The Tisch Cancer Institute (TCI) Flow Cytometry Shared Resource Facility (FCSRF) provides TCI members access to state-of-the-art technology and expertise for the successful development of scientific research projects. Flow cytometry is a powerful technology that enables researchers to study simultaneous multiparametric characteristics of thousands of cells per second to decipher complex cellular and molecular processes in health and disease. The FCSRF offers services for routine applications such as single cell isolation to rapidly isolate live cells, as well as to conduct more innovative and complex biological studies such as RNAseq, metabolomics, and lipidomics assays. The FCSRF is composed of the institutional Flow Cytometry Shared Resource Facility and two TCI satellite sites. The FCSRF is equipped with four analyzers, two mouse cell sorters, three cell sorters contained in biosafety level 2+ areas dedicated to isolate non-fixed human samples and mouse cells infected with class II pathogens, and one mass cytometer (CyTOF). In addition, two satellite sites are equipped with four analyzers. Staffed with seven flow and mass cytometry specialists committed 100 percent to this facility, the SRF provides operational, training, and consultation services covering all aspects of flow cytometry. This includes cell cycle analysis, isolation single cells from cancer patients, and identification of more than 30 different cell surface and intracellular antigens on the same cell using mass cytometry. Our mission is to provide the necessary instrumentation and knowledge regarding flow and mass cytometry and cell sorting so that Tisch Cancer Institute investigators can improve human cancer diagnosis and treatment through basic sciences, translational, and clinical research.
Reservations can be made through the Electronic Research Application Portal (eRAP) website. We charge a minimum fee of one hour for equipment set-up and decontamination time.