LaVision Light Sheet Microscope

The state-of-the-art UltraMicroscope II is the second generation of lightsheet microscope developed by LaVision BioTec. The homogeneous single plane excitation is achieved by the bidirectional triple lightsheet technology while the fluorescence signal is detected by a sCMOS camera perpendicular to the illumination plane. The selective plane illumination strategy offers excellent optical sectioning capabilities and reduces fluorophore bleaching. As a result, a 3D image stack of cleared whole-mount organs can be generated in reasonable time. UltraMicroscope II imaging is compatible with both organic solvents and aqueous buffers.

LightSheet Ultramicroscope II

 

Technical Specifications

Laser Lines EX

Filter EM

488 nm

525/50 nm

561 nm

595/50 nm

640 nm

670/50 nm

785 nm

845/55 nm


Objectives

Magnification

Immersion

N.A.

Working dist (mm)

1.3X *

air

0.08

9

4X *

immersion

0.3

5.6

12X *

immersion

0.53

8.5-10.5


* available upon request

User Protocol