We at the Icahn School of Medicine at Mount Sinai (ISMMS) provide support for innovative cancer research through the following shared resources:
The Department of Oncological Sciences now offers greater ease of generating cell suspensions from tissues and magnetic cell separations with the gentleMACS™ Octo Dissociator with Heaters, and the autoMACS® Pro Separator (Miltenyi). All reagents, columns and tubes are stocked by the core and available for use at low cost. Numerous dissociation and separation kits are available for each study's specific needs, which may be purchased through Miltenyi by individual users. For more information, please contact Dr. Mihaela Skobe at firstname.lastname@example.org.
Cancer Cell Organoid Core
The Cancer Cell Organoid Core (CCOC) is an Oncological Sciences resource for the establishment of human tumor lines from ISMMS clinical specimens. New techniques in sample processing and cell culture methods have made it possible to reproducibly establish such lines as novel in vitro models of studying disease and modeling therapeutic strategies that may one day be applied clinically.
Presently, breast tumors of different subtypes and stages are being explanted into culture on an as available basis. Researchers can submit a short research proposal to the CCOC to obtain such cultures for their investigations. Culture reagents are the responsibility of the individual researchers, but protocols will be provided to assist researchers in maintenance of these cells.
Contact Pamela Cheung at email@example.com for additional information.
We maintain a state-of-the-art cryogenics facility for long-term storage of cell lines by ISMMS investigators. We can accommodate deposit and delivery of cells as well as viable freezing of cells on a regularly scheduled basis. You can rent a safety deposit box, which contains space for 81 individual vials for $60 per year, and you can negotiate discounts for larger needs. We can provide back-up to investigators who have their own freezers or serve as the primary means of viable cell storage. A cost estimate can be obtained by filling out a Cryogenic Facility Rental Request Form.
Contact Ed Hicks at firstname.lastname@example.org for additional information.
We provide tissue, embedding, sectioning and histochemistry services in support of ISMMS investigators involved in grant or institutionally funded cancer-related projects using a charge-back system for technologist time and effort. You can submit a request for limited support of non-funded pilot studies that are are aimed at developing preliminary data for cancer-relevant grant proposals. We can arrange for pathology consultation on an ad hoc basis. For a cost estimate, fill out a Histo-Immunology Lab Worksheet.
Contact Rui Qiao at email@example.com for additional information.
We offer fully automated immunohistochemistry (IHC), immunofluorescence staining (IF), and in situ hybridization (ISH) on cells and tissues. Our Immunostaining facility, managed by Mihaela Skobe, PhD, is equipped with a LeicaBond RX instrument (Leica Biosystems) that can stain up to 30 slides per run while testing multiple protocols simultaneously. The LeicaBond instrument offers the speed and flexibility required to optimize new antibodies or to process numerous slides/protocols in a timely manner with high-quality results. We can stain both frozen and FFPE tissues. We offer both validated protocols as well as direct assistance with protocol development.
For consultation and scheduling, contact Mihaela Skobe at firstname.lastname@example.org. You can reserve the LeicaBond RX online through eRAP.
Laser Capture Microscopy
We offer laser capture microscopy for micro-dissection of tissue sections. You can use this state-of-the-art equipment to identify and isolate fluorescent-labeled cells in tissue sections. We offer a free training session to allow investigators to use the equipment for a nominal charge ($25/hour). Our department technologists are also available to perform micro-dissection on a charge-back basis for time and effort. You can arrange for pathology consultation on an ad hoc basis.
Contact Rui Qiao at email@example.com for a cost estimate.
The core has a Nextseq 500 from Illumina and requisite instruments for deep-sequencing sample prep, including the Agilent 2100 bioanalyzer (RNA/DNA QC), the Covaris M220 sonicator (RNA/DNA fragmentation) and the Qubit fluorometer 2.0 (nucleic acid quantification). The core exists to facilitate research and enable method development by allowing small experimental runs (as little as 10 million reads/sample) and ensuring quick turn-around times (usually a week from sample -> fastq files). A variety of reads lengths are possible (75 bp, the most popular, 150 bp and 300 bp) in paired-end or single-end mode with single or dual indices allowing for massive multiplexing (practically, anything more than 50 samples per run might be difficult to manage). We are also actively developing new techniques and implement new technologies as they become available in the market.
A 400 MHz NMR instrument (Bruker Avance III HD) is available for structural analysis and validation by 1D (NOE, APT, DEPT) and 2D (COSY, NOESY, HSQC, HMBC) experiments. The 400 MHz NMR has a room temperature BBFO probe for spectra of high band (1H, 19F) and low band (13C, 15N, 31P) nuclei. For more information, please contact Dr. Arvin Dar at firstname.lastname@example.org.
The facility operates a suite of Agilent (Seahorse) XF Analyzers that use label-free technology to detect discrete changes in cellular bioenergetics in real-time, providing a window into the critical functions driving cell signaling, proliferation, activation, toxicity, and biosynthesis. Real-time measurements of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) are made by isolating an extremely small volume (about 2 μL) of medium above a monolayer of cells within a micro plate. The facility currently hosts XFe96, XFe24, and XFp machines, and routinely uses XF Glycolytic Rate, Cell Mito Stress, and Cell Energy Phenotype Test Kits. We provide a combination of access, training, collaborations, and experimental consumables. Contact the Agilent website for further information related to the analyzers, consumables, and applications (https://www.agilent.com/en-us/products/cell-analysis-(seahorse).
Complete the request form to initiate project
Contact Jerry Edward Chipuk, Ph.D. at Jerry.email@example.com