Flow Cytometry Dean's CoRE | Icahn School of Medicine

DEAN’S CORES

Flow cytometry measures high-order multidimensional data with single cell granularity. It acquires these measurements at rates of thousands per second and works with virtually unlimited population sizes, offering outstanding statistical applications. To leverage this data and return a highly purified subpopulation of live functional cells is the power of cell sorting.

Situated within the Icahn School of Medicine at Mount Sinai, our CoRE provides all members of the Mount Sinai Health System community with the instrumentation, expertise, and education required to incorporate flow cytometry and cell sorting into any research project. To further fuel scientific research, we monitor and introduce new flow cytometry equipment, methods, and data analyses approaches on a regular basis.

Explore the Flow Cytometry CoRE

Step 1: Generate an iLab Account

Visit the Mount Sinai iLab sign-in page.
Note: You must have a linked fund to proceed with reservations.

Step 2: Email the Core Contact us at FlowCytometryCoRE@mssm.edu and provide the following details:

Your lab location (e.g., Icahn, Hess, etc.).
Your availability over the next few business days.

Step 3: Consultation We will schedule a 30-minute session to cover:

Protocol discussion and experimental design.
Instrument selection (e.g., conventional vs. spectral).
An introduction to the Flow CoRE iLab management system.

Step 4: Training Training requirements are based on your experience level:

Experienced Users: Maintenance and instrument-specific refresh training (includes startup, shutdown, cleaning and data backup).
New Users: Complete comprehensive training on the selected instrument.

The Flow Cytometry CoRE is equipped with eight flow cytometers and nine fluorescence activated cell sorting machines in several locations across the Icahn School campus. All equipment is maintained daily by CoRE staff.

Analyzers

Sorters

Icahn Medical Institute, 13th Floor

· Thermo Attune (2018, four lasers, 14 detectors)

· Cytek Aurora (2019, five lasers, 64 detectors)

· Amnis ImageStream (2010, six lasers, 12 detectors)

· BD FACSymphony A5 (2023, five lasers, 50 detectors)

Icahn Medical Institute, 13th Floor

· BD FACSymphony S6 (five lasers, 23 detectors)

· Cytek AuroraCS (five lasers, 60 detectors)

· Miltenyi Tyto (three lasers, eight detectors)

· BD FACSDiscover S8 (five lasers, 78 detectors)

Mount Sinai Discovery and Innovation Center, Sixth Floor

· Cytek Aurora (2023, five lasers, 64 detectors)

Hess Center for Science and Medicine, Fifth Floor

· BD FACSymphony S6 "I" (five lasers, 23 detectors)

· BD FACSymphony S6 "II" (five lasers, 23 detectors)

Annenberg Building, 14th Floor

· Cytek Aurora (2023, five lasers, 64 detectors)

Hess Center for Science and Medicine, Sixth Floor

· BD LSRFortessa "A" (five lasers, 18 detectors)

· BD LSRFortessa "B" (five lasers, 18 detectors)

· Thermo Attune (four lasers, 14 detectors)

Analyzers (Aurora, Fortessa, CantoII, Attune, A5): $63/hr (Self-Op) | $139/hr (Assisted)
ImageStream: $100/hr (Self-Op) | $150/hr (Assisted)
Sorters (S6, CS, S8): $122/hr (Self-Op) | $168/hr (Assisted)
Tyto: $126/hr (Assisted) + $140/cartridge
Data Analysis: $158/hr

Booking & Cancellation Policy

Minimum Booking: 1 hour for Sorters; 30 minutes for Analyzers and Tyto.
Advance Notice: Sorters and Tyto must be booked at least 5 hours prior.
Late Cancellations: Cancellations made less than 24 hours before a session incur a 70% charge of the booked time.
Rebooking Credit: If your canceled slot is rebooked by another user, the original slot will not be charged.

Users are responsible for the immediate collection and backup of their data following acquisition.

The Flow Cytometry CoRE does not provide long-term data storage or archival services. Data on acquisition computers will be deleted on a weekly basis to ensure smooth instrument operation.

Mount Sinai IT provides multiple secure data storage options (e.g., network drives, OneDrive, Teams, Google Drive):

For guidance on selecting the appropriate solution, please contact Sinai IT.

We are required to acknowledge NIH funding sources in all publications (manuscripts, abstracts, presentations, etc.) that utilize our resources. Please use the following wording in every publication resulting from the use of the Flow Cytometry CoRE instruments:

Acknowledgment Text: "Research reported in this publication was supported by the Flow Cytometry CoRE (RRID:SCR_027701) at the Icahn School of Medicine at Mount Sinai and by the National Cancer Institute of the National Institutes of Health under award number P30 CA196521. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."

Please notify the CoRE administration once your manuscript has been accepted for publication by contacting Siu-hong Ho and Shekhar Patil.