The CellInsight CX7 LZR HCA platform is a laser-based, high-content imaging system that automates everything from imaging to data extraction. Equipped with seven solid-state lasers (405nm, 450nm, 488nm, 561nm, 594nm, 647nm, and 785nm) and Olympus objectives (4x/10x/20x/40x/60x; highest NA=0.90), the system is capable of fast widefield imaging, spinning disk confocal imaging for improved optical sectioning, and brightfield imaging for sparse wells. Pre-calibrated sample carrier templates and fast laser autofocus minimize setup time for the user. The instrument is used most often to image samples in glass-bottom well plates ranging in size from 6 to 1,536 wells, and it is also compatible with standard microscope slides.
The platform is integrated with ThermoFisher’s HCA Studio. This proprietary software package enables image analysis and data extraction to be carried out concurrently with imaging, even feeding analysis data back into the ongoing acquisition to improve results. Offline workstations (one in Hess and a second in Annenberg) can be used for additional data analysis. Users may also choose to export images and analyze them using any other software package they require.