At the Icahn Institute and Department of Genetics and Genomic Sciences, we use next-generation sequencing platforms to create a genome-wide view of biology. Below are services currently available from our genomics facility:
|
Genetic Event/Application |
Method |
Available Technology |
|---|---|---|
|
Single molecule sequencing |
Amplification free library preparation |
PacBio RS |
|
5-Methylcytosine |
Methylation arrays Bisulfite sequencing |
Illumina BeadArray Illumina HiSeq 2500 PacBio RS |
|
Discovery of novel epigenetic modifications |
Direct observation of polymerase kinetics |
PacBio RS |
|
Whole-genome sequencing for organisms with no genome assembly (bacteria, viruses, non-model organisms) |
Amplification-free 2kb library preparation Illumina gDNA library preparation |
PacBio RS Illumina HiSeq 2500 |
|
Somatic and germ line mutations, deletions, genomic rearrangements and CNVs |
Genotyping by microarray Whole-Exome sequencing Amplicon sequencing |
Illumina BeadArray Illumina HiSeq 2500 PacBio RS |
|
Chromatin structure and in-vivo protein/DNA interactions |
Chromatin Immuno-precipitation and library preparation ("ChIP-Seq") |
Illumina HiSeq 2500 Illumina MiSeq |
|
Plasmid/Amplicon Sequencing |
Sanger dye-terminator sequencing |
AB 3730xl Capillary Sequener |
|
Gene expression |
cDNA library construction ("RNA-Seq") cRNA direct Hybridization |
Illumina HiSeq 2500 PacBio RS |
|
Alternative splicing |
cDNA library construction followed by junction analysis |
Illumina HiSeq 2500 PacBio RS |
|
Small RNA profiling and discovery |
RNA adapter ligation and PCR |
Illumina MiSeq Illumina HiSeq2500 |