The Biorepository and Pathology CoRE is a multi-dimensional service group providing consultation and technical support for the translational and basic science groups across the Mount Sinai Health System.
If you have any questions regarding these services, please contact Rachel Brody, MD, PhD at rachel.brody@mountsinai.org .
Please note: it is important that you acknowledge the Biorepository and Pathology CoRE in all publications and grants that include data derived from our facility.
The Core provides facilities for the processing and banking of various hematologic tissues. Blood samples may be stored as whole blood or processed to yield specific blood fractions. Downstream applications include cytokine/chemokine studies, mass spectrometry, various nucleic acid studies (sequencing, microassays, etc.), immunologic studies, stimulation assays and expression studies. Samples are processed in BSL II facilities utilizing sterile technique and may be coded and de-identified as per individual studies. Tissues are stored at -80C freezers or vapor phase liquid N2. All audit trails may be traced with our secure databases. Tissues/cells that may be collected include:
For more information, please email Maryann Huie, PhD (maryann.huie@mssm.edu) or call at 212 241-2248
We offer basic histology services, including processing, embedding and sectioning of frozen and fixed (human and animal) tissue samples. We provide hematoxylin and eosin stained slides, as well as multiple special stains (e.g. Acid Fast Bacilli (AFB), Periodic Acid Shift (PAS), Giemsa, Gram, Masson’s trichrome etc...). We can generate unstained sections for a variety of applications, such as immunohistochemistry, in situ hybridization, laser capture microdissection, etc.). We also offer the generation of tissue microarrays (TMA) from different tissue blocks.
Equipment
If you have any questions regarding these services, please email Fran Avila or Alan Soto, or call them at 212-241-3539.
The Pathology CoRE provides a variety of immunostaining services, including immunohistochemistry and immunofluorescence using commercial antibodies. We perform both semi-automated and full-automated (Discovery Ultra staining platform, Roche). We also provide antibody characterization/titration/interpretation and imaging services, as well as rapid immunohistochemistry staining for laser capture microdissection.
Equipment
Discovery Ultra (Roche): Automated Immunohistochemistry (IHC)/In situ hybridization (ISH) research slide staining system. For research application including IHC, multiplex Immunofluorescence, DNA / RNA ISH
If you have any questions regarding these services, please email Tin Htwe Thin/ Monica Garcia-Barros or call 212-241-6050 or at 212-241-2148.
The Pathology CoRE provides laser capture microdissection service from frozen and/or paraffin-embedded tissue samples as well as cells. We also offer training to all investigators interested in using the system. We can provide sectioning of samples onto special membrane-coated slides and rapid immunostaining as required.
Equipment
Leica Microsystems LMD6500 Laser Microdissection: For specimen collection by gravity contact, contamination-free to obtain ultra-pure sample of single cells, both from tissue samples and cells.
If you have any questions regarding these services, please email Tin Htwe Thin or call 212-241-6050.
Whole Slide Scanning (Bright-Field)
The core uses a Hamamatsu NanoZoomer S210 Digital Slide Scanner to perform whole slide scanning services from whole tissue sections, tissue microarrays, cytology specimens, etc.
The scanner provides bright-field image capture at 20X and/or 40X magnification. The system includes NDP.view2 Viewing software.
If you have any questions regarding scanning service, please email or call Alan Soto at 332-215-2965 or Monica Garcia-Barros at 212-241-2148.
IHC Imaging
The Biorepository and Pathology CoRE offers quick imaging of IHC stained slides using Nikon EclipseCi-L upright clinical microscope.
Nikon Eclipse Ci-L offers bright-field image capture at 4X, 10X, 20X, 40X and/or 60X magnification. If you have any questions regarding imaging service, please email Tin Htwe Thin/ Monica Garcia-Barros or call 212-241-6050 or at 212-241-2148.
HALO from Indica Labs - Image Analysis
The Pathology Core offers quantitative tissue image analysis using HALO® Image Analysis Platform (Indica Labs). The platform offers 5 different modules:
If you have any questions regarding image analysis service, please contact Tin Htwe Thin (tinhtwe.thin@mssm.edu ; 212-241-6050) and Monica Garcia-Barros (monica.Garcia-barros@mssm.edu ; 212-241-2148)
Molecular DNA/RNA Extraction
The Pathology CoRE provides DNA and RNA (total/miRNA) extraction including quantitation/ quality services from a variety of specimen sources including blood, buffy coat, platelets, plasma exosomes, PAXgene, frozen/fixed (FFPE) tissues prepared by micro-dissection (LMD) or macro-dissection (scroll/punch/whole or partial scrape off), swabs, spit cups, brushes, cytofluids, and more. Free consultation is available.
Equipment
For more information, please email Yayoi Kinoshita, DMD or call her at 212-241-4414.
The Biorepository is responsible for collecting, storing, processing, and distribution of human tissue (fresh, frozen, FFPE) and body fluids (blood, urine, and cerebrospinal fluid)
Our mission is to provide high-quality annotated specimens with the potential to be linked (with appropriate IRB approval and patient consent) to clinical and pathological data.
Specimens are readily available for research and open to all investigators within the Mount Sinai Health System, as well as to outside investigators.
Samples can be collected from Mount Sinai Hospital and affiliates.
To request specimens from the Biorepository please contact Rachel Brody, MD PhD (Rachel.Brody@mountsinai.org ; 212-241-0678; ext. 40678)
A project BRC# will be assigned provided the following criteria are elucidated and placed on file:
1.An abstract or specific aims that clearly describe the project for which you will use human tissues or fluid, including a detailed description of collection requirements; grant support information for your research program; assurance of institutional IRB approval, GCO fund number.
2.Completed forms acknowledging responsibility in understanding of and adherence to appropriate safety standards for the protection of yourself and others while handling human tissues; acknowledging that you will not further distribute the materials disbursed to you by the Biorepository
3.Agreement to provide specific acknowledgement to the Mount Sinai Biorepository in any publications or grants related to the use of the tissues you receive.