LaVision Light Sheet Microscope

The state of the art UltraMicroscope II is the second generation of light sheet microscope presented by LaVision BioTec. The homogeneous single plane excitation is achieved by the bidirectional triple light sheet technology while the fluorescence signal is detected by a sCMOS camera perpendicular to the illumination plane. The selective plane illumination strategy offers excellent optical sectioning capabilities, and reduces fluorophore bleaching significantly. As a result, a 3D image stack of cleared whole mount organs can be generated in reasonable time. UltraMicroscope II imaging is compatible with both organic solvent and aqueous buffer.

Left, mouse embryo imaged by Evan Bardot; Middle, UltraMicroscope II; Right, mouse brain imaged by Korey Kam.

Technical Specifications

Laser Lines EX

Filter EM

488 nm

525/50 nm

561 nm

595/50 nm

640 nm

670/50 nm

785 nm

845/55 nm


Objectives

Magnification

Immersion

N.A.

Working dist (mm)

1.3X *

air

0.08

9

4X *

immersion

0.3

5.6

12X *

immersion

0.53

8.5-10.5


* available upon request