Widefield Microscopy

This automated upright widefield microscope from ZEISS is the right choice for imaging standard glass slides containing fixed- or live-cell samples. Color and monochrome cameras are available for Brightfield/Phase/DIC and fluorescence imaging, respectively. A ten-position filter turret gives the Imager.Z2(M) a large flexibility in the florophores it can image without the need to stop and swap filter sets.

ZEISS' ZEN blue software package controls the microscope and offers multi-color imaging in conjunction with tile scanning, Z-stacking and time series acquisition. Advanced tiling functionality allows users to easily incorporate a focus map that accurately follows the changing plane of sectioned tissue; capturing Z-stacks in order to account for focal disparities is no longer necessary, and file sizes on large tile images is greatly reduced.

The ApoTome.2 optical sectioning device offers confocal-like image quality through the use of structured illumination. Compatible with all lenses and filter sets, ApoTome.2 improves fluorescence image quality by filtering out out-of-focus light to reveal detail in just the focal planes of interest.

Zeiss AxioImager.Z2(M) for fixed- or live-cell samples.

Zeiss AxioImager.Z2(M) for fixed- or live-cell samples

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

AT350/50x

T400LP

ET460/50m

GFP/FITC

ET470/40x

T495LP

ET525/50m

TRITC

ET545/40x

T570LP

ET620/60m

Cy5

ET620/60x

T660LPXR

ET700/75m

Cy7

ET710/75x

T760LPXR

ET810/90m

CFP*

ET436/20x

T455LP

ET480/40m

YFP*

ET500/20x

T515LP

ET535/30m

* Available on request

Objectives

Magnification

Immersion

N.A.

10X

air

0.3

20X

air

0.8

40X

air

0.75

40X

oil

1.3

63X

oil

1.4

100X

oil

1.4

Brightfield Protocol
Fluorescence Protocol 

This automated upright widefield microscope from ZEISS is the right choice for imaging standard glass slides containing fixed- or live-cell samples. Color and monochrome cameras are available for Brightfield/Phase/DIC and fluorescence imaging, respectively. A ten-position filter turret gives the Imager.Z2(M) a large flexibility in the florophores it can image without the need to stop and swap filter sets.

ZEISS' ZEN blue software package controls the microscope and offers multi-color imaging in conjunction with basic tile scanning, Z-stacking and time series acquisition.

The ApoTome.2 optical sectioning device offers confocal-like image quality through the use of structured illumination. Compatible with all lenses and filter sets, ApoTome.2 improves fluorescence image quality by filtering out out-of-focus light to reveal detail in just the focal planes of interest.

Zeiss AxioImager.Z2 for fixed- or live-cell samples

Zeiss AxioImager.Z2 for fixed- or live-cell samples

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

AT350/50x

T400LP

ET460/50m

GFP

ET470/40x

T495LPXR

ET525/50m

Texas Red

ET560/40x

T585LPXR

ET630/75m

Cy5

ET620/60x

T660LPXR

ET700/75m

CFP

ET436/20x

T455LP

ET480/40m

YFP

ET500/20x

T515LP

ET535/30m

* Available on request

Objectives

Magnification

Immersion

N.A.

2.5X*

air

0.075

5X*

air

0.15

10X

air

0.75

20X

air

0.75

40X

oil

1.3

63X

oil

1.4

100X

oil

1.4

Brightfield Protocol
Fluorescence Protocol

This flagship inverted microscope from Leica features a motorized XY stage for tiled fields and Navigator software module for automated tile acquisition; a TokaiHit stage-top incubation system and time lapse module for long-term live-cell imaging; and a SpectraX four-LED illumination system capable of fast, multi-color widefield fluorescence imaging. The system is equipped with both color and monochrome cameras for Brightfield/Phase/DIC and fluorescence imaging, respectively. Operated via Leica's LAS X software suite, the DMi8 easily images live- and fixed-cell samples in Petri dishes, well plates, chamber slides and standard glass slides. Fast widefield FRET imaging (CFP/YFP) can also be configured. Injection of drugs is possible via openings in the stage-top incubation system.

Microscope

Leica DMi8

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

BP 405/60

455

BP 470/40

GFP

BP 470/40

495

BP 525/50

Texas Red

BP 560/40

585

BP 630/75

Cy5

BP 620/60

660

BP 700/75


Objectives

Magnification

Immersion

NA

1.25x

Air

0.04

10x

Air

0.32

20x LWD

Air

0.4

40x LWD

Air

0.6

40x

Oil

1.3

63x

Oil

1.4

This automated inverted microscope has multiple features to enable long-term time lapse imaging experiments: a LiveCell enclosed chamber that permits temperature, CO2, and humidity control; adapters for well plates, dishes and slides; and a Ludl motorized stage and focus motor that can be used to mark multiple sites in xyz. Images are captured with a Q Imaging Retiga 2000R cooled CCD camera; 1600 X 1200 pixels, 7.4µm X 7.4µm per pixel, sensitivity is high and linear through ~550nm and then decreases linearly between 600nm to ~900nm. Exposure to light is controlled by Uniblitz shutters. The system is controlled by MetaMorph software. An on-site tissue culture incubator is available to house cells prior to imaging. Typical uses for this system include cell tracking, neurite growth, cell motility, wound healing, and Fura-2 imaging.

Under a microscope

At left, Olympus Live Cell System. At right, images of the same specimen at increasing magnification taken by Crystal Pristell

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

D350/50x

83100bs

S450/36m

FITC

S492/18x

83100bs

S530/35m

Texas Red

S572/23x

83100bs

S630/60m

CFP

S430/25x

51017bs

S470/30m

YFP

S510/20x

51017bs

S535/30m

Cy5

S655/20x

86020bs

S710/75m

FURA2*

ET340x ET380x

T400LP

ET510/80m

Objectives

Magnification

Immersion

N.A.

5X

air

0.15

10X

air

0.3

20X

air

0.45

40X

air

0.6

60X

oil

1.42

20X*

air

0.5

The Axioplan 2ie is outfitted for studies of samples under polarized light. Standard glass slides can be examined under linear and circularly-polarized light, and basic brightfield is available as well. The AxioCam MRc camera coupled with AxioVision software make this a simple-to-use polarized light imaging system.

Lamb hoof stained with picosirius red by Virginia Gillespie

Axioplan2 microscope is equipped with optics for brightfield (left), plane polarized (center) and circularly polarized (right) light microscopy. Lamb hoof stained with picosirius red by Virginia Gillespie.

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

AT350/50x

T400LP

ET460/50m

GFP

ET470/40x

T495LPXR

ET525/50m

Texas Red

ET560/40x

T585LPXR

ET630/75m

Cy5

ET620/60x

T660LPXR

ET700/75m

CFP

ET436/20x

T455LP

ET480/40m

YFP

ET500/20x

T515LP

ET535/30m

Spectrum Orange*

HQ548/20x

Q565LP

HQ580/14m

Objectives

Magnification

Immersion

NA

10x

air

0.3

20x

air

0.75

40x

air

0.75

40x

oil

1.3

63x

oil

1.4

100x

oil

1.4

*available on request

This manually-controlled Olympus stereoscope is ideal for imaging whole organs or even entire embryos stained with brightfield or fluorescent markers. The system features a dual-chip Olympus DP80 camera that allows researchers to easily switch between a color sensor for brightfield imaging and a monochrome sensor for higher-sensitivity fluorescence imaging. Larger, tiled fields can be built manually by the user. A 10x zoom factor coupled with optional 0.63x, 1.0x and 2.0x front lenses permits a very large range of magnifications for imaging. The MVX10 is controlled by Olympus' cellSens software suite.

Olympus stereo microscope

At left, Olympus Macroscope. At right, skeletal prep from an E16.5 mouse stained with Alizarin Red (bone) an Alcian Blue (cartilage) by Jianyun Yan.

Fluorescence Filters

Fluorophore

Excitation

Beam split

Emission

DAPI

AT350/50x

T400LP

ET460/50m

GFP

ET470/40x

T495LPXR

ET525/50m

DsRED

ET545/25x

T565LPXR

ET605/70m

CY5*

ET620/60x

T660LPXR

ET700/75m

CFP*

ET438/20X

T450LPXR

ET518/20m

Objectives

Magnification

Immersion

NA

0.63x*

air

0.15

1x

air

0.25

2x

air

0.50

*available on request