The Department of Stem Cell Biology and Regenerative Medicine collaborates closely with core facilities maintained by the Dean’s Centers of Research Excellence (CoREs):
Stem Cell Engineering CoRE
The mission of the Stem Cell Engineering CoRE (or SCEC) is to support researchers across different departments to implement human models into their research programs.
The SCEC is centrally located in Icahn Building 13th floor; it also has a laboratory for cell production and gene editing in the Discovery and Innovation Center in Midtown West. The SCEC provides human pluripotent stem cell (PSC) lines, as well as laboratory space, validated reagents, and hands-on training for scientists.
Services include iPSC generation from somatic cells such as blood and fibroblasts, extensive characterization, and long-term banking. We also provide access to well-characterized iPSC lines derived from both healthy individuals and patients with diverse diseases, spanning different ethnic backgrounds and sexes. A major focus of the SCEC is gene editing in PSCs, offering a wide range of CRISPR-based services—from point mutation knock-in (correction or introduction) to the generation of reporter PSC lines (e.g., GFP or other reporters), as well as inducible systems such as Cre-lox, dTAG, and more. In addition, the Core provides assistance with differentiation protocols of PSCs into various cell types, both in monolayer cultures and in 3D organoid systems.
Transgenic and Genome Editing CoRE
The Transgenic and Genome Editing CoRE provides mouse model creation and related services for Mount Sinai investigators and external customers.
The facility offers full model creation services, from consultation until delivery of germline-confirmed genetically engineered mouse models (GEMMs). We employ the latest genome editing tools such as CRISPR/Cas9 and the assisted reproductive technology to rapidly generate GEMMs with diverse genome modifications. In addition, we continue to support model creation via the traditional transgenesis and the gene targeting in the embryonic stem cells
Flow Cytometry CoRE
The Flow Cytometry CoRE facility provides the Mount Sinai research community with access to state-of-the-art flow cytometry and cell sorting instrumentation. The core supports experimental design, data acquisition, analysis, and training for a wide range of cytometric applications.
Microscopy CoRE
The Microscopy CoRE facility provides the Mount Sinai research community with access to state-of-the-art optical and electron microscopy systems. The core supports high-resolution imaging, sample preparation, image analysis, and expert consultation to enable cellular, subcellular, and structural studies across basic and translational research.