Lightsheet Microscopy
The state-of-the-art UltraMicroscope II is the second generation of lightsheet microscope developed by LaVision BioTec. The homogeneous single plane excitation is achieved by the bidirectional triple lightsheet technology while the fluorescence signal is detected by a sCMOS camera perpendicular to the illumination plane. The selective plane illumination strategy offers excellent optical sectioning capabilities and reduces fluorophore bleaching. As a result, a 3D image stack of cleared whole-mount organs can be generated in reasonable time. UltraMicroscope II imaging is compatible with both organic solvents and aqueous buffers.
Technical Specifications
|
Laser Lines EX |
Filter EM |
|
488 nm |
525/50 nm |
|
561 nm |
595/50 nm |
|
640 nm |
670/50 nm |
|
785 nm |
845/55 nm |
Objectives
|
Magnification |
Immersion |
N.A. |
Working dist (mm) |
|
1.3X * |
air |
0.08 |
9 |
|
4X * |
immersion |
0.3 |
5.6 |
|
12X * |
immersion |
0.53 |
8.5-10.5 |
* available upon request
Lifecanvas SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed. The axial sweeping technology allows for generating homogenous axial PSF across the entire field of view. Illumination optics scan the beam while synchronized to the camera’s rolling shutter detection. As a result, a 3D image stack of cleared whole-mount organs can be generated (whole mouse brain with 1 color channel 1.625x in <10 min, 3.6x in < 45 min). Available laser lines are 488,561, 647, and 785nm. Objective lenses with magnifications 1.65X, 3.6X, and 9X are available for imaging.
Post-processing is available: Computational streak/ stripe removal (pystripe) and Stitching (Terastitcher). Please contact the Microscopy Core Team to check the clearing method and imaging buffer compatibility.
