Flow Cytometry Equipment and Rates | Icahn School of Medicine

RESOURCES

Dean’s CoREs

To provide the best range of cytometric capabilities and applications, the Flow Cytometry CoRE is comprised of multiple machines from established manufacturers. All our analyzers and cell sorters use a detector configuration that allows for template portability, as well as preventing figures and data from being mislabeled in publication. We have provided a list of the most common fluorochromes in each detector so investigators can choose the appropriate instrument for their samples.

Rates and Cancellation Charges

Our rates for each instrument are indicated at the point of reservation within iLab. All reservations are subject to a 24-hour advance cancellation period. Cancellation within 24 hours of the scheduled start of a session will result in a charge equal to the entire scheduled session time. This cancellation charge will be pro-rated based upon assumption of the cancelled time by another user.

Data Workstations and Software

The Flow Cytometry CoRE maintains several workstations to analyze data generated within the CoRE, all of which are available to use at no charge. These PC workstations have up-to-date versions of FlowJo, IDEAS, DiVa, Spectroflo, and access to cloud services such as EasyPanel.

The CoRE also provides a site license for FlowJO, a powerful flow cytometry analysis software. Researchers can obtain a license through the iLab system's Software Licenses section, found at the bottom of the Request Services tab.

Equipment Overview

Below are the various types of analyzers we employ.

Canto II - Manufactured by BD, this instrument features three lasers and eight colors, as well as semi-automated fluidic operation.
LSRFortessa X-20 - Manufactured by BD, this instrument features five lasers and 18 colors for maximum complexity of experimental design.
Attune NxT - Manufactured by ThermoFisher, this instrument features four lasers and 14 colors for maximum complexity of experimental design.
Aurora - Manufactured by Cytek Biosciences, this instrument features five lasers and spectral detection technology to facilitate more than 60 discrete multiplexed probes per cell.
ImageStream - Manufactured by Luminex/Amnis, this instrument features six lasers and 12 spectral channels plus bright and darkfield for high speed image capture of individual cells in suspension. The number of discrete parameters per cell can be greater than 50 based on image features.
BD FACSymphony A5SE - 48 fluorescent channels that allow to : Gain full visible spectrum coverage to collect all light emitted without the need for filter changes, increasing dye flexibility and number of simultaneous colors. DIVA software Choose between spectral or compensation-based workflows, achieved through careful optical design and added software functionality link

Our CoRE works with eight different cell sorters, as detailed below.

CS - Manufactured by CytekBiosciences, this instrument features five lasers and full spectrum detection technology to provide up to 60 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
SRT - Manufactured by Beckman Coulter Life Sciences, this instrument features five lasers and 15 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
IML4 - Manufactured by BD, this instrument features four lasers and 12 colors inside a biosafety cabinet for sorting human or potentially infectious material.
Tyto - Manufactured by Miltenyi, these identical instruments feature cartridge based microfluidic sorting for true sterility. The optics are configured with three lasers and eight fluorescence detectors, as well as three backscatter detectors. These instruments are preferentially scheduled for infectious material sorting.
FACSDiscover S8 . BD FACSDiscover™ S8 Cell Sorter with BD CellView™ Image Technology and BD SpectralFX™ Technology, the first spectral flow cytometer sorter with sort-capable image analysis, expands the power of cell analysis and sorting to new dimensions by combining spectral flow cytometry with real-time spatial and morphological insights. This sorter is contained within a biosafety cabinet and allows human and virus treated material.” Should be added to the list.
FACSymphony S6 . This is 5 laser sorter with 23 fluorescence detectors. It has a 6-way sorting option as well as capability to collect cells in 1.5, 5, 15 and 50 ml tubes. This sorter is contained within a biosafety cabinet and allows human and virus treated material.