The Metabolomics Core at the Icahn School of Medicine at Mount Sinai is a newly established facility located at the Mount Sinai West campus. Our mission is to support our investigators in their research in the study of metabolism by providing state-of-the-art mass-spectrometry-based metabolomics assays. Our services are also available to investigators from other academic institutions and commercial enterprises.

Our facility offers several targeted and untargeted GC/MS- and LC-MS-based analysis that are validated against a wide range of sample types, including body fluids (plasma, serum, saliva, urine), cells, and various tissue types from human subjects and research animals. We are also experienced in performing stable isotope tracing studies using 13C, 15N, and 2H-labeled substrates. From the experimental design stage through data acquisition, data analysis, and interpretation, we support hypothesis-generating untargeted and hypothesis-driven targeted metabolomics studies.

Our equipment and service fees are as follows:

• Agilent 8890/5977C GC-MS
  • Relative quantification of amino acid and TCA) cycle: $50 per sample
  • Stable isotope tracing of 13C to TCA cycle: $65 per sample
• Agilent 6495D QqQ LC-MS/MS
  • Polar metabolomics profiling (250 compounds panel): $75 per sample
• Thermo Scientific Orbitrap ID-X
  • Untargeted metabolomics profiling: $105 per sample
  • Stable isotope tracing: $120 per sample

Please note that a new protocol submission request must be initiated in iLab for every new project. Samples will not be accepted without an active service request. Users are encouraged to contact us for instructions on how to register and submit a service request through iLab. Likewise, the raw data for each project belongs to the researcher. Researchers are responsible for obtaining and storing their data files.

Acknowledgement of CoRE in Publications, Grants, and Awards

Acknowledgement of the Metabolomics CoRE is crucial to supporting our ongoing operations and development, as well as our acquisition of future instrumentation and capabilities. Through publication lists, we demonstrate impact, support grant proposals, and comply with reporting requirements.

We ask that any work done with Metabolomics CoRE that produces data in a publication be acknowledged as: "Metabolomics Core, Shared Research Resources, Icahn School of Medicine at Mount Sinai" in the publication. Furthermore, if CoRE staff provide significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the principal investigator), it is expected that these individuals will be co-authors on the publication.

We recommend users follow the Association of Biomolecular Resource Facilities Recommended Guidelines for Authorship on Manuscripts when and where acknowledgement is appropriate.