Protocols

Mouse Embryonic Fibroblast Generation and Expansion

Generation of p0 Swiss-Webster Mouse Embryonic Fibroblasts (MEFs) [PDF]
Thawing Mouse Embryonic Fibroblasts (MEFs) [PDF]
Mouse Embryonic Fibroblast (MEF) Expansion/Freezing [PDF]

Human Embryonic Stem Cell Protocols

Human Embryonic Stem Cell (hESC) Media Constituents [PDF]
Thawing Human Embryonic Stem Cells (hESCs) [PDF]
Passaging Human Embryonic Stem Cells (hESCs) [PDF]
Preparing Matrigel Plates [PDF]
MEF Depletion/Splitting hESCs onto Matrigel [PDF]
Human Embryonic Stem Cell (hESC) Freezing Protocol [PDF]
Alkaline Phosphatase Staining [PDF]
Karyotyping Protocol [PDF]

Mouse Embryonic Stem Cell Protocols

Thawing Feeders and Mouse Embryonic Stem Cells (mESCs) [DOC]
Passage 1x onto Gelatin or Feeders using DMEM-ES Media (1-2 days after thawing mESCs) [DOC]
Freezing mESCs [DOC]
Serum-Free mESC Differentiation [DOC]
Serum Based Differentiation Media Constituents [DOC]