Step 1: Generate an iLab Account

• Visit the Mount Sinai iLab sign-in page.
• Note: You must have a linked fund to proceed with reservations.

Step 2: Email the Core Contact us at FlowCytometryCoRE@mssm.edu and provide the following details:

• Your lab location (e.g., Icahn, Hess, etc.).
• Your availability over the next few business days.

Step 3: Consultation We will schedule a 30-minute session to cover:

• Protocol discussion and experimental design.
• Instrument selection (e.g., conventional vs. spectral).
• An introduction to the Flow CoRE iLab management system.

Step 4: Training Training requirements are based on your experience level:

• Experienced Users: Maintenance and instrument-specific refresh training (includes startup, shutdown, cleaning and data backup).
• New Users: Complete comprehensive training on the selected instrument.

The Flow Cytometry CoRE is equipped with eight flow cytometers and nine fluorescence activated cell sorting machines in several locations across the Icahn School campus. All equipment is maintained daily by CoRE staff.

• Analyzers (Aurora, Fortessa, CantoII, Attune, A5): $63/hr (Self-Op) | $139/hr (Assisted)
• ImageStream: $100/hr (Self-Op) | $150/hr (Assisted)
• Sorters (S6, CS, S8): $122/hr (Self-Op) | $168/hr (Assisted)
• Tyto: $126/hr (Assisted) + $140/cartridge
• Data Analysis: $158/hr

Booking & Cancellation Policy

• Minimum Booking: 1 hour for Sorters; 30 minutes for Analyzers and Tyto.
• Advance Notice: Sorters and Tyto must be booked at least 5 hours prior.
• Late Cancellations: Cancellations made less than 24 hours before a session incur a 70% charge of the booked time.
• Rebooking Credit: If your canceled slot is rebooked by another user, the original slot will not be charged.

Users are responsible for the immediate collection and backup of their data following acquisition.

The Flow Cytometry CoRE does not provide long-term data storage or archival services. Data on acquisition computers will be deleted on a weekly basis to ensure smooth instrument operation.

Mount Sinai IT provides multiple secure data storage options (e.g., network drives, OneDrive, Teams, Google Drive):

For guidance on selecting the appropriate solution, please contact Sinai IT.

The Flow Cytometry CoRE welcomes opportunities for scientific collaboration on projects requiring substantial involvement in experimental design, assay development, panel design, data analysis and interpretation, or preliminary data generation.

Investigators interested in developing a collaborative project are encouraged to contact the Flow CoRE early in the planning process to discuss the project and the appropriate level of CoRE involvement.

The Flow Cytometry CoRE provides letters of support for grant applications that include use of the CoRE.

To request a letter, please email siu-hong.ho@mssm.edu at least one week before the submission deadline** and include:

* Grant title

* A brief description of the project

* A brief description of how flow cytometry will be used to address the specific aim(s) of the grant

If discussion is needed, please contact us to arrange a consultation.

Please consider including Flow Cytometry CoRE staff as co-authors when they make substantive contributions to a project, based on the Association of Biomolecular Resources Facilities (ABRF) Recommended Guidelines for Authorship on Manuscripts.

Examples include:

1. Conception or design of the project, critical input, or original ideas;
2. Acquisition, analysis, or interpretation of data beyond routine practices;
3. Drafting the article or critically revising it for intellectual content;
4. Writing a portion of the paper beyond the Materials and Methods section;
5. Other substantive intellectual contributions; or
6. Final approval of the article and responsibility for its content.

Routine technical services alone generally do not constitute authorship and should instead be recognized in the acknowledgments, as appropriate.

We encourage investigators to discuss potential authorship with their lab members and Flow CoRE staff early in the project and before manuscript submission.

We are required to acknowledge NIH funding sources in all publications (manuscripts, abstracts, presentations, etc.) that utilize our resources. Please use the following wording in every publication resulting from the use of the Flow Cytometry CoRE instruments:

Acknowledgment Text: "Research reported in this publication was supported by the Flow Cytometry CoRE (RRID:SCR_027701) at the Icahn School of Medicine at Mount Sinai and by the National Cancer Institute of the National Institutes of Health under award number P30 CA196521.  The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."

Please notify the CoRE administration once your manuscript has been accepted for publication by contacting Siu-hong Ho and Shekhar Patil.