The main objective of Core B is to offer specialized scientific, technical, and clinical expertise and services in advanced mouse and human skin research.
Core B Leadership
Director: Michael Rendl, MD, Professor of Cell, Developmental, and Regenerative Biology and Dermatology and Associate Director of the Black Family Stem Cell Institute
Associate Directors: Samuele Marro, PhD, Assistant Professor and co-director of the Stem Cell Engineering Core and Brian S. Kim, MD, MTR, Professor of Dermatology and Director of the Mark Lebwohl Center for Neuroinflammation and Sensation
Core B Services
Core B provides services including:
- Provide clinical and histopathological expertise for easy retrieval and analysis of normal and diseased human skin.
- Train and provide access to Multiplexed Fluorescence In situ Hybridization (merFISH) for spatial analyses of multiple proteins in skin disease studies.
- Provide scientific expertise with genetic mouse models for analyzing mechanisms underlying skin development and homeostasis.
- Consultation for isolation and analysis of skin cells by fluorescence activating cell sorting (FACS) technology.
- Provide expertise with live imaging technology for ex vivo and in vivo skin cell behavior studies.
- Provide support with multiplexed gene targeting with lentiviral intrauterine injection technology.
- Provide expertise with neuronal calcium imaging.
- Scientific expertise and services for human skin disease modeling with generation and characterization of patient-derived Induced pluripotent stem cells (iPSC).
- CRISPR-based gene editing of human pluripotent stem cells (PSC) including the generation of fluorescent reporter alleles, loss-of-function alleles (knock-out), single-nucleotide changes (knock-in) and CRISPR-inhibition (CRISPRi) or CRISPR-activation (CRISPRa).
- Differentiation of PSC to keratinocyte-like cells with transcriptional profile that closely resemble primary keratinocytes.
- Differentiation of PSC to neuronal cells including glutamatergic and GABAergic neurons.
- Differentiation of PSC to other cell types including immune-cells such as monocytes/macrophages and microglia.
To learn more about services involving physiological studies of mouse skin, please contact Dr. Rendl, michael.rendl@mssm.edu.
To learn more about iPSC/gene-editing services, please contact Dr. Marro, samuele.marro@mssm.edu or visit the Stem Cell Engineering Core website.