The state-of-the-art UltraMicroscope II is the second generation of lightsheet microscope developed by LaVision BioTec. The homogeneous single plane excitation is achieved by the bidirectional triple lightsheet technology while the fluorescence signal is detected by a sCMOS camera perpendicular to the illumination plane. The selective plane illumination strategy offers excellent optical sectioning capabilities and reduces fluorophore bleaching. As a result, a 3D image stack of cleared whole-mount organs can be generated in reasonable time. UltraMicroscope II imaging is compatible with both organic solvents and aqueous buffers.
Technical Specifications
|
Laser Lines EX |
Filter EM |
|
488 nm |
525/50 nm |
|
561 nm |
595/50 nm |
|
640 nm |
670/50 nm |
|
785 nm |
845/55 nm |
Objectives
|
Magnification |
Immersion |
N.A. |
Working dist (mm) |
|
1.3X * |
air |
0.08 |
9 |
|
4X * |
immersion |
0.3 |
5.6 |
|
12X * |
immersion |
0.53 |
8.5-10.5 |
* available upon request