Flow Cytometry Equipment | Icahn School of Medicine

RESOURCES

Dean’s CoREs

To provide the best range of cytometric capabilities and applications, the Flow Cytometry CoRE is comprised of multiple machines from established manufacturers. All our analyzers and cell sorters use a detector configuration that allows for template portability, as well as preventing figures and data from being mislabeled in publication. We have provided a list of the most common fluorochromes in each detector so investigators can choose the appropriate instrument for their samples.

Rates and Cancellation Charges

Our rates for each instrument are indicated at the point of reservation within iLab. All reservations are subject to a 24-hour advance cancellation period. Cancellation within 24 hours of the scheduled start of a session will result in a charge equal to the entire scheduled session time. This cancellation charge will be pro-rated based upon assumption of the cancelled time by another user.

Data Workstations and Software

The Flow Cytometry CoRE maintains several workstations to analyze data generated within the CoRE, all of which are available to use at no charge. These PC workstations have up-to-date versions of FCS Express, IDEAS, DiVa, Spectroflo, and access to cloud services such as Fluorofinder and Cytobank, as well as Microsoft Office. Other data analysis packages may become available as beta test versions predicated on the Icahn School’s reputation for high volume flow cytometry and broad subject matter. Currently, five workstations are placed in IMI13-05, and two in CSM S6-308 for use at any time.

The CoRE also provides a site license for FCS Express Version 7, a powerful flow cytometry analysis software. Researchers can obtain a license through the iLab system's Software Licenses section, found at the bottom of the Request Services tab. This grants access to additional resources for getting started with FCS Express.

Equipment Overview

Below are the various types of analyzers we employ.

Canto II - Manufactured by BD, this instrument features three lasers and eight colors, as well as semi-automated fluidic operation.
LSRFortessa X-20 - Manufactured by BD, this instrument features five lasers and 18 colors for maximum complexity of experimental design. This instrument has a high-throughput screening (HTS) option available to read samples from 96 and 384 well microtitre plates.
Attune NxT - Manufactured by ThermoFisher, this instrument features four lasers and 14 colors for maximum complexity of experimental design.
Aurora - Manufactured by Cytek Biosciences, this instrument features five lasers and spectral detection technology to facilitate more than 60 discrete multiplexed probes per cell.
ImageStream - Manufactured by Luminex/Amnis, this instrument features six lasers and 12 spectral channels plus bright and darkfield for high speed image capture of individual cells in suspension. The number of discrete parameters per cell can be greater than 50 based on image features.

Our CoRE works with eight different cell sorters, as detailed below.

CS - Manufactured by CytekBiosciences, this instrument features five lasers and full spectrum detection technology to provide up to 60 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
SRT - Manufactured by Beckman Coulter Life Sciences, this instrument features five lasers and 15 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
IMI5L - Manufactured by BD, this instrument features four lasers and 12 colors inside a biosafety cabinet for sorting human or potentially infectious material.
CSM5L - Manufactured by BD, this instrument features five lasers and 16 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
Syd - Manufactured by BD, this instrument features four lasers and 16 colors inside a biosafety cabinet for sorting human or potentially infectious material. This instrument is preferentially scheduled for self-operation.
Tyto181 and Tyto183 - Manufactured by Miltenyi, these identical instruments feature cartridge based microfluidic sorting for true sterility. The optics are configured with three lasers and eight fluorescence detectors, as well as three backscatter detectors. These instruments are preferentially scheduled for infectious material sorting.
Wolf - Manufactured by Nanocellect, this instrument features three colors and microfluidic containment for inherently biosafe sorting. Wolf is currently installed within the Department of Microbiology BSL3 laboratory and requires additional training for facility access.