The Icahn Institute for Genomics and Multiscale Biology has made a substantial investment in high-throughput processing equipment for large BeadArray projects. We operate two TECAN Evo liquid handling robots, capable of processing 24 chips per run. Our Illumina HiScan microarray scanner is a top-of-the-line system with a robotic arm that allows for 24-hour microarray data acquisition. With the existing equipment and facilities, the genomics core can process up to 600 samples per week.
If you would like to submit samples for BeadArray analysis, please see sample submission page for location and pricing. Guidance for experimental design is also available.
DNA microarrays are a well-established technology for genome-wide characterization of gene transcription, single nucleotide variation, copy number variation, and epigenetic cytosine methylation. Our genomics core employs the most robust and accurate method on the market––the Illumina BeadArray platform.
Traditionally, microarrays were glass slides printed with short DNA strands. This method has serious drawbacks such as uneven spot morphology, signal bias due to spot position, and low design flexibility. BeadArrays are a unique approach to microarray technology, using glass slides with micrometer-sized holes to house oligonucleotide-coated beads. The BeadArrays are used as a standard array would be, with the exception of a molecular decoding step that is performed by the scanner.
The BeadArray platform can be used for genotyping, gene transcription quantification, and cytosine methylation.
Human genotyping studies are most often performed using the Infinium HD using OmniExpress (750k SNPs) or Omni2.5-8 arrays (2.4M SNPs). These chips are also capable of copy number variant estimation (CNV). Custom content chips for Genome Wide Association Studies (GWAS) are also available. For more information, please contact us with details of your project.
Gene expression analysis chips are available for humans, mice, and rats. All chips contain all known genes and many regulatory RNAs. As of March 2012, microRNA chips and custom gene expression services have been discontinued by Illumina.
Genome-wide methylation scanning in human samples (including stem cells and tumor cells) can be accomplished with the human methylation 450K array. This array covers CpG islands, sites known to be methylated in promoters, DNase hypersensitive sites and miRNA promoter regions.
Learn more about formalin fixed, paraffin embedded, samples that can be used for genotyping studies