Services and Equipment

The Biorepository and Pathology CoRE is a multi-dimensional service group providing consultation and technical support for the translational and basic science groups across the Mount Sinai Health System. 

If you have any questions regarding these services, please contact  Rachel Brody, MD, PhD at rachel.brody@mountsinai.org .

Please note: it is important that you acknowledge the Biorepository and Pathology CoRE in all publications and grants that include data derived from our facility.

We offer basic histology services, including processing, embedding and sectioning of frozen and fixed (human and animal) tissue samples. We provide hematoxylin and eosin stained slides, as well as multiple special stains (e.g. Acid Fast Bacilli (AFB), Periodic Acid Shift (PAS), Giemsa, Gram, Masson’s trichrome etc...). We can generate unstained sections for a variety of applications, such as immunohistochemistry, in situ hybridization, laser capture microdissection, etc.). We also offer the generation of tissue microarrays (TMA) from different tissue blocks.

Equipment

  • Galileo CK4600 Tissue Microarray System: Advanced EDM Automation Tissue Array High-throughput construction of customized tissue array. Involves multiple tissue samples extracted from donor paraffin-embedded tissue blocks into a single paraffin block.
  • Sakura E300 Tissue Processor: Automatically process tissue samples for embedding by dehydration after fixation in 10 percent Neutral Buffer Formalin
  • Sakura Tissue-Tek Embedding Center: Used to embed the processed tissue samples in paraffin
  • Leica Cryostat: To section frozen tissue blocks for further use in a variety of applications

If you have any questions regarding these services, please email Fran Avila or Alan Soto, or call them at 212-241-7582.

The Pathology CoRE provides a variety of immunostaining services, including manual or automated (Ventana system, Roche) immunohistochemistry and immunofluorescence using both commercial and in-house antibodies. We also provide antibody characterization/titration/interpretation and imaging services, as well as rapid immunohistochemistry staining for laser capture microdissection. We perform both manual and automated (Ventana system, Roche) in situ (RNA / DNA) hybridization.

Equipment

  • Discovery Ultra: Automated Immunohistochemistry (IHC)/In situ hybridization (ISH) research slide staining system. For research application including IHC, multiplex Immunofluorescence, DNA / RNA ISH

If you have any questions regarding these services, please email Tin Htwe Thin or call 212-241-6050.

The Pathology CoRE provides laser capture microdissection services from frozen and or paraffin-embedded tissue samples as well as cells. We also offer training to all investigators to use the system. We can provide sectioning of samples onto special membrane-coated slides and rapid immunostaining as required.

Equipment

  • Leica Microsystems LMD6500 Laser Microdissection: For specimen collection by gravity contact, contamination-free to obtain ultra-pure sample of single cells, both from tissue samples and cells.

If you have any questions regarding these services, please email Tin Htwe Thin or call 212-241-6050.

Whole Slide Scanning and Image Analysis

The Pathology CoRE offers whole slide (digital) scanning and image analyses services using the Olympus microscope scanner and Visiopharm Integrator software, from both whole sections and tissue microarrays.

Equipment

  • Pannoramic 250 FLASH II (Digital Scanner): We have a Perkin Elmer Digital Slide Scanner with capacity for Bright Field or Immunofluorescent image capture at 20x and/or 40x magnification. Our system includes the Pannormaic Viewer for Image Analysis (Cell Quant and Pattern Quant) and Immunofluorescent filters (DAPI, FITC, CYC, CY5).
  • Olympus scanner with Visiopharn Integrator Software System: It provides a digital scanned image of both whole and TMA sections as well as automation image analysis of whole slide / TMA images. Visio Pharm Software: Quantitative assessment of H&E and immunostained regions to create quantitative biomarker features such as area and intensity.
  • Olympus confocal microscope, equipped with a variety of filters to evaluate different fluorescent probes.

If you have any questions regarding these services, please email Tin Htwe Thin, PhD or Roni Sussman, or call them at 212-241-7582.

The Pathology CoRE provides DNA and RNA/ miRNA extraction including quality/quantitation services from a variety of specimen sources including blood, buffy coat, frozen/fixed tissues, swabs, spit cups and brushes.

Equipment

  • NanoDrop 2000 spectrophotometer Thermo Scientific:  To assess DNA/RNA quality-quantity after extraction.
  • Maxwell RSC48 and Maxwell RSC 16 automated nucleic acid purification system

For more information, please email Yayoi Kinoshita, DMD or call her at 212-241-4414.

To request specimens from the Biorepository please email Rachel Brody, MD PhD or call her at 212-241-0678; ext. 40678. You will be asked to provide an abstract or specific aims that clearly describe the project for which you will use human tissues or fluid, including a detailed description of collection requirements; grant support information for your research program; assurance of institutional IRB approval, GCO fund number. You will also be asked to complete forms acknowledging your responsibility in understanding of and adherence to appropriate safety standards for the protection of yourself and others while handling human tissues; acknowledging that you will not further distribute the materials disbursed to you by the Biorepository; and agreeing to provide specific acknowledgement to the Mount Sinai Biorepository in any publications or grants related to the use of the tissues you receive.