Services and Equipment

The Biorepository and Pathology CoRE is a multi-dimensional service group providing consultation and technical support for the translational and basic science groups across the Mount Sinai Health System. 

If you have any questions regarding these services, please contact  Rachel Brody, MD, PhD at rachel.brody@mountsinai.org .

Please note: it is important that you acknowledge the Biorepository and Pathology CoRE in all publications and grants that include data derived from our facility.

We offer basic histology services, including processing, embedding and sectioning of frozen and fixed (human and animal) tissue samples. We provide hematoxylin and eosin stained slides, as well as multiple special stains (e.g. Acid Fast Bacilli (AFB), Periodic Acid Shift (PAS), Giemsa, Gram, Masson’s trichrome etc...). We can generate unstained sections for a variety of applications, such as immunohistochemistry, in situ hybridization, laser capture microdissection, etc.). We also offer the generation of tissue microarrays (TMA) from different tissue blocks.

Equipment

  • Galileo CK4600 Tissue Microarray System: Advanced EDM Automation Tissue Array High-throughput construction of customized tissue array. Involves multiple tissue samples extracted from donor paraffin-embedded tissue blocks into a single paraffin block.
  • Sakura E300 Tissue Processor: Automatically process tissue samples for embedding by dehydration after fixation in 10 percent Neutral Buffer Formalin
  • Sakura Tissue-Tek Embedding Center: Used to embed the processed tissue samples in paraffin
  • Leica Cryostat: To section frozen tissue blocks for further use in a variety of applications

If you have any questions regarding these services, please email Fran Avila or Alan Soto, or call them at 212-241-7582.

The Pathology CoRE provides a variety of immunostaining services, including manual or automated (Ventana system, Roche) immunohistochemistry and immunofluorescence using both commercial and in-house antibodies. We also provide antibody characterization/titration/interpretation and imaging services, as well as rapid immunohistochemistry staining for laser capture microdissection. We perform both manual and automated (Ventana system, Roche) in situ (RNA / DNA) hybridization.

Equipment

  • Discovery Ultra: Automated Immunohistochemistry (IHC)/In situ hybridization (ISH) research slide staining system. For research application including IHC, multiplex Immunofluorescence, DNA / RNA ISH

If you have any questions regarding these services, please email Tin Htwe Thin or call 212-241-6050.

The Pathology CoRE provides laser capture microdissection services from frozen and or paraffin-embedded tissue samples as well as cells. We also offer training to all investigators to use the system. We can provide sectioning of samples onto special membrane-coated slides and rapid immunostaining as required.

Equipment

  • Leica Microsystems LMD6500 Laser Microdissection: For specimen collection by gravity contact, contamination-free to obtain ultra-pure sample of single cells, both from tissue samples and cells.

If you have any questions regarding these services, please email Tin Htwe Thin or call 212-241-6050.

Imaging
The Biorepository and Pathology CoRE offers quick imaging of IHC stained slides using Nikon EclipseCi-L upright clinical microscope.
Equipment
*Nikon Eclipse Ci-L with capacity for bright field image captured at 4X, 10X, 20X, 40X and or 60X magnification.
If you have any questions regarding imaging service please email or call to Tin at (212) 241-6050.

Scanning
The core offers whole slide (digital) scanning services using NanoZoomer S210 Digital slide scanner from both whole tissue sections, tissue microarrays and cytology specimens.
Equipment
*NanoZoomer S210 Digital slide scanner (C13239-01):We have a Hamamatsu Digital Slide Scanner with capacity for Bright Field image capture at 20X and/or 40X magnification. The system includes NDP.view2 Viewing software.
If you have any questions regarding scanning service, please email or call to Alan at (212) 659-9376 or Roni at 212-241-7510.

Image Analysis
The Pathology Core offers quantitative tissue image analysis platform using HALO®, the Indica Lab. The platform offers 5 different analysis: Cytonuclear IHC, Multiplex IHC (up to 5 chromogenic markers expression in nucleus, cytoplasm or membrane), Tissue Classifier for object based analysis, Spatial analysis for Immuno-Oncology and TMA.
If you have any questions regarding image analysis service, please email or call to Tin at (212) 241-6050.

The Pathology CoRE provides DNA and RNA/ miRNA extraction including quality/quantitation services from a variety of specimen sources including blood, buffy coat, frozen/fixed tissues, swabs, spit cups and brushes.

Equipment

  • NanoDrop 2000 spectrophotometer Thermo Scientific:  To assess DNA/RNA quality-quantity after extraction.
  • Maxwell RSC48 and Maxwell RSC 16 automated nucleic acid purification system

For more information, please email Yayoi Kinoshita, DMD or call her at 212-241-4414.

To request specimens from the Biorepository please email Rachel Brody, MD PhD or call her at 212-241-0678; ext. 40678. You will be asked to provide an abstract or specific aims that clearly describe the project for which you will use human tissues or fluid, including a detailed description of collection requirements; grant support information for your research program; assurance of institutional IRB approval, GCO fund number. You will also be asked to complete forms acknowledging your responsibility in understanding of and adherence to appropriate safety standards for the protection of yourself and others while handling human tissues; acknowledging that you will not further distribute the materials disbursed to you by the Biorepository; and agreeing to provide specific acknowledgement to the Mount Sinai Biorepository in any publications or grants related to the use of the tissues you receive.