Instruments and Services

The Microscopy CoRE is comprised of laser scanning confocal, spinning disk confocal, multi-photon, electron, and widefield microscopes as well as image processing workstations –which are open every day, including holidays at any hour of the day. The CoRE offers full-service Transmisison-Electorn Microscopy, provides imaging support, and can guide users through image-analysis strategies. CoRE scientists can also collaborate with investigators to develop novel imaging and analysis approaches.

Instruments

 

Widefield

  • Zeiss Axio Imager.Z2 (2x) with motorized XYZ movement
  • Leica DMi8 for live cell microscopy
  • Olympus MVX10 macroscope
  • Zeiss Axioplan 2ie for polarized light microscopy
  • Olympus IX71 for simple screening

Confocal

  • Superresolution: Leica TCS SP8 with STED 3x and White Light Laser
  • Superresolution: Zeiss LSM 880 Airyscan with 34-channel spectral detector
  • Leica TCS SP8 with spectral detector
  • Zeiss LSM 780 with 34-channel spectral detector
  • Yokogawa Spinning Disk, built on Zeiss Axio Observer.Z1

Multiphoton

  • Olympus FVMPE-RS-with Dual Line Insight X3 laser
  • Olympus FV1000 with Coherent Cameleon Vision II laser (this must be added)

Lightsheet Microscope

  • LaVision Biotec UltraMicroscope II (zoom and infinity optics available)

Transmission Electron

  • Hitachi 7500
  • Hitachi 7700

Applications

  • Whole organ/organism live/fixed imaging
  • Whole organ/organism fluorescence imaging
  • Superresolution confocal imaging
  • Spectral unmixing for multiplexed samples
  • High-resolution widefield with deconvolution processing available
  • Fast, high-resolution live-cell imaging with Yokogawa Spinning Disk
  • FLIM and FRET