letters 2017 Dec; 14(6).

Luo S, Wang J. MOF/graphene oxide composite as an efficient adsorbent for the removal of organic dyes from aqueous solution. Environmental science and pollution research international 2017 Dec;.

Zhang Y, Xiang Y, Yin Q, Du Z, Peng X, Wang Q, Fidalgo M, Xia W, Li Y, Zhao ZA, Zhang W, Ma J, Xu F, Wang J, Li L, Xie W. Dynamic epigenomic landscapes during early lineage specification in mouse embryos. Nature genetics 2017 Dec;.

Ma M, Zhe T, Ma Y, Wang Z, Chen Q, Wang J. Highly sensitive and reproducible non-enzymatic glucose sensor fabricated by drop-casting novel nanocomposite with 3D architecture and tailorable properties prepared in controllable way. Talanta 2017 Dec; 180.

Yang G, Wang J. Pretreatment of grass waste using combined ionizing radiation-acid treatment for enhancing fermentative hydrogen production. Bioresource technology 2018 Jan; 255.

Yang G, Wang J. Kinetics and microbial community analysis for hydrogen production using raw grass inoculated with different pretreated mixed culture. Bioresource technology 2018 Jan; 247.

Wang S, Yin Y, Wang J. Microbial degradation of triclosan by a novel strain of Dyella sp. Applied microbiology and biotechnology 2018 Feb; 102(4).

Liu L, Chen S, Xue Z, Zhang Z, Qiao X, Nie Z, Han D, Wang J, Wang T. Bacterial capture efficiency in fluid bloodstream improved by bendable nanowires. Nature communications 2018 Feb; 9(1).

Yang Y, Chen J, Huang Q, Tang S, Wang J, Hu P, Shao G. Can liming reduce cadmium (Cd) accumulation in rice (Oryza sativa) in slightly acidic soils? A contradictory dynamic equilibrium between Cd uptake capacity of roots and Cd immobilisation in soils. Chemosphere 2018 Feb; 193.

Wang J, Yin Y. Fermentative hydrogen production using pretreated microalgal biomass as feedstock. Microbial cell factories 2018 Feb; 17(1).

Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Nature genetics 2018 Mar; 50(3).

Liu X, Zhang W, Huang L, Hu N, Liu W, Liu Y, Li S, Yang C, Suo Y, Wang J. Fluorometric determination of dopamine by using molybdenum disulfide quantum dots. Mikrochimica acta 2018 Mar; 185(4).

Miao J, Wang W, Wu S, Zang X, Li Y, Wang J, Zhan R, Gao M, Hu M, Li J, Chen S. miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018 Mar; 45(5).

Yue Z, Zhu W, Li Y, Wei Z, Hu N, Suo Y, Wang J. Surface Engineering of a Nickel Oxide-Nickel Hybrid Nanoarray as a Versatile Catalyst for Both Superior Water and Urea Oxidation. Inorganic chemistry 2018 Apr; 57(8).

Sun Y, Li K, Zhao J, Wang J, Tang N, Zhang D, Guan T, Jin Z. Nitrogen and sulfur Co-doped microporous activated carbon macro-spheres for CO capture. Journal of colloid and interface science 2018 Apr; 526.

Wang J, Wang S. Microbial degradation of sulfamethoxazole in the environment. Applied microbiology and biotechnology 2018 Apr; 102(8).

Wang J, Wu X, Wei C, Huang X, Ma Q, Huang X, Faiola F, Guallar D, Fidalgo M, Huang T, Peng D, Chen L, Yu H, Li X, Sun J, Liu X, Cai X, Chen X, Wang L, Ren J, Wang J, Ding J. YY1 Positively Regulates Transcription by Targeting Promoters and Super-Enhancers through the BAF Complex in Embryonic Stem Cells. Stem cell reports 2018 Apr; 10(4).

Chen K, Zhang W, Pan X, Huang L, Wang J, Yang Q, Hu N, Suo Y, Zhang D, Wang J. Natural Sugar: A Green Assistance To Efficiently Exfoliate Inorganic Layered Nanomaterials. Inorganic chemistry 2018 May; 57(9).

Yang G, Wang J. Pretreatment of grass waste using combined ionizing radiation-acid treatment for enhancing fermentative hydrogen production. Bioresource technology 2018 May; 255.

Tang J, Wang J. Metal Organic Framework with Coordinatively Unsaturated Sites as Efficient Fenton-like Catalyst for Enhanced Degradation of Sulfamethazine. Environmental science & technology 2018 May; 52(9).

Dou L, Zhao B, Bu T, Zhang W, Huang Q, Yan L, Huang L, Wang Y, Wang J, Zhang D. Highly sensitive detection of a small molecule by a paired labels recognition system based lateral flow assay. Analytical and bioanalytical chemistry 2018 May; 410(13).

Luo J, Zhou H, Rao L, Liu T, Liu L, Chen Y, Wang J, Hu T, Liu S, Chen D, Wang H, Zeng L, Huang J. A New Method of Biostorage and Biopreservation for Human Amputated Extremities. Biopreservation and biobanking 2018 Jun;.

Wei Z, Zhu W, Li Y, Ma Y, Wang J, Hu N, Suo Y, Wang J. Conductive Leaflike Cobalt Metal-Organic Framework Nanoarray on Carbon Cloth as a Flexible and Versatile Anode toward Both Electrocatalytic Glucose and Water Oxidation. Inorganic chemistry 2018 Jul; 57(14).

Han S, Tan C, Ding J, Wang J, Ma'ayan A, Gouon-Evans V. Endothelial cells instruct liver specification of embryonic stem cell-derived endoderm through endothelial VEGFR2 signaling and endoderm epigenetic modifications. Stem cell research 2018 Jul; 30.

Yang Q, Wang Y, Wang J, Liu F, Hu N, Pei H, Yang W, Li Z, Suo Y, Wang J. High effective adsorption/removal of illegal food dyes from contaminated aqueous solution by Zr-MOFs (UiO-67). Food chemistry 2018 Jul; 254.

Wang S, Hu Y, Wang J. Biodegradation of typical pharmaceutical compounds by a novel strain Acinetobacter sp. Journal of environmental management 2018 Jul; 217.

Wang J, Yang Q, Zhang L, Liu M, Hu N, Zhang W, Zhu W, Wang R, Suo Y, Wang J. A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples. Food chemistry 2018 Aug; 257.

Yue X, Liu L, Li Z, Yang Q, Zhu W, Zhang W, Wang J. Highly specific and sensitive determination of propyl gallate in food by a novel fluorescence sensor. Food chemistry 2018 Aug; 256.

Cheng R, Kang M, Zhuang S, Wang S, Zheng X, Pan X, Shi L, Wang J. Removal of bacteriophage f2 in water by Fe/Ni nanoparticles: Optimization of Fe/Ni ratio and influencing factors. The Science of the total environment 2018 Aug; 649.

Pei H, Wang J, Yang Q, Yang W, Hu N, Suo Y, Zhang D, Li Z, Wang J. Interfacial growth of nitrogen-doped carbon with multi-functional groups on the MoS skeleton for efficient Pb(II) removal. The Science of the total environment 2018 Aug; 631-632.

Wan Z, Wang J. Fenton oxidation of municipal secondary effluent: comparison of Fe/Ce-RGO (reduced graphene oxide) and Fe as catalysts. Environmental science and pollution research international 2018 Sep;.

Yan L, Dou L, Bu T, Huang Q, Wang R, Yang Q, Huang L, Wang J, Zhang D. Highly sensitive furazolidone monitoring in milk by a signal amplified lateral flow assay based on magnetite nanoparticles labeled dual-probe. Food chemistry 2018 Sep; 261.

Huang Q, Bu T, Zhang W, Yan L, Zhang M, Yang Q, Huang L, Yang B, Hu N, Suo Y, Wang J, Zhang D. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier. Food chemistry 2018 Oct; 262.

Zhuang S, Zhu X, Wang J. Kinetic, equilibrium, and thermodynamic performance of sulfonamides adsorption onto graphene. Environmental science and pollution research international 2018 Oct;.

Yang G, Wang J. Improving mechanisms of biohydrogen production from grass using zero-valent iron nanoparticles. Bioresource technology 2018 Oct; 266.

Wang W, Zhan R, Zhou J, Wang J, Chen S. MiR-10 targets NgR to modulate the proliferation of microglial cells and the secretion of inflammatory cytokines. Experimental and molecular pathology 2018 Oct;.

Peng M, Nguyen AV, Wang J, Miller R. A critical review of the model fitting quality and parameter stability of equilibrium adsorption models. Advances in colloid and interface science 2018 Oct;.

Wu S, Liu W, Ma Q, Yu W, Guo Y, Zhao Y, Shi D, Liu Y, Zhou Z, Wang J, Liu R, Zhou Y. Association Between Insulin Resistance and Coronary Plaque Vulnerability in Patients With Acute Coronary Syndromes: Insights From Optical Coherence Tomography. Angiology 2018 Nov;.

Yin Y, Wang J. Pretreatment of macroalgal Laminaria japonica by combined microwave-acid method for biohydrogen production. Bioresource technology 2018 Nov; 268.

Wang J, Zhuan R, Chu L. The occurrence, distribution and degradation of antibiotics by ionizing radiation: An overview. The Science of the total environment 2019 Jan; 646.

Liu M, Wang J, Yang Q, Hu N, Zhang W, Zhu W, Wang R, Suo Y, Wang J. Patulin removal from apple juice using a novel cysteine-functionalized metal-organic framework adsorbent. Food chemistry 2019 Jan; 270.

Bu T, Huang Q, Yan L, Zhang W, Dou L, Huang L, Yang Q, Zhao B, Yang B, Li T, Wang J, Zhang D. Applicability of biological dye tracer in strip biosensor for ultrasensitive detection of pathogenic bacteria. Food chemistry 2019 Feb; 274.