The Grant Application Resource Center (GARC) provides standardized language and links to information to support the development of grant applications. Use this section to obtain standardized language describing research related institutional resources, plus other guidance and recommended language for use in specific types of grant submissions.
Grant Application Research
The Center for Comparative Medicine and Surgery (CCMS) is a centralized, shared resource supporting animal research via veterinary, husbandry care, and research collaboration with the faculty at Icahn School of Medicine at Mount Sinai. The program has been fully accredited by the Association for Assessment & Accreditation of Laboratory Animal Care, International (AAALAC - #00002), since 1967. The center also provides assistance with research protocol design, clinical and anatomic pathology, and training of research personnel in the care and use of laboratory animals. There are four primary sites of operation, with the central administration located in the Annenberg Research Building, 26th Floor. The other major housing areas are the Icahn Medical Institute (IMI); the Hess Center for Science and Medicine (HCSM) and the Atran Research building.
Annenberg Research Building
The largest variety of species is housed in this building. Administrative offices, large animal surgical support (one operating room and two catheterization labs), and a veterinary diagnostic laboratory are located here.
Icahn Medical Institute
This state-of-the-art rodent barrier facility offers fully automated rodent housing (ventilated caging and automatic watering), necropsy suite, and Animal Biosafety Laboratory (ABSL2/3) capability. Also located within this vivarium are the Mouse Genetics Shared Research Facility, Metabolic monitoring facility, and Gnotobiotic rodent facility.
Atran Research Building
This small facility provides overflow support for the Annenberg and IMI, respectively. Animals housed in this area reflect the general health profiles of the major support areas.
Hess Center for Science and Medicine
The HCMS vivarium is the newest and largest vivarium on campus. Designed to specifically support Imaging, Neuroscience behavioral studies, and Cancer research, the vivarium is adjacent to the Translational and Molecular Imaging Institute’s small animal imaging suites and large animal imaging capability. Fully automated, robotics, cage wash support and state-of-the-art environmental monitoring systems are additional key features of this facility.
The faculty of the CCMS consists of four full time veterinarians: (Director, DVM/DACLAM; Associate Director and Head, Veterinary Translational Medicine and Director of Behavioral Management Program, DVM/DACLAM; Head, Comparative Surgery Facility, DVM and Veterinary Pathologists, DVM). The Husbandry operation is managed by an Operations Manager and four full time Supervisors. All are certified at the Laboratory Animal Technologist level LATg) by the American Association of Laboratory Animal Science (AALAS). An ongoing in-house training and post-approval compliance program is in place and conducted by a full time Training/Compliance Coordinator (MS/LATg). The institution also supports a weekly AALAS certification training program for all husbandry and research personnel interested in certification.
Last Update: March 2019
The Clinical Research Unit (CRU) is the hub for conducting clinical and translational research at the Icahn School of Medicine at Mount Sinai. There are more than 100 protocols being performed from a wide variety of specialties and subspecialties involving research participants of all ages. The staff includes nurse practitioners who can perform physical examinations, monitor participants, and perform infusions, and medical assistants who perform phlebotomy, electrocardiograms and process samples. The reception area, located on the ground floor of the Annenberg Building (adjacent to the elevator bank), includes exam rooms for brief visits and minor procedures. The Ross Park CRU Annex is equipped with rooms for day-long procedures and interventions such as infusions and PK sampling. Resources are available for specimen processing as well. The CRU is open Monday - Friday from 8:00 AM - 5:30 PM. Unscheduled, walk-in phlebotomy services are available weekdays 9:00 AM to 4:30 PM.
Last Updated: March 2019
The Department of Pharmacy maintains an Investigational Drug Service (IDS) to support research activities at Icahn School of Medicine. The IDS is a research pharmacy charged with maintaining the control and accountability of investigational agents (FDA approved and non-approved) in compliance with Good Clinical Practices (GCPs), Good Manufacturing Practices (GMPs) and other regulations and laws as appropriate. The IDS provides dedicated research support including protocol review, storage, preparation, dispensing and education to groups and investigators engaged in research.
The IDS sets up specific procedures and systems for each clinical trial to assure maximum benefit and safety for patients enrolled in studies. The clinical coordinators are available to consult with any investigator or sponsor to discuss the logistics of conducting their trial at Mount Sinai. All human subject trials requiring the use of medications must get authorization from the IDS prior to Program for the Protection of Human Subjects (PPHS) final approval (IDS and PPHS reviews occur concurrently).
Last Updated: March 2019
Mount Sinai Data Warehouse (MSDW) provides data access for planning and executing clinical and translational research as well as for quality of care and process improvement initiatives.
MSDW collects and organizes clinical, financial and operational data derived from patient care processes of The Mount Sinai Hospital and Mount Sinai Doctors Faculty Practice. Data Warehouse contains detailed inpatient and outpatient data since 2003 from over 20 different Mount Sinai systems/applications for more than eight million patients and over two billion facts.
MSDW is compliant with New York State and HIPAA regulations and with Mount Sinai PPHS and IRB policies regarding protection of human subjects and participation in research.
Researchers planning new studies or grant applications can utilize our online Cohort Query Tool and quickly search the Data Warehouse to quantify eligible cohorts.
MSDW can help research studies by identifying the study population and by providing daily/weekly/monthly reports. In addition, MSDW can provide datamarts or smaller subsets of data to study cohorts.
The cost of data delivery for research studies is based on the effort required by the data warehouse team to analyze and generate the desired output. Assistance with estimating Data Warehouse costs for grant application purposes is available upon request.
Visit the Mount Sinai Data Warehouse for more information.
Last Update: March 2019
Developed in response to the needs of the Mount Sinai Health System’s (MSHS) expansion and the parallel evolving complexity of research regulations, policies and processes, the Office of Research Services (ORS) was established to facilitate the conduct of translational and clinical research through a centralized infrastructure supported by ConduITS’ Administrative Core and the Office of the Dean of Icahn School of Medicine at Mount Sinai. The ORS provides oversight of program organization, evaluation, continuous improvement, quality, efficiency, safety and communications allowing ConduITS to continually identify barriers, gaps and unmet needs of the MSHS research community and to implement integrated solutions that impact the scope of Mount Sinai’s learning healthcare system.
The ORS is staffed with 7 FTEs in offices located near the medical schools' main campus. The staff provides researchers throughout the MSHS with:
- Navigation of
- Required training for the conduct of research
- Lifecycle of research management
- Clinical Trials Management System (CTMS)
- The ISMMS Offices of Research Administration
- Human Subjects Protections Program
- Grants and Contracts Office
- Investigational Drug Services
- Research Compliance
- Research Financial Conflicts of Interest
- Financial Administration of Clinical Trials
- Regulatory Coordination:
- Preparation of internal and external regulatory documents
- Investigational New Drug Application and Investigational Device Exemption (IND/IDE) submissions & progress reports to the FDA
- Centralized ClinicalTrials.gov registration, updates and reporting
- Grants Application Resource Center
- Research Orientation
- Clinical Research Coordinators Bootcamp
- Individual meetings with new faculty
- On-demand requests for research team orientations
- Consulting Services
- ClinicalTrials.gov registration, maintenance & reporting
- Pre-protocol development
- Multi-Site Study support
- Research recruitment strategies
- ResarchMatch.org support
- Training, Education & Communications
- Customized education & training to fill knowledge gaps
- Research Listserv
- Research Digest – quarterly publications
Given the increasing need for innovative informatics solutions to managing the workflow for translational sciences across the MSHS, the ORS has developed e-tools to assist researchers find answer to questions and provide navigation of the MSHS research infrastructure. These platforms also collect metrics about utilization, issues and customer satisfaction to assist in the continuous improvement of the services provided by the ORS. In 2018, the ORS launched the health system’s global research help desk, Research 411, which served approximately 1,000 individuals. The ConduITS Research Roadmap (RRM) will be implemented in Q2 2019. The RRM is a web-based, interactive, real-time tool that provides MSHS researchers with navigation of the health system’s research operations, workflows, resources and imbeds real-time and evolving regulatory guidance and best practices to provide the highest quality conduct of research at the MSHS. The RRM also provides a centralized, dedicated environment for ConduITS to communicate advances to the network of CTSA hubs across the nation. The ORS will also lead the implementation of the institution’s system-wide clinical trials management system which will further streamline research study conduct, oversight and efficiency locally and remotely across an ever-expanding health system and collaborating institutions.
Research 411 e-Portal – Research 411 is a centralized, electronic portal for general research help desk questions, information, request for services and consultations and the collection of metrics for program evaluation of quality and efficiency system-wide. It is built with JIRA software which allows the ORS team to track communications in centrally accessible message threads for continual cross-coverage of issues, increasing productivity and eliminating down-time. The program is customizable to allow for the creation of on-demand tickets for emerging topics. Additionally, the program provides information about the volume of incoming tickets in a specific area or topic. This information is then used by the ORS team to identify where there is a gap, bottleneck and/or need for education and training and provides the ability to spotlight issues and locate solutions (see Research Roadmap below). In its inaugural year Research 411 processed over 750 inquiries, in the areas of IRB, GCO, IND/IDE support, Clinicaltrials.gov, and for case-by-case queries requiring tailored support and guidance.
Regulatory Support Knowledge Base (RSKB) – (self-serve support function) a feature of Research 411 is the ability to develop an organized repository of the solutions developed through the Research 411 portal. In essence, this becomes a Frequently Asked Questions (FAQ) self-help function of the ORS where research teams can access “articles” based on targeted solutions to issues local to MSHS, general best practices and knowledge gained from the questions and solutions provided through Research 411. Articles will be organized under categories and subcategories ensuring ease of use and search capability. The RSKB will improve efficiency and investigator productivity by eliminating the need to respond to redundant issues and providing instant access to solutions. The number of ORS developed RSKB articles, the volume of users accessing them and automated customer satisfaction surveys will be used as key performance measure when evaluating RSKB.
Research Project Navigators (RPNs) – (in-person support function) to optimize rapid access to resources that maximize quality and efficiency, the ORS program will further develop dedicated RPNs with expertise in the areas of; 1) Regulatory Knowledge, 2) Quality and Efficiency, 3) Grants and Contracts, 4) Workforce Development 5) Community and Collaboration and 6) General Research Infrastructure navigation to provide targeted assistance in the areas identified as having bottlenecks and that impinge the efficiency and quality of research. The research community will request personalized consultations with RPNs through the Research 411 Portal. Working in concert with shared data through the Research 411 portal, the RPNs communicate with each other to identify and facilitate early guidance and connections for junior researchers to access the network of CTSA services to ensure safe and ethical human subject research, including the appropriateness of study design, feasibility, recruitment, timely closure of futile studies, research study workflow, analysis, and dissemination of results quality research design and good clinical practice. From this central vantage point, RPNs will also be able to assist investigators with collaborations and access to team science. The RPNs will collect, evaluate and continually improve their methods based on the data collected through Research 411, user feedback and by continully monitoring the evolving research landscape to incorporate the most current practices and regulations.
Research Roadmap (RRM) - The Research Roadmap is a robust and comprehensive web-based, centralized tool being developed by the ORS. The RRM, visually designed to function like an interactive subway map, is being developed to provide system-wide navigation of the many “pathways” of the MSHS research infrastructure and provides a “line” to the CTSA national network of resources. It is an interactive, dynamic easy to use tool that provides the most current, streamlined and efficient access to administrative processes, external regulatory requirements, internal policies, most current news, education and training opportunities toward the conduct of the highest quality research. The RRM will increase efficiency by providing information for parallel processing, monitoring of timelines and review processes. Locally designed and built, the RRM is complementary to the Research411 portal as a tangible, immediately accessible and continuously available web-based tool and platform for the dissemination of the “tips” and “best practices” learned through the Research 411 portal. The RRM increases productivity time by reducing the discovery time for research teams to understand research methods and processes. Controlled by the ORS, the team can real-time post the newest modifications and news for the system. Dissemination of RRM improvements is provided by the ORS managed research list-service and availability of local hub innovations and tools is provided through a dedicated “line” for the CTSA national network of hubs and the CTSA program. The location of solutions that are developed for gaps and bottlenecks in research systems will be more efficiently pinpointed and communicated on the RRM.
Clinical Trials Management System - The ISMMS CTSA was awarded an Administrative Supplement to accelerate the implementation of a system-wide Clinical Trials Management System (CTMS). The CTMS will provide the ability for enhance post- monitoring of ongoing clinical trials. The ORS, along with ISMMS Research IT have begun implementation with a phased roll-out starting with the Department of Medicine. The CTMS will impact rapid activation of clinical trials by enhanced protocol and informed consent life cycle management improving IRB approval duration and continuing renewal. Additionally, the CTMS’ reporting capabilities will allow for the analysis of slow or non-recruiting studies for evaluation and improvement of initial recruitment plans or study closure. The CTMS will also increase regulatory compliance with clinical research billing and Clinicaltrials.gov registration and reporting. (See Research Methods, Regulatory Support and Knowledge, Core E and Network Capacity, Core G)
Grants and Contracts Office (GCO) - The Grants and Contracts Office (GCO) is the centralized administrative office that oversees the research and sponsored project application and award process. The GCO documents policy, provides on-going information to faculty regarding sponsorship, policy changes, and funding opportunities, and generally assists faculty in all aspects of the pre-award process, including assisting with budget preparation. The GCO reviews each project for administrative and budgetary accuracy as well as for compliance with federal, state, city, and internal regulations, such as those pertaining to biosafety, human subjects, vertebrate animals, and recombinant DNA. The GCO interfaces with extramural funding agencies and will intervene on behalf of an investigator regarding funding conflicts, compliance, and subaward issues.
The GCO collects data regarding faculty research performance and reports to senior management regarding institutional performance. Information for each proposal is maintained in its database tracking system, which is the principal source of information regarding success rate and other award metrics. The GCO staff of 26 employees consists of 4 teams. Each team is led by an Authorized Organizational Representative (AORs) and includes three Grants Specialists, a Contracts Specialist, and a Grants Coordinator. Other staff members include an Education Director and a Contracts Coordinator. The AORs have the authority to endorse sponsored project applications and agreements. The GCO reviews approximately six thousand five hundred submissions per year.
Last Update: March 2019
With full accreditation from the Association for the Accreditation of Human Research Protection Programs the Mount Sinai Health System's Human Research Protection Program is a comprehensive program to ensure the protection of the rights and welfare of subjects in Human Research. The Program for the Protection of Human Subjects (PPHS) is a key component of the overall HRPP.
The PPHS supports researchers throughout the Mount Sinai Health System in assuring the ethical conduct of research and compliance with federal, state and institutional regulations and provides a professional office staff to assist investigators, participants, and Institutional Review Boards (IRBs). In addition, the overall HRPP includes organizational, administrative, and investigator components, and is based on all individuals fulfilling their roles and responsibilities. Essential aspects of the overall mission of human subjects protection are provided by the organized interaction of the PPHS with the Financial Conflict of Interest Committee, the Financial Administration of Clinical Trials Office, Investigational Drug Services, The Office of Research Compliance, the Biosafety Program and the Radiation Safety Committee.
The PPHS is a signatory to the CTSA sponsored SMART IRB platform and uses it extensively when providing single IRB functions to investigators. The PPHS is posed to provide support whether we are the IRB of record or are a relying institution.
The PPHS office offers support to investigators and their research teams through one-on-one scheduled meetings with IRB analysts to review individual applications and answer questions. The office also offers short presentations to groups, departments, or divisions on the human subjects research submission process by experienced IRB analysts. The PPHS office also offers assistance to researchers during business hours by phone, or through the general email address which is answered daily.
Last Update: March 2019
Research Data Services
Research Data Services (RDS) provides electronic data capture (EDC) tools, calendaring for specialized instrumentation. We offer two flavors of EDC tools: custom database using Sinai’s Electronic Research Application Portal (eRAP) is available with a fee covering the service; Vanderbilt’s REDCap is a free self-serving database.
RDS can rapidly develop custom databases for longitudinal (single site and multi-sites) clinical studies. Service includes reporting tools, randomization engine, data upload, and file upload. The final product that RDS delivers to you, eRAP Rapid Database Generator (eRAP RDG), is a 21 CFR Part 11 compliant and web-based interactive tool for data entry and reporting. In addition, eRAP is integrated with other Mount Sinai Health System clinical systems, such as EPIC, Mount Sinai Data Warehouse, Specimen Management Systems (Freezerworks and IPM LIMS), and SCC Labs, making sharing data between systems easy.
RDS currently serves over 1,600 users at 150 institutions in 21 countries. It hosts over 200 projects, collecting research data from over 100,000 subjects throughout the world.
As part of Scientific Computing services, we assist investigators with database design and management. Our application development consulting service will help investigators with project planning and the systems development life cycle, including requirements gathering, business process mapping, and basic system design. We also provide programming support for application development for specific database functionality based on research need.
Request database support through the Conduits Request a Service page.
Research Data Services provides support for bio-specimen collection protocols integrated with longitudinal clinical data capture. Using both in-house and third-party tools, Research Data Services can support a wide array of specimen collection and clinical annotation needs.
We support calendaring services for Mount Sinai’s Shared Research Facilities and other labs throughout Sinai. We can design a custom calendaring system that includes instrumentation-specific data for efficient scheduling of your instrument. The calendaring system can charge grants directly through Sinai Central and reconcile usage data for accurate billing. The calendar is securely accessible via eRAP.
Last Update: March 2019
All pre- and postdoctoral trainees of the Graduate School of Biomedical Sciences are required to complete training in Responsible Conduct of Research (Course Code: BSR1021).
Format: Each BSR1021 class consists of highly interactive lectures that present the subject material, augmented with case studies and videos provided by the NIH. In addition, one class on responsible publication, authorship and peer review is taught in the format of a panel discussion with Journal Editors and Study Section chairs.
Subject Matter: The subject matter is divided into 8 classes with the following topics: 1) Introduction to RCR; research misconduct and policies for handling misconduct, and safe laboratory practices; 2) mentor/mentee responsibilities and relationships; 3) Laboratory Notebooks and Data Management; 4) responsible authorship, publication and peer review; 5) policies regarding human subjects, and live vertebrate animal subjects in research; 6) Contemporary Issues in Research Ethics; 7) collaborative research including collaborations with industry; conflict of interest – personal, professional, and financial; and 8) The research misconduct review process.
Faculty Participation: Each class is team taught by faculty with expertise in the relevant topics. These include Drs: Nada Gligorov (course director and Bioethicist); Benhur Lee (Safety expert); Matthew O’Connell (Senior Assoc Dean for Curriculum, Recruitment and Admissions, mentoring, plagiarism); Eric Sobie (Senior Assoc Dean for Programmatic Development, Computation and Data Expert); Martin Walsh, Ross Cagan, Phillppe Soriano, Patrick Hof and Stephane Larochelle (Nature Publishing) - (publication/authorship/peer review panel); Paul Kenny (collaborations and conflicts); Jonathan Cohen (DVM) (Vertebrate Animal Studies); and Henry Sacks (IRB and Human research).
Duration: The course runs for a total of 8 hours over 4 weeks in the Fall semester. Attendance is mandatory. Refresher after 4 years.
Frequency: Twice per week (Monday and Wednesday)
In addition, these trainees are required to take a sister course, Rigor and Reproducibility (course code BSR 1022). This course has the same format as BSR1021, but runs in the Spring Semester.
Format: Same as BSR1021
Subject Matter: The course is split into three areas: 1) R&R at the bench (Experimental Design; The scientific premise; use of controls; hypothesis testing; biological vs technical replicates; parallel and complementary approaches); 2) Human and animal experimental design and statistical analysis (IACUC and IRB requirements; power calculations; sex as a variable; other variables including genetics; design of clinical trials; statistical testing of hypotheses and reporting of methods); 3) Digital and Quantitative Data (Data management; big data issues; storage and back-up, use of public databases, metadata, digital image production, promoting reproduction by others).
Faculty Participation: Again team teaching for the three areas: 1) Matthew O’Connell and David Dominguez Sola; 2) Amaia Lujambio and Emma Benn; 3) Eric Sobie, Marc Birtwistle, Evren U. Azeloglu.
Duration: The course runs for a total of 8 hours over 4 weeks in the Fall semester. Attendance is mandatory. Refresher after 4 years.
Frequency: Twice per week (Monday and Wednesday)
Last Update: March 2019
The Icahn School of Medicine at Mount Sinai (the “School”) has established the Dean's COREs and Shared Research Resources Program to provide substantial support for innovative, state-of-the-art technologies, and technical assistance essential to our research programs in Translational and Basic Sciences. The compact size of Mount Sinai makes it possible for all investigators to have essentially unlimited and easy access to these resources.
Support for the COREs by The School, described below, totals approximately $1.5 million per year, of which user fee charges to investigators recovers 70% of direct costs. The School subsidizes the remaining 30 percent. This makes it possible to charge investigators reduced fees, which promotes increased use of the facilities, and to prevent duplication of these technologies in individual departments or laboratories. An important ancillary result is that the COREs enhance the potential for collaboration in multidisciplinary research programs, since CORE directors can bring together investigators working in similar research areas. This also facilitates the adoption of new experimental approaches by PI’s, especially in situations where crossover requiring basic science data is needed to underpin and to test clinical hypotheses. Thus, Icahn School of Medicine views its commitment to the COREs as a significant part of its cost sharing.
A website was developed that is linked to individual COREs. On the individual websites, there is information about the services, fees, protocols for preparations and where appropriate, the opportunity to sign up for usage on a web-based calendar.
Structure and Governance of the Dean's COREs and Shared Research Resources Program
The Program reports to the Dean for Research Operations and Infrastructure, Reginald W. Miller, DVM, DACLAM. The program is administered through the Dean's Office by the Core Administration , Veronica Moses (Financial Director), Shekhar Patil, PhD (Administrative Director). . Operational oversight is through a dual, peer review mechanism. Each CORE has a dedicated Scientific Advisory Committee chosen from its user group. COREs are reviewed on a two to five-year cycle, External Reviews. The external review criteria include publication credits, technology, and personnel expertise. These reviews assist the institution in determining the need for continued support, emerging trends in technology, and obsolescence of technology. Fiscal oversight and administrative support for the COREs is provided through the office of the Senior Vice President for Finance, Mr. Stephen Harvey via Mrs. Veronica Moses. Dr. Miller works closely with this group to monitor the finances of the COREs. Each core is directed by a Scientific Director and Staff who provides research services, educates investigators on the use of the technology, and helps them develop studies using new technologies in support of their research projects. A senior member of the veterinary staff also serves as ex-officio on all the advisory committees of animal-based COREs. Advisory committees meet quarterly to evaluate and recommend improvements and updates to Core technology and services.
Instrument capital costs are not recovered through user fees but rather through instrumentation grants, Dean’s funds or Departmental contributions with matching funds from the Dean.
The Flow Cytometry SRF provides instrumentation and expertise for automated cytofluorimetric analysis and the sterile sorting of specific cell types. The equipment of this facility is state-of-the art; it consists of multiple high-speed cell sorters and analytical flow cytometers with the capacity to analyze up to 18 simultaneous fluorescent colors in real time with data rates exceeding 10,000 per second.
Major instruments provide for cell sorting by trained operators, including four BD FACSAria II high-speed cell sorter and a 6-way BD Influx high-speed cell sorter. For cell analysis, the equipment is available continuously to trained operators, including BD FACSCantoII and BD LSRIIinstruments. All of the BD FACSAria II cell sorters reside within Class II biosafety cabinets, allowing for isolation of unfixed human samples, including samples from HIV patients. The need for contained sorting of live human cells is increasing rapidly along with the emphasis on translational research. Sorting of human samples on cell sorters in conventional rooms is widely deemed unsafe because the sorting process generates an aerosol, and carries the risk of the transmission of pathogens to users and operators. The BSL2 cell sorting facility was completely funded by the institution ($1.8M) and is absolutely critical to the efforts of the institution in translational research, in particular in the areas of stem cell biology, immunology, cancer, and infectious diseases.
Biorepository and Pathology
The facility provides a consultative service to determine investigator requirements for human tissue and fluid biospecimens in translational research, and then arranges procurement as feasible. After meeting with the investigators about their respective research needs, Core personnel determine the best mechanism to obtain samples and implement the collection. This is made possible by the physical location of this Dean's Core in the Icahn School of Medicine Department of Pathology, and the ability to collect both deidentified and consented 'waste' biospecimens not required for clinical diagnosis. If the requested specimens are not feasible through the Mount Sinai health care system alternative sources will be explored.
The facility also offers basic histology services with the capability to process, embed and section fixed and frozen tissues for the Mount Sinai research community. We also prepare unstained slides suitable for a variety of applications such as histochemistry, immunohistochemistry, immunofluorescence, in situ hybridization as well as hematoxylin and eosin (H&E) stained sections and basic histochemical stains for routine light microscopic evaluation. The Core has the requisite equipment for a fully operational translational research laboratory including tissue processor, embedding station, microtome, cryostat, automated immunostainer, complete digital scanning system, image analysis software, and a semi-automated tissue micro-arrayer. The Core is also able to provide DNA / RNA extractions from all tissue types as well as blood samples and has the capabilities to routinely isolate circulating tumor cells, cell-free DNA as well as serum / plasma.
PDFs provided on the Cores website instruct and guide users on "Human Tissue and Fluids Handling Risks and Safety Precautions", "Human Specimen Single User Agreement" and "Commercial Use Policy and Disclosure". The Core serves to educate investigators about the appropriate human subjects considerations when requests for human materials are made, thus assuring that all human biospecimen applications are in full compliance with HIPAA and IRB regulations.
The use of gamma irradiation to the whole body in animals (rodents) and/or cells has been a major research model for many years. The Mount Sinai Medical center hosts one Precision X-ray X-RAD320 machine in support of this research. A dedicated technician has been hired and trained to oversee the equipment, but more importantly to assist researchers with their studies. Rather than training new post-docs and students on the use of the machine(s), the technician performs all exposures. The result has been greater consistency and reduced down time for the machines. This machine is housed within the central vivarium due to the proximity of the animals used in the various studies. A separate entrance has been designed such that non-animal users are not required to enter the main animal barriers. Please visit the website or email Kevin Kelley for more information.
The Microscopy Core and Advanced Bioimaging Center provides access to high-end instrumentation for light and electron microscopy and to computer workstations for image processing, image deconvolution, and image analysis. Core scientists provide expertise, hands on training, full service electron microscopy preparation, and free consultation services in support of studies using fixed and living preparations. Core scientists can also be engaged collaboratively to carry out broader experimental initiatives and novel lines of investigation. Instrumentation includes three conventional laser scanning confocal microscopes (Zeiss LSM780 and two Leica TCS SP5 microscopes) and two with super-resolution capabilities (Leica TCS SP8 3X STED and Zeiss LSM 880 with Airyscan); one Yokogawa CSU-XI spinning disk microscope; two electron microscopes (Hitachi 7000 and Hitachi 7700); an Olympus FV1000 MPE multiphoton microscope equipped for in vivo imaging; a LaVision UltraMicroscope II light sheet microscope; five widefield systems differentially equipped for tiled acquisition, structured illumination, time lapse imaging and/or polarized light imaging (two Zeiss AxioImagers.Z2 with ApoTome2; one Leica DM6000; one Olympus IX70, one Zeiss Axioplan2); and one macroscope equipped for brightfield and fluorescence acquisition (Olympus MVX10). Automated long term, multi-field time lapse recording of living cells can be accomplished on several systems equipped with motorized stages, autofocus, filters, shutters, fluorescence and brightfield optics, and stage-top incubators. An anesthesia delivery system is available for intravital imaging. Image Analysis workstations are equipped with Imaris, Arivis Vision4D and MetaMorph and AutoQuant X3, Huygens Professional and Leica Lightening are all available for image deconvolution. All microscopes and workstations interface with Acquifer HIVE which provides centralized data storage and high-end image processing. All instruments and workstations are accessible to trained users 24/7.
Mouse Genetics and Gene Targeting
The institutional Mouse Genetics and Gene Targeting (MGGT) CORE has been established to provide the Mount Sinai research community with access to state-of-the-art facilities for the production of transgenic and gene targeted mice, as well as related rodent embryology techniques, on a fee-for-service basis. The MGGT CORE can produce transgenic mice by injection of DNA fragments of varying length (including bacterial artificial chromosomes) into pronuclear stage single-cell mouse embryos. The facility uses CRISPR technology to do genome editing in vivo to create various gene-targeted mouse models, such as knock-outs or knock-ins. The facility can also assist laboratories with the production of targeted mutant lines of mice through the use of mouse embryonic stem (ES) cells. Targeted clones are used to create novel lines of mice by injection of ES cells into blastocysts to create chimeric mice which are mated with wild-type mice to establish germline transmission of the targeted gene. In addition to the creation of novel transgenic and targeted mutation lines, the MGGT CORE can assist laboratories with the maintenance of existing lines through the cryopreservation of sperm. Cryopreservation of mouse sperm allows investigators to successfully bank lines that are not in active use. Shipment of cryopreserved sperm can also serve as an alternative method for transferring lines to collaborators at other institutions. Through collaboration with the Center for Comparative Medicine and Surgery (CCMS), the MGGT CORE also provides investigators with the means to import novel lines from other institutions by recovering mice from cryopreserved embryos or sperm, live embryos, or through the use of IVF rederivation of individual males that are shipped to Mount Sinai. This service is an excellent alternative to the importation of rodents via live animal quarantine. The staff of the MGGT CORE is also available to assist investigators with other rodent surgical techniques, such as vasectomies, ovariectomies, ovary transplants, etc.
Quantitative PCR CoRE The qPCR CoRE provides access to fully automated instruments for real-time PCR, droplet digital PCR, Nanostring nCounter analysis, single cell gene expression analysis, automated nucleic acid isolation, and robotic pipetting. Our facility offers services involving Nanostring nCounter assays, single cell gene expression analysis, qPCR assay development, allelic discrimination assays, nucleic acid extractions, setting-up qPCR reactions, and qualitative and quantitative RNA/DNA analysis. The facility also offers custom services and bioinformatics support for real-time PCR and digital nCounter mRNA quantification in small- to large-scale experiments.
Neuropathology Brain Bank & Research CoRE
This new research laboratory was launched in response to the Mount Sinai Strategic Plan in 2018. The goal of the core is to provide infrastructure to extend Mount Sinai’s leadership in neuropathology-focused research. The new core, with consists of approximately 1000 ft2 is equipped with a broad range of instrumentation for automated processing and staining of human brain tissues. The NBBRC is also equipped to process animal model and cell culture tissues related to neuropathology research. The core is staffed with a team of expert neuropathologists and histotechnologists paired with state-of-the-art techniques and methodology. The facility performs routine histology and special stains (e.g., immunofluorescence and immunohistochemistry). The core also provides high-throughput DNA/RNA extraction from fluid and tissue specimens. In addition, the division of neuropathology maintains a collection of well-characterized and carefully curated brain tissues for research. Tissues include a range of normal human and age-related diseases, including neurodegenerative diseases. Neuropathology core services include:
- Tissue trimming, cassetting, processing, and embedding
- Cutting and routine staining of paraffin-embedded and frozen OCT sections
- Large format tissue processing
- Immunohistochemistry for both routine and novel markers on our BenchMark XT system (Ventana)
- Immunofluorescent staining
- In situ hybridization
- Routine stains (e.g., H&E, LFB, silver, trichrome, etc.)
- DNA/RNA isolation on our King Fisher Flex Purification System (Fisher Scientific)
Hess Freezer Farm
The Freezer Farm Dean’s CoRE at the Icahn School of Medicine is a secure, safe, state of the facility located in the Hess Building, which is fully equipped to meet scientific and research requirements. In the event of limited laboratory space, researchers have the option to relocate their valuable short and long- term sample inventory to the Hess Freezer Farm. In order to ensure the safety and preservation of the materials stored, the Hess Freezer Farm offers a variety of support systems and services including but not limited to emergency electrical back-up system, 24/7 temperature monitoring with on-and off-site call out system and emergency transport, available temperature recordings on all systems, freezer maintenance as necessary, access to dry ice and shipping materials, week-day access with support staff ready to assist, bar-code inventory services and specimen aliquoting services are also available as needed.
Last Update: March 2019
The Icahn School of Medicine at Mount Sinai (ISMMS) ranks 12th on NIH Reporter among the nation's 146 medical schools in receipt of research support from NIH and administers more than 600 NIH grants and contracts awarded to over 350 Principal Investigators. The National Institutes of Health (NIH) funding to ISMMS totaled $348.5 million in Fiscal Year 2018, an increase of 9.4 percent from the previous year and 84.7 percent since 2008.
Last Update: March 2019